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VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation

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Abstract

Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).

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Acknowledgements

J.M. is supported by an FWO (‘Fonds voor Wetenschappelijk Onderzoek’) postdoctoral fellowship. R.J. is supported by a grant from the Deutsche Forschungsgemeinschaft (JO369/4-1). We are grateful to Muzahed Uddin Ahmed, Josef Bachmeier, Ulrich Löhren, Christian Prins, and Gert-Jan Zuidam for providing clinical samples from chickens.

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Correspondence to Jelle Matthijnssens.

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Matthijnssens, J., Otto, P.H., Ciarlet, M. et al. VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol 157, 1177–1182 (2012). https://doi.org/10.1007/s00705-012-1273-3

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