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Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity

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Abstract

The Poales (which include the grasses) and Asparagales [which include onion (Allium cepa L.) and other Allium species] are the two most economically important monocot orders. Enormous genomic resources have been developed for the grasses; however, their applicability to other major monocot groups, such as the Asparagales, is unclear. Expressed sequence tags (ESTs) from onion that showed significant similarities (80% similarity over at least 70% of the sequence) to single positions in the rice genome were selected. One hundred new genetic markers developed from these ESTs were added to the intraspecific map derived from the BYG15-23×AC43 segregating family, producing 14 linkage groups encompassing 1,907 cM at LOD 4. Onion linkage groups were assigned to chromosomes using alien addition lines of Allium fistulosum L. carrying single onion chromosomes. Visual comparisons of genetic linkage in onion with physical linkage in rice revealed scant colinearity; however, short regions of colinearity could be identified. Our results demonstrate that the grasses may not be appropriate genomic models for other major monocot groups such as the Asparagales; this will make it necessary to develop genomic resources for these important plants.

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Acknowledgements

This work was completed in compliance with the current laws governing genetic experimentation in Japan, New Zealand, and USA and was supported by U.S. Department of Agriculture, Initiative for Future Agriculture and Food Systems Grant 2001-04434 and Grant-in-Aid for Young Scientists (No. 40314827) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

Disclaimer: Names are necessary to report factually on available data; however, the U.S. Department of Agriculture (USDA) neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

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Authors and Affiliations

  1. Agricultural Research Service, USDA, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI, 53706, USA

    William J. Martin & Michael J. Havey

  2. Crop and Food Research, Private Bag 4704, Christchurch, New Zealand

    John McCallum & Meeghan Pither-Joyce

  3. Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan

    Masayoshi Shigyo & Naoko Yamane

  4. Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI, 53706, USA

    Jernej Jakse

  5. Department of Horticulture, Michigan State University, East Lansing, MI, 48824,  , USA

    Joseph C. Kuhl & Kenneth C. Sink

  6. Horticulture Department, Uludag University, Gorukle, Bursa, 16059, Turkey

    Ali Fuat Gokce

  7. The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD, 20850, USA

    Christopher D. Town

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  1. William J. Martin
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Correspondence to Michael J. Havey.

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Communicated by W.R. McCombie

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Martin, W.J., McCallum, J., Shigyo, M. et al. Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity. Mol Genet Genomics 274, 197–204 (2005). https://doi.org/10.1007/s00438-005-0007-6

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