Abstract
Using primary explant cultures of mouse bladder, the early response of the urothelium after superficial and full-thickness injuries was investigated. In such an in vitro wound healing model, explant surfaces with a mostly desquamated urothelial superficial layer represented superficial wounds, and the exposed lamina propria at the cut edges of the explants represented full-thickness wounds. The urothelial cell ultrastructure, the expression and subcellular distribution of the tight junctional protein occludin, and differentiation-related proteins CK 20, uroplakins, and actin were followed. Since singular terminally differentiated superficial cells remained on the urothelium after superficial injury (i.e., original superficial cells), we sought to determine their role during the urothelial wound-healing process. Ultrastructural and immunocytochemical studies have revealed that restored tight junctions are the earliest cellular event during the urothelial superficial and full-thickness wound-healing process. Occludin-containing tight junctions are developed before the new superficial cells are terminally differentiated. New insights into the urothelium wound-healing process were provided by demonstrating that the original superficial cells contribute to the urothelium wound healing by developing tight junctions with de novo differentiated superficial cells and by stretching, thus providing a large urothelial surface with asymmetric unit membrane plaques.
Similar content being viewed by others
References
Bement WM, Forscher P, Mooseker MS (1993) A novel cytoskeletal structure involved in purse string wound closure and cell polarity maintenance. J Cell Biol 121:565–578
Fukushima S, Cohen SM., Arai M, Jacobs JB, Friedell GH (1981) Scanning electron microscopic examination of reversible hyperplasia of the rat urinary bladder. Am J Pathol 102:373–380
Hicks RM (1975) The mammalian urinary bladder: an accommodating organ. Biol Rev 50:215–246
Hicks RM, Ketterer B, Warren RC (1974) The ultrastructure and chemistry of the luminal plasma membrane of the mammalian urinary bladder: a structure with low permeability to water and ions. Philos Trans R Soc Lond B 268:23–38
Hu P, Meyers S, Liang F-X, Deng F-M, Kachar B, Zeidel ML, Sun T-T (2002) Role of membrane proteins in permeability barrier function: uroplakin ablation elevates urothelial permeability. Am J Physiol Renal Physiol 283:F1200-F1207
Jezernik K, Romih R, Mannherz HG, Koprivec D (2003) Immunohistochemical detection of apoptosis, proliferation and inducible nitric oxide synthase in rat urothelium damaged by cyclophosphamide treatment. Cell Biol Int 27:863–869
Jost SP (1989) Cell cycle of normal bladder urothelium in developing and adult mice. Virchows Arch B Cell Pathol Incl Mol Pathol 57(1):27–36
Kachar B, Liang F, Lins U, Ding M, Wu XR, Stoffler D, Aebi U, Sun T-T (1999) Three-dimensional analysis of the 16 nm urothelial plaque particle: luminal surface exposure, preferential head-to-head interaction, and hinge formation. J Mol Biol 285:595–608
Kreft ME, Romih R in Sterle M (2002) Antigenic and ultrastructural markers associated with urothelial cytodifferentiation in primary explant outgrowths of mouse bladder. Int Cell Biol 26:63–74
Lavelle J, Myers S, Doty D, Buffington A, Zeidel ML, Apodaca G (2000) Urothelial pathophysiological changes in feline interstitial cystitis: a human interstitial cystitis model. Am J Physiol Renal Physiol 278:F540-F553
Lavelle J, Meyers S, Ramage R, Bastacky S, Doty D, Apodaca G, Zeidel ML (2002) Bladder permeability barrier: recovery from selective injury of surface epithelial cells. Am J Physiol Renal Physiol 283:F242-F253
Lewis SA, Diamond JM (1976) Na+ transport by rabbit urinary bladder, a tight epithelium. J Memb Biol 28:1–40
Lotz MM, Rabinovitz I, Mercurio AM (2000) Intestinal restitution: progression of actin cytoskeleton rearrangements and integrin function in a model of epithelial wound healing. Am J Pathol 156:985–995
Matter K, Balda MS (1999) Occludin and the functions of tight junctions. Int Rev Cytol 186:117–146
McCarthy KM, Skare IB, Stankewich MD, Furuse M, Tsukita S (1996) Occludin is a functional component of the tight junction. J Cell Sci 109:2287–2298
Messier B, Leblond CP (1960) Cell proliferation and migration revealed by radioautography after injection of thymidine-H3 into male rats and mice. Am J Anat 106:247–265
Min G, Zhou G, Schapira M, Sun T-T, Kong X-P (2003) Structural basis of urothelial permeability barrier function as revealed by Cryo-EM studies of the 16 nm uroplakin particle. J Cell Sci 116:4087–4094
Negrete HO, Lavelle JP, Berg J, Lewis SA, and Zeidel ML (1996) Permeability properties of the intact mammalian bladder epithelium. Am J Physiol Renal Fluid Electrolyte Physiol 271:F886-F894
Nusrat A, Delp C, Madara JL (1992) Intestinal epithelial restitution: characterization of cell culture model and mapping of cytoskeletal elements in migrating cells. J Clin Invest 89:1501–1511
Rao JN, Platoshyn O, Li L, Guo X, Golovina VA, Yuan JX-J, Wang J-Y (2002) Activation of K+ channels and increased migration of differentiated intestinal epithelial cells after wounding. Am J Physiol Cell Physiol 282:C885-C898
Romih R, Veranic P, Jezernik K (1999) Actin filaments during terminal differentiation of urothelial cells in the rat urinary bladder. Histochem Cell Biol 112:375–380
Romih R, Koprivec D, Martincic DS, Jezernik K (2001) Restoration of the rat urothelium after cyclophosphamide treatment. Cell Biol Int 25:531–537
Silen W, Ito S (1985) Mechanism for rapid-epithelialization of the gastric mucosal surface. Annu Rev Physiol 47:217–229
Truschel ST, Wang E, Ruiz WG, Leung S-M, Rojas R, Lavelle J, Zeidel M, Stoffer S, Apodaca G (2002) Stretch-regulated exocytosis/endocytosis in bladder umbrella cells. Mol Biol Cell 13(3):830–846
Veranič P, Jezernik K (2000) The response of junctional complexes to induced desquamation of mouse bladder urothelium. Biol Cell 92(2):105–113
Veranič P, Jezernik K (2002) Trajectorial organisation of cytokeratins within the subapical region of umbrella cells. Cell Motil Cytoskeleton 53(4):317–325
Veranič P, Romih R, Jezernik K (2004) What determinates differentiation of urothelial umbrella cells? Eur J Cell Biol 83:27–34
Wu XR, Manabe M, Yu J, Sun T-T (1990) Large scale purification and immunolocalization of bovine uroplakins I, II, and III: molecular markers of urothelial differentiation. J Biol Chem 265:19170–19179
Yu HJ, Chien CT, Lai YJ, Lai MK, Chen CF, Levin RM, Hsu SM (2004) Hypoxia preconditioning attenuates bladder overdistension induced oxidative injury by upregulation of Bcl-2 in the rat. J Physiol 554:815–828
Acknowledgements
The authors thank Prof. Tung-Tien Sun (New York University Medical School) for the generous gift of uroplakin antibodies and Dr. Marko Kreft (LN-MCP Medical Faculty, University of Ljubljana) for the work with the confocal microscope. The study was supported by the Slovenian Ministry of Education, Science and Sport (Grant No. Z3-6046-0381-04).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kreft, M.E., Sterle, M., Veranič, P. et al. Urothelial injuries and the early wound healing response: tight junctions and urothelial cytodifferentiation. Histochem Cell Biol 123, 529–539 (2005). https://doi.org/10.1007/s00418-005-0770-9
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00418-005-0770-9