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Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology

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Abstract

Chemically-inducible dimerization (CID) is a powerful tool that has proved useful in solving numerous problems in cell biology and related fields. In this review, we focus on case studies where CID was able to provide insight into otherwise refractory problems. Of particular interest are the cases of lipid second messengers and small GTPases, where the “signaling paradox” (how a small pool of signaling molecules can generate a large range of responses) can be at least partly explained through results gleaned from CID experiments. We also discuss several recent technical advances that provide improved specificity in CID action, novel CID substrates that allow simultaneous orthogonal manipulation of multiple systems in one cell, and several applications that move beyond the traditional CID technique of moving a protein of interest to a specific spatiotemporal location.

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Acknowledgments

The authors’ research was supported by NIH grants GM092930 to T.I. We regret that due to length considerations, we could not discuss many worthwhile papers that have appeared in the literature over the years.

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Authors and Affiliations

  1. Department of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA

    Robert DeRose, Takafumi Miyamoto & Takanari Inoue

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  1. Robert DeRose
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  2. Takafumi Miyamoto
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  3. Takanari Inoue
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Correspondence to Takanari Inoue.

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This article is a submission for the special issue on “Recent Advances in Tools for Measuring and Manipulating Biochemical Signals and Mechanical Forces in Living Cells.”

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DeRose, R., Miyamoto, T. & Inoue, T. Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology. Pflugers Arch - Eur J Physiol 465, 409–417 (2013). https://doi.org/10.1007/s00424-012-1208-6

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  • DOI: https://doi.org/10.1007/s00424-012-1208-6

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