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Detection of novel polyomaviruses in fruit bats in Indonesia

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Abstract

Bats are an important natural reservoir for a variety of viral pathogens, including polyomaviruses (PyVs). The aims of this study were: (i) to determine which PyVs are present in bats in Indonesia and (ii) to analyze the evolutionary relationships between bat PyVs and other known PyVs. Using broad-spectrum polymerase chain reaction (PCR)-based assays, we screened PyV DNA isolated from spleen samples from 82 wild fruit bats captured in Indonesia. Fragments of the PyV genome were detected in 10 of the 82 spleen samples screened, and eight full-length viral genome sequences were obtained using an inverse PCR method. A phylogenetic analysis of eight whole viral genome sequences showed that BatPyVs form two distinct genetic clusters within the proposed genus Orthopolyomavirus that are genetically different from previously described BatPyVs. Interestingly, one group of BatPyVs is genetically related to the primate PyVs, including human PyV9 and trichodysplasia spinulosa-associated PyV. This study has identified the presence of novel PyVs in fruit bats in Indonesia and provides genetic information about these BatPyVs.

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Acknowledgments

This study was supported by the Japan Initiative for Global Research Network of Infectious Diseases (J-GRID) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan.

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Authors and Affiliations

  1. Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20 W10, Kita-ku, Sapporo, 001-0020, Japan

    Shintaro Kobayashi, Michihito Sasaki, Yasuko Orba & Hirofumi Sawa

  2. Unit of Risk Analysis and Management, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

    Ryo Nakao

  3. Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia

    Agus Setiyono, Ekowati Handharyani & Mawar Subangkit

  4. Veterinary Investigation and Diagnostic Center, Bukittinggi, Indonesia

    Ibnu Rahmadani

  5. Faculty of Agriculture, Gorontalo State University, Gorontalo, Indonesia

    Siswatiana Taha

  6. Faculty of Animal Husbandry, Sam Ratulangi University, Manado, Indonesia

    Sri Adiani

  7. Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

    Ichiro Nakamura

  8. Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo, 060-0818, Japan

    Takashi Kimura

  9. Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan

    Hirofumi Sawa

  10. Global Virus Network, Baltimore, MD, 21201, USA

    Hirofumi Sawa

Authors
  1. Shintaro Kobayashi
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  2. Michihito Sasaki
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  3. Ryo Nakao
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  4. Agus Setiyono
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  5. Ekowati Handharyani
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  6. Yasuko Orba
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  7. Ibnu Rahmadani
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  8. Siswatiana Taha
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  9. Sri Adiani
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  10. Mawar Subangkit
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  11. Ichiro Nakamura
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  12. Takashi Kimura
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  13. Hirofumi Sawa
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Corresponding authors

Correspondence to Takashi Kimura or Hirofumi Sawa.

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Kobayashi, S., Sasaki, M., Nakao, R. et al. Detection of novel polyomaviruses in fruit bats in Indonesia. Arch Virol 160, 1075–1082 (2015). https://doi.org/10.1007/s00705-015-2349-7

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  • DOI: https://doi.org/10.1007/s00705-015-2349-7

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