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Recombination of baculovirus DNA following lipofection of insect larvae

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Summary

By injection of a liposomal complex containing baculovirus DNA directly into the hemocoel of insect larvae a polyhedrosis disease was induced. After cotransfection of insect larvae with circular viral DNA and transfer vector DNA recombinant viruses were generated with a frequency of about 2%, similar to what is obtained in vitro using insect cell cultures. Based on these results an alternative strategy for the generation of recombinants can be derived for baculoviruses for which susceptible cell lines are not readily available. This strategy involves the injection of baculovirus DNA into susceptible larvae followed by in vivo cloning.

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Authors and Affiliations

  1. Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary, Hungary

    J. P. Hajós, P. Kulcsár & P. Závodszky

  2. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, The Netherlands

    D. Zuidema, J. G. M. Heldens & J. M. Vlak

Authors
  1. J. P. Hajós
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  2. D. Zuidema
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  3. P. Kulcsár
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  4. J. G. M. Heldens
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  5. P. Závodszky
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  6. J. M. Vlak
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Received February 11, 1998 Accepted May 14, 1998

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Hajós, J.P., Zuidema, D., Kulcsár, P. et al. Recombination of baculovirus DNA following lipofection of insect larvae. Arch. Virol. 143, 2045–2050 (1998). https://doi.org/10.1007/s007050050441

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  • DOI: https://doi.org/10.1007/s007050050441

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