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MtDNA control region sequence polymorphisms and phylogenetic analysis of Malay population living in or around Kuala Lumpur in Malaysia

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Abstract

Control region polymorphisms in the mitochondrial DNA of 124 unrelated individuals from the Malay population living in or around Kuala Lumpur in Malaysia were investigated and phylogenetic haplogroup lineages were determined. The intergenic COII/tRNALys 9-bp deletion, 3010 and 5178 mutations, and several coding region polymorphisms were examined to discriminate some phylogenetic haplogroups. Sequence comparison of the control regions led to the identification of 117 mitochondrial haplotypes, in which 103 types were observed in only one individual and the other nine types were shared by more than two individuals. Gene diversity was estimated to be 0.997. Phylogenetic haplogroup determination revealed that the gene pool of the modern Malay population in Malaysia consisted mainly of southeast Asian, east Asian, unidentified and unique, and aboriginal southeast-specific haplogroups. These results suggest a multi-original nature for the modern Malay population. The present database may help not only in personal identification but also in determining geographic origin in forensic casework in Malaysian, Southeast Asian and East Asian populations.

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Acknowledgements

We would like to thank Associate Professor Jeremy Williams, Laboratory of International Dental Information, Tokyo Dental College, for editing this manuscript. This research was partially supported by the Ministry of Education, Science, Sports and Culture; Grant-in-Aid for Scientific Research (B) (14370701); and Grant-in-Aid for Young Scientists Research (B) (29791646).

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Authors and Affiliations

  1. Department of Forensic Odontology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba City, 261-0011, Japan

    S. Maruyama, C. Nohira-Koike & K. Minaguchi

  2. Department of General Dental Practice and Oral & Maxillofacial Imaging, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia

    P. Nambiar

Authors
  1. S. Maruyama
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  2. C. Nohira-Koike
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  3. K. Minaguchi
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  4. P. Nambiar
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Corresponding author

Correspondence to S. Maruyama.

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ESM Table S1

Variable nucleotide positions in 124 Malay samples compared to reference sequences. (XLS 201 kb)

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Maruyama, S., Nohira-Koike, C., Minaguchi, K. et al. MtDNA control region sequence polymorphisms and phylogenetic analysis of Malay population living in or around Kuala Lumpur in Malaysia. Int J Legal Med 124, 165–170 (2010). https://doi.org/10.1007/s00414-009-0355-6

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  • DOI: https://doi.org/10.1007/s00414-009-0355-6

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