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Eye lens proteomics

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An Erratum to this article was published on 24 March 2009

Summary.

The eye lens is a fascinating organ as it is in essence living transparent matter. Lenticular transparency is achieved through the peculiarities of lens morphology, a semi-apoptotic process where cells elongate and loose their organelles and the precise molecular arrangement of the bulk of soluble lenticular proteins, the crystallins. The 16 crystallins ubiquitous in mammals and their modifications have been extensively characterized by 2-DE, liquid chromatography, mass spectrometry and other protein analysis techniques. The various solubility dependant fractions as well as subproteomes of lenticular morphological sections have also been explored in detail. Extensive post translational modification of the crystallins is encountered throughout the lens as a result of ageing and disease resulting in a vast number of protein species. Proteomics methodology is therefore ideal to further comprehensive understanding of this organ and the factors involved in cataractogenesis.

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References

  • HJ Aarts NH Lubsen JG Schoenmakers (1989) ArticleTitleCrystallin gene expression during rat lens development Eur J Biochem 183 31–36 Occurrence Handle2753045 Occurrence Handle1:CAS:528:DyaK3cXhslKjt7k%3D Occurrence Handle10.1111/j.1432-1033.1989.tb14892.x

    Article  PubMed  CAS  Google Scholar 

  • A Abbasi DL Smith JB Smith (1998) ArticleTitleCharacterization of low molecular mass gamma-crystallin fragments from human lenses Exp Eye Res 67 485–488 Occurrence Handle9820797 Occurrence Handle1:CAS:528:DyaK1MXisFah Occurrence Handle10.1006/exer.1998.0564

    Article  PubMed  CAS  Google Scholar 

  • S Abgar J Backmann T Aerts J Vanhoudt J Clauwaert (2000) ArticleTitleThe structural differences between bovine lens alpha A- and alpha B-crystallin Eur J Biochem 267 5916–5925 Occurrence Handle10998051 Occurrence Handle1:CAS:528:DC%2BD3cXnt1Sms7s%3D

    PubMed  CAS  Google Scholar 

  • MS Ajaz Z Ma DL Smith JB Smith (1997) ArticleTitleSize of human lens beta-crystallin aggregates are distinguished by N-terminal truncation of beta B1 J Biol Chem 272 11250–11255 Occurrence Handle9111027 Occurrence Handle1:CAS:528:DyaK2sXjtVamtLg%3D Occurrence Handle10.1074/jbc.272.17.11250

    Article  PubMed  CAS  Google Scholar 

  • FA Asselbergs M Koopmans WJ van Venrooij H Bloemendal (1979) ArticleTitleImproved resolution of calf lens beta-crystallins Exp Eye Res 28 223–228 Occurrence Handle446563 Occurrence Handle1:CAS:528:DyaE1MXhvVamsL0%3D Occurrence Handle10.1016/0014-4835(79)90133-7

    Article  PubMed  CAS  Google Scholar 

  • S Bassnett (2002) ArticleTitleLens organelle degradation Exp Eye Res 74 639–649 Occurrence Handle12076085 Occurrence Handle10.1006/exer.2001.1111 Occurrence Handle1:CAS:528:DC%2BD38XjvFGltbk%3D

    Article  PubMed  CAS  Google Scholar 

  • OA Bateman NH Lubsen C Slingsby (2001) ArticleTitleAssociation behavior of human betaB1-crystallin and its truncated forms Exp Eye Res 73 321–331 Occurrence Handle11520107 Occurrence Handle1:CAS:528:DC%2BD3MXmtFyqsb8%3D Occurrence Handle10.1006/exer.2001.1038

    Article  PubMed  CAS  Google Scholar 

  • OA Bateman R Sarra ST van Genesen G Kappe NH Lubsen C Slingsby (2003) ArticleTitleThe stability of human acidic beta-crystallin oligomers and hetero-oligomers Exp Eye Res 77 409–422 Occurrence Handle12957141 Occurrence Handle1:CAS:528:DC%2BD3sXmvVKmt78%3D Occurrence Handle10.1016/S0014-4835(03)00173-8

    Article  PubMed  CAS  Google Scholar 

  • MJ Bennett S Choe D Eisenberg (1994) ArticleTitleDomain swapping: entangling alliances between proteins Proc Natl Acad Sci USA 91 3127–3131 Occurrence Handle8159715 Occurrence Handle1:CAS:528:DyaK2cXkslKnu78%3D Occurrence Handle10.1073/pnas.91.8.3127

    Article  PubMed  CAS  Google Scholar 

  • MJ Bennett MP Schlunegger D Eisenberg (1995) ArticleTitle3D domain swapping: a mechanism for oligomer assembly Protein Sci 4 2455–2468 Occurrence Handle8580836 Occurrence Handle1:CAS:528:DyaK2MXpslOnsbk%3D

    PubMed  CAS  Google Scholar 

  • GA Berbers OC Boerman H Bloemendal WW de Jong (1982) ArticleTitlePrimary gene products of bovine beta-crystallin and reassociation behavior of its aggregates Eur J Biochem 128 495–502 Occurrence Handle7151791 Occurrence Handle1:CAS:528:DyaL3sXhtlansg%3D%3D Occurrence Handle10.1111/j.1432-1033.1982.tb06992.x

    Article  PubMed  CAS  Google Scholar 

  • GA Berbers WA Hoekman H Bloemendal WW de Jong T Kleinschmidt G Braunitzer (1984) ArticleTitleHomology between the primary structures of the major bovine beta-crystallin chains Eur J Biochem 139 467–479 Occurrence Handle6698025 Occurrence Handle1:CAS:528:DyaL2cXhtlCmsbk%3D Occurrence Handle10.1111/j.1432-1033.1984.tb08029.x

    Article  PubMed  CAS  Google Scholar 

  • SP Bhat (2003) ArticleTitleCrystallins, genes and cataract Prog Drug Res 60 205–262 Occurrence Handle12790344 Occurrence Handle1:CAS:528:DC%2BD3sXlsVemsr8%3D

    PubMed  CAS  Google Scholar 

  • SP Bhat (2004) ArticleTitleTransparency and non-refractive functions of crystallins–a proposal Exp Eye Res 79 809–816 Occurrence Handle15642317 Occurrence Handle1:CAS:528:DC%2BD2MXhvF2juw%3D%3D Occurrence Handle10.1016/j.exer.2004.08.020

    Article  PubMed  CAS  Google Scholar 

  • JG Bindels BM de Man HJ Hoenders (1982) ArticleTitleHigh-performance gel permeation chromatography of bovine eye lens proteins in combination with low-angle laser light scattering. Superior resolution of the oligomeric beta-crystallins J Chromatogr 252 255–267 Occurrence Handle7182411 Occurrence Handle1:CAS:528:DyaL3sXjtVyjsA%3D%3D Occurrence Handle10.1016/S0021-9673(01)88416-8

    Article  PubMed  CAS  Google Scholar 

  • JG Bindels A Koppers HJ Hoenders (1981) ArticleTitleStructural aspects of bovine beta-crystallins: physical characterization including dissociation-association behavior Exp Eye Res 33 333–343 Occurrence Handle7286088 Occurrence Handle1:CAS:528:DyaL3MXlvF2gtrY%3D

    PubMed  CAS  Google Scholar 

  • H Bloemendal (1977) ArticleTitleThe vertebrate eye lens Science 197 127–138 Occurrence Handle877544 Occurrence Handle1:CAS:528:DyaE2sXksl2ks74%3D

    PubMed  CAS  Google Scholar 

  • H Bloemendal W de Jong R Jaenicke NH Lubsen C Slingsby A Tardieu (2004) ArticleTitleAgeing and vision: structure, stability and function of lens crystallins Prog Biophys Mol Biol 86 407–485 Occurrence Handle15302206 Occurrence Handle1:CAS:528:DC%2BD2cXmsVeqsrs%3D Occurrence Handle10.1016/j.pbiomolbio.2003.11.012

    Article  PubMed  CAS  Google Scholar 

  • H Bloemendal WW de Jong (1991) ArticleTitleLens proteins and their genes Prog Nucleic Acid Res Mol Biol 41 259–281 Occurrence Handle1882078 Occurrence Handle1:CAS:528:DyaK3MXmsVyqsr8%3D Occurrence Handle10.1016/S0079-6603(08)60012-4

    Article  PubMed  CAS  Google Scholar 

  • H Bloemendal K Van de Gaer EL Benedetti I Dunia HT Steely (1997) ArticleTitleToward a human crystallin map. Two-dimensional gel electrophoresis and computer analysis of water-soluble crystallins from normal and cataractous human lenses Ophthalmic Res 29 177–190 Occurrence Handle9261842 Occurrence Handle1:STN:280:DyaK2svgsFWksA%3D%3D Occurrence Handle10.1159/000268013

    Article  PubMed  CAS  Google Scholar 

  • J Bours A Wegener D Hofmann HJ Fodisch O Hockwin (1990) ArticleTitleProtein profiles of microsections of the fetal and adult human lens during development and ageing Mech Ageing Dev 54 13–27 Occurrence Handle2195251 Occurrence Handle1:CAS:528:DyaK3cXkslGntbg%3D Occurrence Handle10.1016/0047-6374(90)90012-5

    Article  PubMed  CAS  Google Scholar 

  • MP Bova LL Ding J Horwitz BK Fung (1997) ArticleTitleSubunit exchange of alpha A-crystallin J Biol Chem 272 29511–29517 Occurrence Handle9368012 Occurrence Handle1:CAS:528:DyaK2sXnsFWgur0%3D Occurrence Handle10.1074/jbc.272.47.29511

    Article  PubMed  CAS  Google Scholar 

  • RH Bradley M Ireland H Maisel (1979) ArticleTitleThe cytoskeleton of chick lens cells Exp Eye Res 28 441–453 Occurrence Handle571808 Occurrence Handle1:CAS:528:DyaE1MXktFKqu7k%3D Occurrence Handle10.1016/0014-4835(79)90119-2

    Article  PubMed  CAS  Google Scholar 

  • ML Breitman S Lok G Wistow J Piatigorsky JA Treton RJ Gold LC Tsui (1984) ArticleTitleGamma-crystallin family of the mouse lens: structural and evolutionary relationships Proc Natl Acad Sci USA 81 7762–7766 Occurrence Handle6096855 Occurrence Handle1:CAS:528:DyaL2MXht1Knurk%3D Occurrence Handle10.1073/pnas.81.24.7762

    Article  PubMed  CAS  Google Scholar 

  • HI Calvin JX Wu K Viswanadhan SC Fu (1996) ArticleTitleModifications in lens protein biosynthesis signjal the initiation of cataracts induced by buthionine sulfoximine in mice Exp Eye Res 63 357–368 Occurrence Handle8944543 Occurrence Handle1:CAS:528:DyaK28Xms1Kit74%3D Occurrence Handle10.1006/exer.1996.0126

    Article  PubMed  CAS  Google Scholar 

  • RW Carrell DA Lomas (1997) ArticleTitleConformational disease Lancet 350 134–138 Occurrence Handle9228977 Occurrence Handle1:CAS:528:DyaK2sXkvFeis7s%3D Occurrence Handle10.1016/S0140-6736(97)02073-4

    Article  PubMed  CAS  Google Scholar 

  • JM Carter AM Hutcheson RA Quinlan (1995) ArticleTitleIn vitro studies on the assembly properties of the lens proteins CP49, CP115: coassembly with alpha-crystallin but not with vimentin Exp Eye Res 60 181–192 Occurrence Handle7781747 Occurrence Handle1:CAS:528:DyaK2MXjslehtb0%3D Occurrence Handle10.1016/S0014-4835(95)80009-3

    Article  PubMed  CAS  Google Scholar 

  • JA Carver (1999) ArticleTitleProbing the structure and interactions of crystallin proteins by NMR spectroscopy Prog Retin Eye Res 18 431–462 Occurrence Handle10217479 Occurrence Handle1:CAS:528:DyaK1MXit1Wksbo%3D Occurrence Handle10.1016/S1350-9462(98)00027-5

    Article  PubMed  CAS  Google Scholar 

  • JA Carver PG Cooper RJ Truscott (1993) ArticleTitle1H-NMR spectroscopy of beta B2-crystallin from bovine eye lens Eur J Biochem 213 313–320 Occurrence Handle8477703 Occurrence Handle1:CAS:528:DyaK3sXit1yls7k%3D Occurrence Handle10.1111/j.1432-1033.1993.tb17764.x

    Article  PubMed  CAS  Google Scholar 

  • JA Carver KA Nicholls JA Aquilina RJ Truscott (1996) ArticleTitleAge-related changes in bovine alpha-crystallin and high-molecular-weight protein Exp Eye Res 63 639–647 Occurrence Handle9068371 Occurrence Handle1:CAS:528:DyaK2sXmtVU%3D Occurrence Handle10.1006/exer.1996.0158

    Article  PubMed  CAS  Google Scholar 

  • GJ Caspers JA Leunissen WW de Jong (1995) ArticleTitleThe expanding small heat-shock protein family, and structure predictions of the conserved “alpha-crystallin domain” J Mol Evol 40 238–248 Occurrence Handle7723051 Occurrence Handle1:CAS:528:DyaK2MXksl2gs7o%3D Occurrence Handle10.1007/BF00163229

    Article  PubMed  CAS  Google Scholar 

  • C Chambers P Russell (1991) ArticleTitleDeletion mutation in an eye lens beta-crystallin. An animal model for inherited cataracts J Biol Chem 266 6742–6746 Occurrence Handle1707874 Occurrence Handle1:CAS:528:DyaK38XksFyh

    PubMed  CAS  Google Scholar 

  • SH Chiou P Azari (1989) ArticleTitlePhysicochemical characterization of alpha-crystallins from bovine lenses: hydrodynamic and conformational properties J Protein Chem 8 1–17 Occurrence Handle2765118 Occurrence Handle1:CAS:528:DyaL1MXhsFSjt7o%3D Occurrence Handle10.1007/BF01025075

    Article  PubMed  CAS  Google Scholar 

  • R Clark S Zigman S Lerman (1969) ArticleTitleStudies on the structural proteins of the human lens Exp Eye Res 8 172–182 Occurrence Handle5786867 Occurrence Handle1:CAS:528:DyaF1MXks1yms7g%3D

    PubMed  CAS  Google Scholar 

  • NJ Clout A Basak K Wieligman OA Bateman R Jaenicke C Slingsby (2000) ArticleTitleThe N-terminal domain of betaB2-crystallin resembles the putative ancestral homodimer J Mol Biol 304 253–257 Occurrence Handle11090271 Occurrence Handle1:CAS:528:DC%2BD3cXot1Klt7o%3D Occurrence Handle10.1006/jmbi.2000.4197

    Article  PubMed  CAS  Google Scholar 

  • C Colvis D Garland (2002) ArticleTitlePosttranslational modification of human alpha A-crystallin: correlation with electrophoretic migration Arch Biochem Biophys 397 319–323 Occurrence Handle11795889 Occurrence Handle1:CAS:528:DC%2BD38Xktlymsw%3D%3D Occurrence Handle10.1006/abbi.2001.2669

    Article  PubMed  CAS  Google Scholar 

  • CM Colvis Y Duglas-Tabor KB Werth NE Vieira JA Kowalak A Janjani AL Yergey DL Garland (2000) ArticleTitleTracking pathology with proteomics: identification of in vivo degradation products of alpha B-crystallin Electrophoresis 21 2219–2227 Occurrence Handle10892732 Occurrence Handle1:CAS:528:DC%2BD3cXks1yqsr8%3D Occurrence Handle10.1002/1522-2683(20000601)21:11<2219::AID-ELPS2219>3.0.CO;2-R

    Article  PubMed  CAS  Google Scholar 

  • PG Cooper JA Aquilina RJ Truscott JA Carver (1993a) ArticleTitleSupramolecular order within the lens: 1H NMR spectroscopic evidence for specific crystallin-crystallin interactions Exp Eye Res 59 607–616 Occurrence Handle10.1006/exer.1994.1146

    Article  Google Scholar 

  • PG Cooper JA Carver RJ Truscott (1993b) ArticleTitle1H-NMR spectroscopy of bovine lens beta-crystallin. The role of the beta B2-crystallin C-terminal extension in aggregation Eur J Biochem 213 321–328 Occurrence Handle1:CAS:528:DyaK3sXit1yls7Y%3D Occurrence Handle10.1111/j.1432-1033.1993.tb17765.x

    Article  CAS  Google Scholar 

  • MB Datiles DJ Schumer Js Zigler SuffixJr P Russell L Anderson D Garland (1992) ArticleTitleTwo-dimensional gel electrophoretic analysis of human lens proteins Curr Eye Res 11 669–677 Occurrence Handle1521468 Occurrence Handle1:STN:280:DyaK38zptl2qsw%3D%3D

    PubMed  CAS  Google Scholar 

  • LL David HI Calvin SC Fu (1994) ArticleTitleButhionine sulfoximine induced cataracts in mice contain insolubilized crystallins with calpain II cleavage sites Exp Eye Res 59 501–504 Occurrence Handle7859826 Occurrence Handle1:CAS:528:DyaK2MXitFyhtbc%3D Occurrence Handle10.1006/exer.1994.1136

    Article  PubMed  CAS  Google Scholar 

  • LL David KJ Lampi AL Lund JB Smith (1996) ArticleTitleThe sequence of human betaB1-crystallin cDNA allows mass spectrometric detection of betaB1 protein missing portions of its N-terminal extension J Biol Chem 271 4273–4279 Occurrence Handle8626774 Occurrence Handle1:CAS:528:DyaK28XhtlSmt7w%3D Occurrence Handle10.1074/jbc.271.8.4134

    Article  PubMed  CAS  Google Scholar 

  • LL David TR Shearer (1984) ArticleTitleCalcium-activated proteolysis in the lens nucleus during selenite cataractogenesis Invest Ophthalmol Vis Sci 25 1275–1283 Occurrence Handle6386740 Occurrence Handle1:CAS:528:DyaL2MXit1emuw%3D%3D

    PubMed  CAS  Google Scholar 

  • LL David TR Shearer (1993b) ArticleTitleBeta-crystallin insolubilized by calpain II in vitro contain cleavage sites similar to beta-crystallins insolubilized during cataract FEBS Lett 324 265–270 Occurrence Handle1:STN:280:DyaK2c%2FgvFGqtg%3D%3D Occurrence Handle10.1016/0014-5793(93)80131-D

    Article  CAS  Google Scholar 

  • LL David TR Shearer M Shih (1993a) ArticleTitleSequence analysis of lens beta-crystallins suggests involvement of calpain in cataract formation J Biol Chem 268 1937–1940 Occurrence Handle1:CAS:528:DyaK3sXlslCnsw%3D%3D

    CAS  Google Scholar 

  • AC de Vries MA Vermeer AL Hendriks H Bloemendal LH Cohen (1991) ArticleTitleBiosynthetic capacity of the human lens upon aging Exp Eye Res 53 519–524 Occurrence Handle1936187 Occurrence Handle1:STN:280:DyaK38%2Fjsl2hug%3D%3D Occurrence Handle10.1016/0014-4835(91)90169-F

    Article  PubMed  CAS  Google Scholar 

  • M Delaye A Tardieu (1983) ArticleTitleShort-range order of crystallin proteins accounts for eye lens transparency Nature 302 415–417 Occurrence Handle6835373 Occurrence Handle1:CAS:528:DyaL3sXhslGitrY%3D Occurrence Handle10.1038/302415a0

    Article  PubMed  CAS  Google Scholar 

  • J Dewey C Bartling JL Rae (1995) ArticleTitleA non-enzymatic method for lens decapsulation which leaves the epithelial cells attached to the fibers Curr Eye Res 14 357–362 Occurrence Handle7648861 Occurrence Handle1:STN:280:DyaK2Mzns12ltg%3D%3D

    PubMed  CAS  Google Scholar 

  • M Dole LL Mach RL Hines RC Mobley LD Ferguson MB Alice (1968) ArticleTitleMolecular beams of macroions J Chem Phys 49 2240–2247 Occurrence Handle1:CAS:528:DyaF1cXltVOisLo%3D Occurrence Handle10.1063/1.1670391

    Article  CAS  Google Scholar 

  • HP Driessen P Herbrink H Bloemendal WW de Jong (1981) ArticleTitlePrimary structure of the bovine beta-crystallin Bp chain. Internal duplication and homology with gamma-crystallin Eur J Biochem 121 83–91 Occurrence Handle7035168 Occurrence Handle1:CAS:528:DyaL38XjtVajug%3D%3D Occurrence Handle10.1111/j.1432-1033.1981.tb06433.x

    Article  PubMed  CAS  Google Scholar 

  • P Edman (1949) ArticleTitleA method for the determination of the amino acid sequence in peptides Arch Biochem 22 475–476 Occurrence Handle1:CAS:528:DyaG3cXotV0%3D Occurrence Handle18134557

    CAS  PubMed  Google Scholar 

  • T Emmons L Takemoto (1992) ArticleTitleAge-dependant loss of the C-terminal amino acid from alpha crystallin Exp Eye Res 55 551–554 Occurrence Handle1483501 Occurrence Handle1:CAS:528:DyaK38XmsV2jtLo%3D Occurrence Handle10.1016/S0014-4835(05)80167-8

    Article  PubMed  CAS  Google Scholar 

  • PN Farnsworth B Groth-Vasselli NJ Greenfield K Singh (1997) ArticleTitleEffects of temperature and concentration on bovine lens alpha-crystallin secondary structure: a circular dichroism spectroscopic study Int J Biol Macromol 20 283–291 Occurrence Handle9253648 Occurrence Handle1:CAS:528:DyaK2sXltlOlt74%3D Occurrence Handle10.1016/S0141-8130(97)00028-7

    Article  PubMed  CAS  Google Scholar 

  • J Feng DL Smith JB Smith (2000) ArticleTitleHuman lens beta-crystallin solubility J Biol Chem 275 11585–11590 Occurrence Handle10766773 Occurrence Handle1:CAS:528:DC%2BD3cXislyqurk%3D Occurrence Handle10.1074/jbc.275.16.11585

    Article  PubMed  CAS  Google Scholar 

  • JB Fenn M Mann CK Meng SF Wong CM Whitehouse (1989) ArticleTitleElectrospray ionization for mass spectrometry of large biomolecules Science 246 64–71 Occurrence Handle2675315 Occurrence Handle1:CAS:528:DyaL1MXmtFWhs7w%3D

    PubMed  CAS  Google Scholar 

  • RD Fernald SE Wright (1983) ArticleTitleMaintenance of optical quality during crystalline lens growth Nature 301 618–620 Occurrence Handle6828142 Occurrence Handle1:STN:280:DyaL3s7ktFOmsw%3D%3D Occurrence Handle10.1038/301618a0

    Article  PubMed  CAS  Google Scholar 

  • PG FitzGerald (1988) ArticleTitleAge-related changes in a fiber cell-specific extrinsic membrane protein Curr Eye Res 7 1255–1262 Occurrence Handle3229136 Occurrence Handle1:STN:280:DyaL1M7lt1ehtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • N Fujii M Awakura L Takemoto M Inomata T Takata N Fujii T Saito (2003) ArticleTitleCharacterization of alpha A-crystallin from high molecular weight aggregates in the normal human lens Mol Vis 9 315–322 Occurrence Handle12847419 Occurrence Handle1:CAS:528:DC%2BD3sXntFGnt7s%3D

    PubMed  CAS  Google Scholar 

  • N Fujii S Matsumoto K Hiroki L Takemoto (2001) ArticleTitleInversion and isomerization of Asp-58 residue in human alpha A-crystallin from normal aged lenses and cataractous lenses Biochim Biophys Acta 1549 179–187 Occurrence Handle11690655 Occurrence Handle1:CAS:528:DC%2BD3MXnvFCrs70%3D

    PubMed  CAS  Google Scholar 

  • N Fujii K Satoh K Harada Y Ishibashi (1994) ArticleTitleSimultaneous stereoinversion and isomerization at specific aspartic acid residues in alpha A-crystallin from human lens J Biochem (Tokyo) 116 663–669 Occurrence Handle1:CAS:528:DyaK2MXhtleltbo%3D

    CAS  Google Scholar 

  • N Fujii LJ Takemoto Y Momose S Matsumoto K Hiroki M Akaboshi (1999) ArticleTitleFormation of four isomers at the asp- 151 residue of aged human alpha A-crystallin by natural aging Biochem Biophys Res Commun 265 746–751 Occurrence Handle10600491 Occurrence Handle1:CAS:528:DyaK1MXnvFaqur0%3D Occurrence Handle10.1006/bbrc.1999.1748

    Article  PubMed  CAS  Google Scholar 

  • N Fujii N Takeuchi N Fujii T Tezuka K Kuge T Takata A Kamei T Saito (2004) ArticleTitleComparison of post-translational modifications of alpha A-crystallin from normal and hereditary cataract rats Amino Acids 26 147–152 Occurrence Handle15042443 Occurrence Handle1:CAS:528:DC%2BD2cXkvVyhu7k%3D Occurrence Handle10.1007/s00726-003-0050-8

    Article  PubMed  CAS  Google Scholar 

  • KY Fung S Askovic F Basile MW Duncan (2004) ArticleTitleA simple and inexpensive approach to interfacing high-performance liquid chromatography and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry Proteomics 4 3121–3127 Occurrence Handle15378700 Occurrence Handle1:CAS:528:DC%2BD2cXovVynt70%3D Occurrence Handle10.1002/pmic.200300843

    Article  PubMed  CAS  Google Scholar 

  • R Garadi M Katar H Maisel (1983) ArticleTitleTwo-dimensional gel analysis of chick lens proteins Exp Eye Res 36 859–869 Occurrence Handle6345186 Occurrence Handle1:CAS:528:DyaL3sXksV2iu78%3D Occurrence Handle10.1016/0014-4835(83)90039-8

    Article  PubMed  CAS  Google Scholar 

  • AT Garber D Goring RJ Gold (1984) ArticleTitleCharacterization of abnormal proteins in the soluble lens proteins of CatFraser mice J Biol Chem 259 10376–10379 Occurrence Handle6469970 Occurrence Handle1:CAS:528:DyaL2cXlsV2ht78%3D

    PubMed  CAS  Google Scholar 

  • DL Garland Y Duglas-Tabor J Yimenez-Asensio MB Datiles B Magno (1996) ArticleTitleThe nucleus of the human lens: demonstration of a highly characteristic protein pattern by two-dimensional electrophoresis and introduction of a new method of lens dissection Exp Eye Res 62 285–291 Occurrence Handle8690038 Occurrence Handle1:CAS:528:DyaK28XitFaqsLo%3D Occurrence Handle10.1006/exer.1996.0034

    Article  PubMed  CAS  Google Scholar 

  • B Garner DC Shaw RA Lindner JA Carver RJ Truscott (2000) ArticleTitleNon-oxidative modification of lens crystallins by kynurenine: a novel post-translational protein modification with possible relevance to ageing and cataract Biochim Biophys Acta 1476 265–278 Occurrence Handle10669791 Occurrence Handle1:CAS:528:DC%2BD3cXpsFOrug%3D%3D

    PubMed  CAS  Google Scholar 

  • U Gesierich W Pfeil (1996) ArticleTitleThe conformational stability of alpha-crystallin is rather low: calorimetric results FEBS Lett 393 151–154 Occurrence Handle8814280 Occurrence Handle1:CAS:528:DyaK28Xls1KmtLg%3D Occurrence Handle10.1016/0014-5793(96)00867-8

    Article  PubMed  CAS  Google Scholar 

  • X Gong E Li G Klier Q Huang Y Wu H Lei NM Kumar J Horwitz NB Gilula (1997) ArticleTitleDisruption of alpha3 connexin gene leads to proteolysis and cataractogenesis in mice Cell 91 833–843 Occurrence Handle9413992 Occurrence Handle1:CAS:528:DyaK2sXotV2itbc%3D Occurrence Handle10.1016/S0092-8674(00)80471-7

    Article  PubMed  CAS  Google Scholar 

  • DA Goodenough (1992) ArticleTitleThe crystalline lens. A system networked by gap junctional intercellular communication Semin Cell Biol 3 49–58 Occurrence Handle1320431 Occurrence Handle1:STN:280:DyaK38zit12qug%3D%3D Occurrence Handle10.1016/S1043-4682(10)80007-8

    Article  PubMed  CAS  Google Scholar 

  • DR Goring ML Breitman LC Tsui (1992) ArticleTitleTemporal regulation of six crystallin transcripts during mouse lens development Exp Eye Res 54 785–795 Occurrence Handle1623964 Occurrence Handle1:CAS:528:DyaK38XltVyktLY%3D Occurrence Handle10.1016/0014-4835(92)90034-P

    Article  PubMed  CAS  Google Scholar 

  • J Graw (1997) ArticleTitleThe crystallins: genes, proteins and diseases Biol Chem 278 1331–1348

    Google Scholar 

  • J Graw (2003) ArticleTitleThe genetic and molecular basis of congenital eye defects Nat Rev Genet 4 876–888 Occurrence Handle14634635 Occurrence Handle1:CAS:528:DC%2BD3sXptFaht70%3D Occurrence Handle10.1038/nrg1202

    Article  PubMed  CAS  Google Scholar 

  • J Graw A Liebstein D Pietrowski T Schmitt-John T Werner (1993) ArticleTitleGenomic sequences of murine gamma B- and gamma C-crystallin-encoding genes: promoter analysis and complete evolutionary pattern of mouse, rat and human gamma-crystallins Gene 136 145–156 Occurrence Handle8293998 Occurrence Handle1:CAS:528:DyaK2cXjtFKqsbk%3D Occurrence Handle10.1016/0378-1119(93)90458-F

    Article  PubMed  CAS  Google Scholar 

  • PJ Groenen KB Merck WW de Jong H Bloemendal (1994) ArticleTitleStructure and modifications of the junior chaperone alpha-crystallin. From lens transparency to molecular pathology Eur J Biochem 225 1–19 Occurrence Handle7925426 Occurrence Handle1:CAS:528:DyaK2cXlvFylsLY%3D Occurrence Handle10.1111/j.1432-1033.1994.00001.x

    Article  PubMed  CAS  Google Scholar 

  • DA Haley J Horwitz PL Stewart (1998) ArticleTitleThe small heat-shock protein, alpha B-crystallin, has a variable quaternary structure J Mol Biol 277 27–35 Occurrence Handle9514758 Occurrence Handle1:CAS:528:DyaK1cXisFGjurY%3D Occurrence Handle10.1006/jmbi.1997.1611

    Article  PubMed  CAS  Google Scholar 

  • SR Hanson A Hasan DL Smith JB Smith (2000) ArticleTitleThe major in vivo modifications of the human water-insoluble lens crystallins are disulfide bonds, deamidation, methionine oxidation and backbone cleavage Exp Eye Res 71 195–207 Occurrence Handle10930324 Occurrence Handle1:CAS:528:DC%2BD3cXltlOqtL8%3D Occurrence Handle10.1006/exer.2000.0868

    Article  PubMed  CAS  Google Scholar 

  • SR Hanson DL Smith JB Smith (1998) ArticleTitleDeamidation and disulfide bonding in human lens gamma-crystallins Exp Eye Res 67 301–312 Occurrence Handle9778411 Occurrence Handle1:CAS:528:DyaK1cXntFWnsbc%3D Occurrence Handle10.1006/exer.1998.0530

    Article  PubMed  CAS  Google Scholar 

  • JJ Harding (1972) ArticleTitleConformational changes in human lens proteins in cataract Biochem J 129 97–100 Occurrence Handle4646781 Occurrence Handle1:CAS:528:DyaE38Xls1aktL0%3D

    PubMed  CAS  Google Scholar 

  • JJ Harding (2002) ArticleTitleViewing molecular mechanisms of ageing through a lens Ageing Res Rev 1 465–479 Occurrence Handle12067598 Occurrence Handle1:CAS:528:DC%2BD38XpsFKhsrg%3D Occurrence Handle10.1016/S1568-1637(02)00012-0

    Article  PubMed  CAS  Google Scholar 

  • V Harrington S McCall S Huynh K Srivastava OP Srivastava (2004) ArticleTitleCrystallins in water soluble-high molecular weight protein fractions and water insoluble protein fractions in aging and cataractous human lenses Mol Vis 10 476–489 Occurrence Handle15303090 Occurrence Handle1:CAS:528:DC%2BD2cXntFOksL4%3D

    PubMed  CAS  Google Scholar 

  • A Hasan DL Smith JB Smith (2002) ArticleTitleAlpha-crystallin regions affected by adenosine 5′-triphosphate identified by hydrogen-deuterium exchange Biochemistry 41 15875–15882 Occurrence Handle10.1021/bi026568x Occurrence Handle1:CAS:528:DC%2BD38Xpt1aisrw%3D

    Article  CAS  Google Scholar 

  • JF Hejtmancik PT Wingfield C Chambers P Russel HC Chen YV Sergeev JN Hope (1997) ArticleTitleAssociation properties of betaB2- and betaA3-crystallin: ability to form dimers Protein Eng 10 1347–1352 Occurrence Handle9514125 Occurrence Handle1:CAS:528:DyaK1cXhs1ylsro%3D Occurrence Handle10.1093/protein/10.11.1347

    Article  PubMed  CAS  Google Scholar 

  • JF Hejtmancik PT Wingfield YV Sergeev (2004) ArticleTitleBeta-crystallin association Exp Eye Res 79 377–383 Occurrence Handle1:CAS:528:DC%2BD2cXntFCjs7s%3D Occurrence Handle10.1016/j.exer.2004.06.011

    Article  CAS  Google Scholar 

  • W Hendriks H Weetnik CE Voorter J Sanders H Bloemendal WW de Jong (1990) ArticleTitleThe alternative splicing product alpha Ains-crystallin is structurally equivalent to alpha A and alpha B subunits in the rat alpha crystallin aggregate Biochim Biophys Acta 1037 58–65 Occurrence Handle2294971 Occurrence Handle1:CAS:528:DyaK3cXkvFensbY%3D

    PubMed  CAS  Google Scholar 

  • Hoehenwarter W, Ackermann R, Zimny-Arndt U, Kumar NM, Jungblut PR (2006) The necessity of functional proteomics: protein species and molecular function elucidation exemplified by in vivo alpha A crystallin N-terminal truncation. Amino Acids (in press)

  • W Hoehenwarter NM Kumar M Wacker U Zimny-Arndt J Klose PR Jungblut (2005) ArticleTitleEye lens proteomics: from global approach to detailed information about phakinin and gamma E and F crystallin genes Proteomics 5 245–257 Occurrence Handle15744838 Occurrence Handle1:CAS:528:DC%2BD2MXhtleisLw%3D Occurrence Handle10.1002/pmic.200300878

    Article  PubMed  CAS  Google Scholar 

  • J Horwitz (1992) ArticleTitleAlpha-crystallin can function as a molecular chaperone Proc Natl Acad Sci USA 89 10449–10453 Occurrence Handle1438232 Occurrence Handle1:CAS:528:DyaK3sXisFCm Occurrence Handle10.1073/pnas.89.21.10449

    Article  PubMed  CAS  Google Scholar 

  • J Horwitz QL Huang L Ding MP Bova (1998) ArticleTitleLens alpha-crystallin: chaperone like properties Methods Enzymol 290 365–383 Occurrence Handle9534176 Occurrence Handle1:CAS:528:DyaK1cXis1GhurY%3D Occurrence Handle10.1016/S0076-6879(98)90032-5

    Article  PubMed  CAS  Google Scholar 

  • M Ireland H Maisel (1984) ArticleTitleA cytoskeltetal protein unique to lens fiber cell differentiation Exp Eye Res 38 637–645 Occurrence Handle6381079 Occurrence Handle1:CAS:528:DyaL2cXlsVylt7c%3D Occurrence Handle10.1016/0014-4835(84)90182-9

    Article  PubMed  CAS  Google Scholar 

  • M Ireland H Maisel (1989) ArticleTitleA family of lens fiber cell specific proteins Lens Eye Toxic Res 6 623–638 Occurrence Handle2487275 Occurrence Handle1:CAS:528:DyaK3cXhtFKhsr0%3D

    PubMed  CAS  Google Scholar 

  • T Iwaki A Kume-Iwaki JE Goldman (1990) ArticleTitleCellular distribution of alpha B-crystallin in non-lenticular tissues J Histochem Cytochem 38 31–39 Occurrence Handle2294148 Occurrence Handle1:CAS:528:DyaK3cXnsVelsQ%3D%3D

    PubMed  CAS  Google Scholar 

  • JA Jedziniak DF Nicoli H Baram GB Benedek (1978) ArticleTitleQuantative verification of the existence of high molecular weight protein aggregates in the intact normal human lens by light-scattering spectroscopy Invest Ophthalmol Vis Sci 17 51–57 Occurrence Handle621125 Occurrence Handle1:CAS:528:DyaE1cXos1OmsQ%3D%3D

    PubMed  CAS  Google Scholar 

  • J Jimenez-Asensio CM Colvis JA Kowalak Y Duglas-Tabor MB Datiles M Moroni U Mura CM Rao D Balasubramanian A Janjani D Garland (1999) ArticleTitleAn atypical form of alpha B-crystallin is present in high concentration in some human cataractous lenses. Identification and characterization of aberrant N- and C-terminal processing J Biol Chem 274 32287–32294 Occurrence Handle10542268 Occurrence Handle1:CAS:528:DyaK1MXnt12jurw%3D Occurrence Handle10.1074/jbc.274.45.32287

    Article  PubMed  CAS  Google Scholar 

  • P Jungblut J Klose (1985) ArticleTitleGenetic variability of proteins from mitochondria and mitochondrial fractions of mouse organs Biochem Genet 23 227–245 Occurrence Handle4015616 Occurrence Handle1:CAS:528:DyaL2MXkt1Sgt7k%3D Occurrence Handle10.1007/BF00504321

    Article  PubMed  CAS  Google Scholar 

  • P Jungblut B Thiede (1997) ArticleTitleProtein identification from 2-DE gels by MALDI mass spectrometry Mass Spectrom Rev 16 145–162 Occurrence Handle9414492 Occurrence Handle1:CAS:528:DyaK2sXnvFOhsL8%3D Occurrence Handle10.1002/(SICI)1098-2787(1997)16:3<145::AID-MAS2>3.0.CO;2-H

    Article  PubMed  CAS  Google Scholar 

  • P Jungblut B Thiede U Zimny-Arndt EC Müller C Scheler B Wittmann-Liebold A Otto (1996) ArticleTitleResolution power of two-dimensional electrophoresis and identification of proteins from gels Electrophoresis 17 839–847 Occurrence Handle8783010 Occurrence Handle1:CAS:528:DyaK28Xjtlylt7c%3D Occurrence Handle10.1002/elps.1150170505

    Article  PubMed  CAS  Google Scholar 

  • PR Jungblut A Otto J Favor M Lowe EC Muller M Kastnetr K Sperling J Klose (1998) ArticleTitleIdentification of mouse crystallins in 2D protein patterns by sequencing and mass spectrometry. Application to cataract mutants FEBS Lett 435 131–137 Occurrence Handle9762894 Occurrence Handle1:CAS:528:DyaK1cXmt1emt7w%3D Occurrence Handle10.1016/S0014-5793(98)01053-9

    Article  PubMed  CAS  Google Scholar 

  • A Kamei H Iwase K Masuda (1997) ArticleTitleCleavage of amino acid residue(s) from the N-terminal region of alpha A- and alpha B-crystallins in human crystalline lens during aging Biochem Biophys Res Commun 231 373–378 Occurrence Handle9070282 Occurrence Handle1:CAS:528:DyaK2sXhsVegt7s%3D Occurrence Handle10.1006/bbrc.1997.6105

    Article  PubMed  CAS  Google Scholar 

  • A Kamei N Takeuchi M Nagai S Mori (2003) ArticleTitlePost-translational modification of beta H-crystallin of bovine lens with aging Biol Pharm Bull 26 1715–1720 Occurrence Handle14646177 Occurrence Handle1:CAS:528:DC%2BD3sXpvFaks7s%3D Occurrence Handle10.1248/bpb.26.1715

    Article  PubMed  CAS  Google Scholar 

  • M Karas F Hillenkamp (1988) ArticleTitleLaser desorption ionization of proteins with molecular masses exceeding 10,000 daltons Anal Chem 60 2299–2301 Occurrence Handle3239801 Occurrence Handle1:CAS:528:DyaL1cXlsVGmu7g%3D Occurrence Handle10.1021/ac00171a028

    Article  PubMed  CAS  Google Scholar 

  • MJ Kelley LL David N Iwasaki J Wright TR Shearer (1993) ArticleTitlealpha-Crystallin chaperone activity is reduced by calpain II in vitro and in selenite cataract J Biol Chem 268 18844–18849 Occurrence Handle8395520 Occurrence Handle1:CAS:528:DyaK3sXmtVWisL8%3D

    PubMed  CAS  Google Scholar 

  • M Kibbelaar H Bloemendal (1975) ArticleTitleThe topography of lens proteins based on chromatography and two-dimensional gel electrophoresis Exp Eye Res 21 25–36 Occurrence Handle1170083 Occurrence Handle1:CAS:528:DyaE2MXkvFalsLk%3D Occurrence Handle10.1016/0014-4835(75)90054-8

    Article  PubMed  CAS  Google Scholar 

  • MA Kibbelaar H Bloemendal (1979) ArticleTitleFractionation of the water-insoluble proteins from calf lens Exp Eye Res 29 679–688 Occurrence Handle544284 Occurrence Handle1:CAS:528:DyaL3cXhtVKisLk%3D Occurrence Handle10.1016/0014-4835(79)90024-1

    Article  PubMed  CAS  Google Scholar 

  • Kilby GW (1995a) Electrospray mass spectrometry of peptides and lens proteins. Ph.D. Thesis, The University of Wollongong, Australia

  • GW Kilby JA Carver JL Zhu MM Sheil RJ Truscott (1995b) ArticleTitleLoss of the C-terminal serine residue from bovine beta B2-crystallin Exp Eye Res 60 465–469 Occurrence Handle1:CAS:528:DyaK2MXlvVOqsb0%3D Occurrence Handle10.1016/S0014-4835(05)80061-2

    Article  CAS  Google Scholar 

  • GW Kilby MM Sheil D Shaw JJ Harding RJ Truscott (1997) ArticleTitleAmino acid sequence of bovine gamma E (IVa) lens crystallin Protein Sci 6 909–912 Occurrence Handle9098901 Occurrence Handle1:CAS:528:DyaK2sXisFWmu70%3D

    PubMed  CAS  Google Scholar 

  • NJ Kleiman R Chiesa MA Kolks A Spector (1988) ArticleTitlePhosphorylation of beta-crystallin B2 (beta Bp) in the bovine lens J Biol Chem 263 14978–14983 Occurrence Handle3170571 Occurrence Handle1:CAS:528:DyaL1cXlvVOrtbk%3D

    PubMed  CAS  Google Scholar 

  • R Klemenz E Frohli RH Steiger R Schafer A Aoyama (1991) ArticleTitleAlpha B-crystallin is a small heat shock protein Proc Natl Acad Sci USA 88 3652–3655 Occurrence Handle2023914 Occurrence Handle1:CAS:528:DyaK3MXktVenurg%3D Occurrence Handle10.1073/pnas.88.9.3652

    Article  PubMed  CAS  Google Scholar 

  • J Klose (1975) ArticleTitleProtein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals Humangenetik 26 231–243 Occurrence Handle1093965 Occurrence Handle1:CAS:528:DyaE28Xltl2gtro%3D

    PubMed  CAS  Google Scholar 

  • J Klose U Kobalz (1995) ArticleTitleTwo-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome Electrophoresis 16 1034–1059 Occurrence Handle7498127 Occurrence Handle1:CAS:528:DyaK2MXmslKmurg%3D Occurrence Handle10.1002/elps.11501601175

    Article  PubMed  CAS  Google Scholar 

  • J Klose C Nock M Herrmann K Stühler K Marcus M Blüggel E Krause LC Schalwyk S Rastan SDM Brown K Büssow H Himmelbauer H Lehrach (2002) ArticleTitleGenetic analysis of the mouse brain proteome Nat Genet 30 385–393 Occurrence Handle11912495 Occurrence Handle1:CAS:528:DC%2BD38Xisl2lsLs%3D Occurrence Handle10.1038/ng861

    Article  PubMed  CAS  Google Scholar 

  • JF Koretz GH Handelman (1988) ArticleTitleHow the human eye focuses Sci Am 259 92–99 Occurrence Handle3070746 Occurrence Handle1:STN:280:DyaL1M7os1yjuw%3D%3D Occurrence Handle10.1038/scientificamerican0788-92

    Article  PubMed  CAS  Google Scholar 

  • A Krah S Miehlke KP Pleissner U Zimny-Arndt C Kirsch N Lehn TF Meyer PR Jungblut T Aebischer (2004) ArticleTitleIdentification of candidate antigens for serologic detection of Helicobacter pylori-infected patients with gastric carcinoma Int J Cancer 108 456–463 Occurrence Handle14648714 Occurrence Handle1:CAS:528:DC%2BD3sXhtVSlsr%2FM Occurrence Handle10.1002/ijc.11557

    Article  PubMed  CAS  Google Scholar 

  • RH Kröger MC Campbell R Munger RD Fernald (1994) ArticleTitleRefractive index distribution and spherical aberration in the crystalline lens of the African cichlid fish Haplochromis burtoni Vis Res 34 1815–1822 Occurrence Handle7941384 Occurrence Handle10.1016/0042-6989(94)90306-9

    Article  PubMed  Google Scholar 

  • JR Kuszak KL Peterson JG Sivak KL Herbert (1994) ArticleTitleThe interrelationship of lens anatomy and optical quality. II. Primate lenses Exp Eye Res 59 521–535 Occurrence Handle9492754 Occurrence Handle1:CAS:528:DyaK2MXisVGkurs%3D Occurrence Handle10.1006/exer.1994.1138

    Article  PubMed  CAS  Google Scholar 

  • T Kuwabara (1975) ArticleTitleThe maturation of the lens cell: a morphologic study Exp Eye Res 20 427–443 Occurrence Handle1126408 Occurrence Handle1:STN:280:DyaE2M7kvFOhtg%3D%3D Occurrence Handle10.1016/0014-4835(75)90085-8

    Article  PubMed  CAS  Google Scholar 

  • S Lambert (1994) Lens D Albert F Jakobiec (Eds) Principles and practices of ophthalmology Saunders Philadelphia

    Google Scholar 

  • K Lampi M Shih Y Ueda TR Shearer LL David (2002) ArticleTitleLens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map Invest Ophthalmol Vis Sci 43 216–224 Occurrence Handle11773034

    PubMed  Google Scholar 

  • KJ Lampi Z Ma SR Hanson M Azuma M Shih TR Shearer DL Smith JB Smith LL David (1998) ArticleTitleAge-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry Exp Eye Res 67 31–43 Occurrence Handle9702176 Occurrence Handle1:CAS:528:DyaK1cXltVentb0%3D Occurrence Handle10.1006/exer.1998.0481

    Article  PubMed  CAS  Google Scholar 

  • KJ Lampi Z Ma M Shih TR Shearer JB Smith DL Smith LL David (1997) ArticleTitleSequence analysis of betaA3, betaB3, and betaA4 crystallins completes the identification of the major proteins in young human lens J Biol Chem 272 2268–2275 Occurrence Handle8999933 Occurrence Handle1:CAS:528:DyaK2sXotV2ltw%3D%3D Occurrence Handle10.1074/jbc.272.4.2268

    Article  PubMed  CAS  Google Scholar 

  • KJ Lampi JT Oxford HP Bachinger TR Shearer LL David DM Kapfer (2001) ArticleTitleDeamidation of human beta B1 alters the elongated structure of the dimer Exp Eye Res 72 279–288 Occurrence Handle11180977 Occurrence Handle1:CAS:528:DC%2BD3MXht1aqs78%3D Occurrence Handle10.1006/exer.2000.0950

    Article  PubMed  CAS  Google Scholar 

  • VN Lapko AG Pukiss DL Smith JB Smith (2002) ArticleTitleDeamidation in human gamma S-crystallin from cataractous lenses is influenced by surface exposure Biochemistry 41 8638–8648 Occurrence Handle12093281 Occurrence Handle1:CAS:528:DC%2BD38Xkt1Clsr4%3D Occurrence Handle10.1021/bi015924t

    Article  PubMed  CAS  Google Scholar 

  • VN Lapko DL Smith JB Smith (2001) ArticleTitleIn vivo carbamylation and acetylation of water-soluble human lens alpha B-crystallin lysine 92 Protein Sci 10 1130–1136 Occurrence Handle11369851 Occurrence Handle1:CAS:528:DC%2BD3MXksFCjsro%3D Occurrence Handle10.1110/ps.40901

    Article  PubMed  CAS  Google Scholar 

  • VN Lapko DL Smith JB Smith (2003a) ArticleTitleExpression of beta A2 crystallin in human lenses Exp Eye Res 77 383–385 Occurrence Handle1:CAS:528:DC%2BD3sXlvFGgt74%3D Occurrence Handle10.1016/S0014-4835(03)00156-8

    Article  CAS  Google Scholar 

  • VN Lapko DL Smith JB Smith (2003b) ArticleTitleMethylation and carbamylation of human gamma-crystallins Protein Sci 12 1762–1774 Occurrence Handle1:CAS:528:DC%2BD3sXlvFSjurk%3D Occurrence Handle10.1110/ps.0305403

    Article  CAS  Google Scholar 

  • LK Li D Roy A Spector (1986) ArticleTitleChanges in lens protein in concentric fractions from individual normal human lenses Curr Eye Res 5 127–135 Occurrence Handle3956240 Occurrence Handle1:CAS:528:DyaL1cXhs1Ghsbo%3D

    PubMed  CAS  Google Scholar 

  • W Li HI Calvin LL David K Wu AL McCormack GP Zhu SC Fu (2002) ArticleTitleAltered patterns of phosphorylation in cultured mouse lenses during development of buthionine sulfoximine cataracts Exp Eye Res 75 335–346 Occurrence Handle12384096 Occurrence Handle1:CAS:528:DC%2BD38Xns1GmsLc%3D

    PubMed  CAS  Google Scholar 

  • P Lin DL Smith JB Smith (1997) ArticleTitleIn vivo modification of the C-terminal lysine of human lens alpha B-crystallin Exp Eye Res 65 673–680 Occurrence Handle9367647 Occurrence Handle1:CAS:528:DyaK2sXnsFWlsbw%3D Occurrence Handle10.1006/exer.1997.0376

    Article  PubMed  CAS  Google Scholar 

  • PP Lin RC Barry DL Smith JB Smith (1998) ArticleTitleIn vivo acetylation identified at lysine 70 of human lens alpha A-crystallin Protein Sci 7 1451–1457 Occurrence Handle9655350 Occurrence Handle1:CAS:528:DyaK1cXjvFGqsLw%3D

    PubMed  CAS  Google Scholar 

  • AJ Link J Eng DM Schieltz E Carmack GJ Mize DR Morris BM Garvik JR Yates Suffix3rd (1999) ArticleTitleDirect analysis of protein complexes using mass spectrometry Nat Biotechnol 17 676–682 Occurrence Handle10404161 Occurrence Handle1:CAS:528:DyaK1MXksFehsLc%3D Occurrence Handle10.1038/10890

    Article  PubMed  CAS  Google Scholar 

  • S Lok LC Tsui T Shinohara J Piatigorsky R Gold M Breitman (1984) ArticleTitleAnalysis of the mouse gamma-crystallin gene family: assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene Nucleic Acids Res 12 4517–4529 Occurrence Handle6330674 Occurrence Handle1:CAS:528:DyaL2cXlsVGjsrg%3D

    PubMed  CAS  Google Scholar 

  • NH Lubsen HJ Aarts JG Schoenmakers (1988) ArticleTitleThe evolution of lenticular proteins: the beta- and gamma-crystallin super gene family Prog Biophys Mol Biol 51 47–76 Occurrence Handle3064189 Occurrence Handle1:CAS:528:DyaL1MXovFCksA%3D%3D Occurrence Handle10.1016/0079-6107(88)90010-7

    Article  PubMed  CAS  Google Scholar 

  • AL Lund JB Smith DL Smith (1996) ArticleTitleModifications of the water-insoluble human lens alpha-crystallins Exp Eye Res 63 661–672 Occurrence Handle9068373 Occurrence Handle1:CAS:528:DyaK2sXmtVw%3D Occurrence Handle10.1006/exer.1996.0160

    Article  PubMed  CAS  Google Scholar 

  • Z Ma SR Hanson KJ Lampi LL David DL Smith JB Smith (1998) ArticleTitleAge-related changes in human lens crystallins identified by HPLC and mass spectrometry Exp Eye Res 67 21–30 Occurrence Handle9702175 Occurrence Handle1:CAS:528:DyaK1cXltVentLg%3D Occurrence Handle10.1006/exer.1998.0482

    Article  PubMed  CAS  Google Scholar 

  • MJ MacCoss WH McDonald A Saraf R Sadygov JM Clark JJ Tasto KL Gould D Wolters M Washburn A Weiss JI Clark JR Yates Suffix3rd (2002) ArticleTitleShotgun identification of protein modifications from protein complexes and lens tissue Proc Natl Acad Sci USA 99 7900–7905 Occurrence Handle12060738 Occurrence Handle1:CAS:528:DC%2BD38XkvVGjsbg%3D Occurrence Handle10.1073/pnas.122231399

    Article  PubMed  CAS  Google Scholar 

  • H Maisel MM Perry (1972) ArticleTitleElectron microscope observations on some structural proteins of the chick lens Exp Eye Res 14 7–12 Occurrence Handle4114289 Occurrence Handle1:STN:280:DyaE383isFahtw%3D%3D Occurrence Handle10.1016/0014-4835(72)90136-4

    Article  PubMed  CAS  Google Scholar 

  • M Mann RC Hendrickson A Pandey (2001) ArticleTitleAnalysis of proteins and proteomes by mass spectrometry Annu Rev Biochem 70 437–473 Occurrence Handle11395414 Occurrence Handle1:CAS:528:DC%2BD3MXlsVehtL4%3D Occurrence Handle10.1146/annurev.biochem.70.1.437

    Article  PubMed  CAS  Google Scholar 

  • RT Mathias JL Rae GJ Baldo (1997) ArticleTitlePhysiological properties of the normal lens Physiol Rev 77 21–50 Occurrence Handle9016299 Occurrence Handle1:CAS:528:DyaK2sXhvVeqsrk%3D

    PubMed  CAS  Google Scholar 

  • AL McCormack DM Schieltz B Goode S Yang G Barnes D Drubin JR Yates Suffix3rd (1997) ArticleTitleDirect analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level Anal Chem 69 767–776 Occurrence Handle9043199 Occurrence Handle1:CAS:528:DyaK2sXhtVCqtLs%3D Occurrence Handle10.1021/ac960799q

    Article  PubMed  CAS  Google Scholar 

  • M Messmer B Chakrabarti (1988) ArticleTitleHigh-molecular-weight protein aggregates of calf and cow lens: spectroscopic evaluation Exp Eye Res 47 173–183 Occurrence Handle3409989 Occurrence Handle1:CAS:528:DyaL1cXkvFShur4%3D Occurrence Handle10.1016/0014-4835(88)90001-2

    Article  PubMed  CAS  Google Scholar 

  • LR Miesbauer X Zhou Z Yang Z Yang Y Sun DL Smith JB Smith (1994) ArticleTitlePost-translational modifications of water-soluble human lens crystallins from young adults J Biol Chem 269 12494–12502 Occurrence Handle8175657 Occurrence Handle1:CAS:528:DyaK2cXjtFyjsL8%3D

    PubMed  CAS  Google Scholar 

  • DC Minassian V Mehra (1990) ArticleTitle3.8 million blinded by cataract each year: projections from the first epidemiological study of incidence of cataract blindness in India Br J Ophthalmol 74 341–343 Occurrence Handle2378840 Occurrence Handle1:STN:280:DyaK3czjsFWmtA%3D%3D

    PubMed  CAS  Google Scholar 

  • CT Mörner (1894) ArticleTitleUntersuchung der Proteinsubstanzen in den leichtbrechenden Medien des Auges Z Physiol Chem 18 61–106

    Google Scholar 

  • E Nagele M Vollmer (2004) ArticleTitleCoupling of nanoflow liquid chromatography to matrix-assisted laser desorption/ionization mass spectrometry: real-time liquid chromatography run mapping on a MALDI plate Rapid Commun Mass Spectrom 18 3008–3014 Occurrence Handle15536631 Occurrence Handle10.1002/rcm.1723 Occurrence Handle1:CAS:528:DC%2BD2cXhtFarsbvN

    Article  PubMed  CAS  Google Scholar 

  • BV Norledge EM Mayr R Glockshuber OA Bateman C Slingsby R Jaenicke HP Driessen (1996) ArticleTitleThe X-ray structures of two mutant crystallin domains shed light on the evolution of multi-domain proteins Nat Struct Biol 3 267–274 Occurrence Handle8605629 Occurrence Handle1:CAS:528:DyaK28XhslSlsbY%3D Occurrence Handle10.1038/nsb0396-267

    Article  PubMed  CAS  Google Scholar 

  • PH O’Farrell (1975) ArticleTitleHigh resolution two-dimensional electrophoresis of proteins J Biol Chem 250 4007–4021 Occurrence Handle236308 Occurrence Handle1:CAS:528:DyaE2MXktl2ltro%3D

    PubMed  CAS  Google Scholar 

  • BJ Ortwerth PR Olesen KK Sharma (1986) ArticleTitleSolubilization of the lens water-insoluble fraction by sonication Exp Eye Res 43 955–963 Occurrence Handle3817033 Occurrence Handle1:CAS:528:DyaL2sXhtlCks7Y%3D Occurrence Handle10.1016/0014-4835(86)90073-4

    Article  PubMed  CAS  Google Scholar 

  • BJ Ortwerth PR Olesen (1989) ArticleTitleStudies on the nature of the water-insoluble fraction from aged bovine lenses Exp Eye Res 48 605–619 Occurrence Handle2737259 Occurrence Handle1:CAS:528:DyaL1MXktVCrtbY%3D Occurrence Handle10.1016/0014-4835(89)90003-1

    Article  PubMed  CAS  Google Scholar 

  • BJ Ortwerth PR Olesen (1992) ArticleTitleStudies on the solubilization of the water-insoluble fraction from human lens and cataract Exp Eye Res 55 777–783 Occurrence Handle1486936 Occurrence Handle1:CAS:528:DyaK3sXnvVGltA%3D%3D Occurrence Handle10.1016/0014-4835(92)90004-C

    Article  PubMed  CAS  Google Scholar 

  • J Papaconstantinou (1967) ArticleTitleMolecular aspects of lens cell differentiation Science 156 338–346 Occurrence Handle4886533 Occurrence Handle1:CAS:528:DyaF2sXktFOktbw%3D

    PubMed  CAS  Google Scholar 

  • I Paron A D’Elia C D’Ambrosio A Scaloni F D’Aurizio A Prescott G Damante G Tell (2004) ArticleTitleA proteomic approach to identify early molecular targets of oxidative stress in human epithelial lens cells Biochem J 378 929–937 Occurrence Handle14678012 Occurrence Handle1:CAS:528:DC%2BD2cXisVyktbw%3D Occurrence Handle10.1042/BJ20031190

    Article  PubMed  CAS  Google Scholar 

  • SY Pasta B Raman T Ramakrishna ChM Rao (2003) ArticleTitleRole of the conserved SRLFDQFFG region of alpha-crystallin, a small heat shock protein. Effect on oligomeric size, subunit exchange and chaperone-like activity J Biol Chem 278 51159–51166 Occurrence Handle14532291 Occurrence Handle1:CAS:528:DC%2BD3sXpslOhsb8%3D Occurrence Handle10.1074/jbc.M307523200

    Article  PubMed  CAS  Google Scholar 

  • N Puri RC Augusteyn EA Owen RJ Siezen (1983) ArticleTitleImmunochemical properties of vertebrate alpha-crystallins Eur J Biochem 134 321–326 Occurrence Handle6191981 Occurrence Handle1:CAS:528:DyaL3sXkvVKhu7k%3D Occurrence Handle10.1111/j.1432-1033.1983.tb07569.x

    Article  PubMed  CAS  Google Scholar 

  • JL Rae C Bartling J Rae RT Mathias (1996) ArticleTitleDye transfer between cells of the lens J Membr Biol 150 89–103 Occurrence Handle8699483 Occurrence Handle1:CAS:528:DyaK28XhslCmtbc%3D Occurrence Handle10.1007/s002329900033

    Article  PubMed  CAS  Google Scholar 

  • FC Ramaekers TR Boomkens H Bloemendal (1981) ArticleTitleCytoskeletal and contractile structures in bovine lens cell differentiation Exp Cell Res 35 454–461 Occurrence Handle10.1016/0014-4827(81)90190-7

    Article  Google Scholar 

  • PJ Ringens HJ Hoenders H Bloemendal (1982) ArticleTitleProtein distribution and characterization in the prenatal and postnatal human lens Exp Eye Res 34 815–823 Occurrence Handle7084342 Occurrence Handle1:CAS:528:DyaL38XktlKlu7c%3D Occurrence Handle10.1016/0014-4835(82)90054-9

    Article  PubMed  CAS  Google Scholar 

  • KR Robinson JW Patterson (1982–83) ArticleTitleLocalization of steady currents in the lens Curr Eye Res 2 843–847

    Google Scholar 

  • A Sandilands AR Prescott JM Carter AM Hutcheson RA Quinlan J Richards PG FitzGerald (1995) ArticleTitleVimentin and CP49/filensin form distinct networks in the lens which are independently modulated during lens fiber cell differentiation J Cell Sci 108 1397–1406 Occurrence Handle7615661 Occurrence Handle1:CAS:528:DyaK2MXlsVSrurY%3D

    PubMed  CAS  Google Scholar 

  • H Schaefer K Marcus A Sickmann M Herrmann J Klose HE Meyer (2003) ArticleTitleIdentification of phosphorylation and acetylation sites in alpha A-crystallin of the eye lens (mus musculus) after two-dimensional gel electrophoresis Anal Bioanal Chem 376 966–972 Occurrence Handle12856102 Occurrence Handle1:CAS:528:DC%2BD3sXmtVGrs74%3D Occurrence Handle10.1007/s00216-003-1983-1

    Article  PubMed  CAS  Google Scholar 

  • C Scheler EC Müller J Stahl U Müller-Werdan J Salnikow P Jungblut (1997) ArticleTitleIdentification and characterization of heat shock protein 27 protein species in human myocardial 2-DE patterns Electrophoresis 18 2823–2831 Occurrence Handle9504816 Occurrence Handle1:CAS:528:DyaK1cXhvFaksLg%3D Occurrence Handle10.1002/elps.1150181518

    Article  PubMed  CAS  Google Scholar 

  • KL Schey M Little JG Fowler RK Crouch (2000) ArticleTitleCharacterization of human lens major intrinsic protein structure Invest Ophthalmol Vis Sci 41 175–182 Occurrence Handle10634618 Occurrence Handle1:STN:280:DC%2BD3c%2FpvVWjtA%3D%3D

    PubMed  CAS  Google Scholar 

  • JG Schoenmakers JJ Gerding H Bloemendal (1969) ArticleTitleThe subunit structure of alpha-crystallin. Isolation and characterization of the S-carboxymethylated acidic subunits from adult and embryonic origin Eur J Biochem 11 472–481 Occurrence Handle4983849 Occurrence Handle1:CAS:528:DyaE3cXltV2qtQ%3D%3D Occurrence Handle10.1111/j.1432-1033.1969.tb00797.x

    Article  PubMed  CAS  Google Scholar 

  • M Shih KJ Lampi TR Shearer LL David (1998) ArticleTitleCleavage of beta crystallins during maturation of bovine lens Mol Vis 4 4–11 Occurrence Handle9485487 Occurrence Handle1:STN:280:DyaK1c7ltlamsg%3D%3D

    PubMed  CAS  Google Scholar 

  • T Shinohara EA Robinson E Appella J Piatigorsky (1982) ArticleTitleMultiple gamma-crystallins of the mouse lens: fractionation of mRNAs by cDNA cloning Proc Natl Acad Sci USA 79 2783–2787 Occurrence Handle6953430 Occurrence Handle1:CAS:528:DyaL38XitFWrsrk%3D Occurrence Handle10.1073/pnas.79.9.2783

    Article  PubMed  CAS  Google Scholar 

  • C Slingsby (1985) ArticleTitleStructural variation in lens crystallins Trends Biochem Sci 10 281–284 Occurrence Handle1:CAS:528:DyaL2MXltVSntLc%3D Occurrence Handle10.1016/0968-0004(85)90088-X

    Article  CAS  Google Scholar 

  • C Slingsby OA Bateman (1990) ArticleTitleQuaternary interactions in eye lens beta-crystallins: basic and acidic subunits of beta-crystallins favor heterologous association Biochemistry 29 6592–6599 Occurrence Handle2397202 Occurrence Handle1:CAS:528:DyaK3cXktlKltrs%3D Occurrence Handle10.1021/bi00480a007

    Article  PubMed  CAS  Google Scholar 

  • C Slingsby B Norledge A Simpson OA Bateman G Wright HPC Driessen PF Lindley DS Moss B Bax (1996) ArticleTitleX-ray diffraction and structure of crystallins Prog Retin Eye Res 16 3–29 Occurrence Handle10.1016/S1350-9462(96)00018-3

    Article  Google Scholar 

  • JB Smith Y Liu DL Smith (1996) ArticleTitleIdentification of possible regions of chaperone activity in lens alpha-crystallin Exp Eye Res 63 125–128 Occurrence Handle8983959 Occurrence Handle1:CAS:528:DyaK28XkvVWjtL4%3D Occurrence Handle10.1006/exer.1996.0100

    Article  PubMed  CAS  Google Scholar 

  • JB Smith Y Sun DL Smith B Green (1992) ArticleTitleIdentification of the posttranslational modifications of bovine lens alpha B-crystallins by mass spectrometry Protein Sci 1 601–608 Occurrence Handle1304359 Occurrence Handle1:CAS:528:DyaK3sXisV2qtro%3D Occurrence Handle10.1002/pro.5560010506

    Article  PubMed  CAS  Google Scholar 

  • JB Smith G Thevenon-Emeric DL Smith B Green (1991) ArticleTitleElucidation of the primary structures of proteins by mass spectrometry Anal Biochem 193 118–124 Occurrence Handle2042736 Occurrence Handle1:CAS:528:DyaK3MXhs1Sitb0%3D Occurrence Handle10.1016/0003-2697(91)90050-4

    Article  PubMed  CAS  Google Scholar 

  • A Spector R Chiesa J Sredy W Garner (1985) ArticleTitlecAMP-dependent phosphorylation of bovine lens alpha-crystallin Proc Natl Acad Sci USA 82 4712–4716 Occurrence Handle2991889 Occurrence Handle1:CAS:528:DyaL2MXkvVelsbs%3D Occurrence Handle10.1073/pnas.82.14.4712

    Article  PubMed  CAS  Google Scholar 

  • AN Srinivasan CN Nagineni SP Bhat (1992) ArticleTitlealpha A-crystallin is expressed in non-occular tissues J Biol Chem 267 23337–23341 Occurrence Handle1429679 Occurrence Handle1:CAS:528:DyaK38XlvVyktLs%3D

    PubMed  CAS  Google Scholar 

  • OP Srivastava MC Kirk K Srivastava (2004) ArticleTitleCharacterization of covalent multimers of crystallins in aging human lenses J Biol Chem 279 10901–10909 Occurrence Handle14623886 Occurrence Handle1:CAS:528:DC%2BD2cXitFyitbw%3D Occurrence Handle10.1074/jbc.M308884200

    Article  PubMed  CAS  Google Scholar 

  • OP Srivastava K Srivastava (1998) ArticleTitleDegradation of gamma D- and gamma S-crystallins in human lenses Biochem Biophys Res Commun 253 288–294 Occurrence Handle9878530 Occurrence Handle1:CAS:528:DyaK1MXhs12htQ%3D%3D Occurrence Handle10.1006/bbrc.1998.9728

    Article  PubMed  CAS  Google Scholar 

  • OP Srivastava K Srivastava V Harrington (1999) ArticleTitleAge-related degradation of beta A3/A1-crystallin in human lenses Biochem Biophys Res Commun 258 632–638 Occurrence Handle10329436 Occurrence Handle1:CAS:528:DyaK1MXjtVSjsr0%3D Occurrence Handle10.1006/bbrc.1999.0506

    Article  PubMed  CAS  Google Scholar 

  • P Stiuso T Libondi AM Facchiano P Colicchio P Ferranti S Lilla G Colonna (2002) ArticleTitleAlteration in the ubiquitin structure and function in the human lens: a possible mechanism of senile cataractogenesis FEBS Lett 531 162–167 Occurrence Handle12417305 Occurrence Handle1:CAS:528:DC%2BD38Xotl2ntbs%3D Occurrence Handle10.1016/S0014-5793(02)03494-4

    Article  PubMed  CAS  Google Scholar 

  • SK Swanson MP Washburn (2005) ArticleTitleThe continuing evolution of shotgun proteomics Drug Discov Today 10 719–725 Occurrence Handle15896685 Occurrence Handle1:CAS:528:DC%2BD2MXkslalt70%3D Occurrence Handle10.1016/S1359-6446(05)03450-1

    Article  PubMed  CAS  Google Scholar 

  • LJ Takemoto (1995) ArticleTitleIdentification of the in vivo truncation sites at the C-terminal region of alpha-A crystallin from aged bovine and human lens Curr Eye Res 14 837–841 Occurrence Handle8529423 Occurrence Handle1:STN:280:DyaK287gtVSjtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • LJ Takemoto (1996) ArticleTitleDifferential phosphorylation of alpha-A crystallin in human lens of different age Exp Eye Res 62 499–504 Occurrence Handle8759518 Occurrence Handle1:CAS:528:DyaK28XjtlOltLk%3D Occurrence Handle10.1006/exer.1996.0060

    Article  PubMed  CAS  Google Scholar 

  • LJ Takemoto (1997) ArticleTitleDisulfide bond formation of cysteine-37 and cysteine-66 of beta B2 crystallin during cataractogenesis of the human lens Exp Eye Res 64 609–614 Occurrence Handle9227279 Occurrence Handle1:CAS:528:DyaK2sXjtFKrsrw%3D Occurrence Handle10.1006/exer.1996.0247

    Article  PubMed  CAS  Google Scholar 

  • K Tanaka H Waki Y Ido S Akita Y Yoshida T Yoshida (1988) ArticleTitleProtein and polymer analysis up to m/z 100,000 by laser ionization time-of-flight mass spectrometry Rapid Commun Mass Spectrom 2 151–153 Occurrence Handle1:CAS:528:DyaK3cXjtVSgug%3D%3D Occurrence Handle10.1002/rcm.1290020802

    Article  CAS  Google Scholar 

  • A Tardieu D Laporte P Licinio B Krop M Delaye (1986) ArticleTitleCalf lens alpha-crystallin quaternary structure. A three-layer tetrahedral model J Mol Biol 192 711–724 Occurrence Handle3586011 Occurrence Handle1:CAS:528:DyaL2sXhvFWgtLo%3D Occurrence Handle10.1016/0022-2836(86)90023-9

    Article  PubMed  CAS  Google Scholar 

  • P Thampi EC Abraham (2003) ArticleTitleInfluence of the C-terminal residues on oligomerization of alpha A-crystallin Biochemistry 42 11857–11863 Occurrence Handle14529298 Occurrence Handle1:CAS:528:DC%2BD3sXnt1Olur0%3D Occurrence Handle10.1021/bi030129w

    Article  PubMed  CAS  Google Scholar 

  • P Thampi A Hassan JB Smith EC Abraham (2002) ArticleTitleEnhanced C-terminal truncation of alpha A- and alpha B-crystallins in diabetic lenses Invest Ophthalmol Vis Sci 43 3265–3272 Occurrence Handle12356833

    PubMed  Google Scholar 

  • H Toyofuku PJ Bentley (1970) ArticleTitleThe effects of decapsulation on ion movements across the lens of the toad, Bufo marinus. Influence on drug actions Invest Ophthalmol 9 959–965 Occurrence Handle5530208 Occurrence Handle1:CAS:528:DyaE3MXntl2isg%3D%3D

    PubMed  CAS  Google Scholar 

  • SJ Tumminia JI Clark DM Richiert KP Mitton Y Duglas-Tabor JA Kowalak DL Garland P Russell (2001) ArticleTitleThree distinct stages of lens opacification in transgenic mice expressing the HIV-1 protease Exp Eye Res 72 115–121 Occurrence Handle11161727 Occurrence Handle1:CAS:528:DC%2BD3MXmtV2ntw%3D%3D Occurrence Handle10.1006/exer.2000.0934

    Article  PubMed  CAS  Google Scholar 

  • Y Ueda MK Duncan LL David (2002a) ArticleTitleLens proteomics: the accumulation of crystallin modifications in the mouse lens with age Invest Ophthalmol Vis Sci 43 205–215

    Google Scholar 

  • Y Ueda C Fukiage M Shih TR Shearer LL David (2002b) ArticleTitleMass measurements of C-terminally truncated alpha-crystallins from two-dimensional gels identify Lp82 as a major endopeptidase in rat lens Mol Cell Proteomics 1 357–365 Occurrence Handle1:CAS:528:DC%2BD38XlslWhsrs%3D Occurrence Handle10.1074/mcp.M200007-MCP200

    Article  CAS  Google Scholar 

  • Y Ueda AL McCormack TR Shearer LL David (2001) ArticleTitlePurification and characterization of lens specific calpain (Lp82) from bovine lens Exp Eye Res 73 625–637 Occurrence Handle11747363 Occurrence Handle1:CAS:528:DC%2BD3MXptFWrtLc%3D Occurrence Handle10.1006/exer.2001.1071

    Article  PubMed  CAS  Google Scholar 

  • PJ van den Oetelaar PF van Someren JA Thomson RJ Siezen HJ Hoenders (1990) ArticleTitleA dynamic quaternary structure of bovine alpha-crystallin as indicated from intermolecular exchange of subunits Biochemistry 29 3488–3493 Occurrence Handle2354148 Occurrence Handle1:CAS:528:DyaK3cXhsFeru74%3D Occurrence Handle10.1021/bi00466a010

    Article  PubMed  CAS  Google Scholar 

  • FJ van der Ouderaa WW de Jong H Bloemendal (1973) ArticleTitleThe amino-acid sequence of the alpha A2 chain of bovine alpha-crystallin Eur J Biochem 39 207–222 Occurrence Handle4770792 Occurrence Handle1:CAS:528:DyaE2cXjsVGiuw%3D%3D Occurrence Handle10.1111/j.1432-1033.1973.tb03119.x

    Article  PubMed  CAS  Google Scholar 

  • GL van Rens JM Raats HP Driessen M Oldenburg JT Wijnen PM Khan WW de Jong H Bloemendal (1989) ArticleTitleStructure of the bovine eye lens gamma S-crystallin gene (formerly beta S) Gene 78 225–233 Occurrence Handle2476364 Occurrence Handle1:CAS:528:DyaK3cXhslehtLk%3D Occurrence Handle10.1016/0378-1119(89)90225-4

    Article  PubMed  CAS  Google Scholar 

  • CE Voorter WA de Haard-Hoekman ES Roersma HE Meyer H Bloemendal WW de Jong (1989) ArticleTitleThe in vivo phosphorylation sites of bovine alpha B-crystallin FEBS Lett 259 50–52 Occurrence Handle2599111 Occurrence Handle1:CAS:528:DyaK3cXnt1Gmuw%3D%3D Occurrence Handle10.1016/0014-5793(89)81491-7

    Article  PubMed  CAS  Google Scholar 

  • ST Wang-Su AL McCormack S Yang MR Hosler A Mixon MA Riviere PA Wilmarth UP Andley D Garland H Li LL David BJ Wagner (2003) ArticleTitleProteome analysis of lens epithelia, fibers, and the HLE B-3 cell line Invest Ophthalmol Vis Sci 44 4829–4836 Occurrence Handle14578405 Occurrence Handle10.1167/iovs.03-0556

    Article  PubMed  Google Scholar 

  • MP Washburn D Wolters JR Yates Suffix3rd (2001) ArticleTitleLarge-scale analysis of the yeast proteome by multidimensional protein identification technology Nat Biotechnol 19 242–247 Occurrence Handle11231557 Occurrence Handle1:CAS:528:DC%2BD3MXhslaqtbw%3D Occurrence Handle10.1038/85686

    Article  PubMed  CAS  Google Scholar 

  • VC Wasinger SJ Cordwell A Cerpa-Poljak JX Yan AA Gooley MR Wilkins MW Duncan R Harris KL Williams I Humphrey-Smith (1995) ArticleTitleProgress with gene-product mapping of the Mollicutes: mycoplasma genitalium Electrophoresis 16 1090–1094 Occurrence Handle7498152 Occurrence Handle1:CAS:528:DyaK2MXntF2lsLg%3D Occurrence Handle10.1002/elps.11501601185

    Article  PubMed  CAS  Google Scholar 

  • PJ Werten E Vos WW De Jong (1999) ArticleTitleTruncation of beta A3/A1-crystallin during aging of the bovine lens; possible implications for lens optical quality Exp Eye Res 68 99–103 Occurrence Handle9986747 Occurrence Handle1:CAS:528:DyaK1MXhtVSis7g%3D Occurrence Handle10.1006/exer.1998.0584

    Article  PubMed  CAS  Google Scholar 

  • P Wilmarth JR Taube MA Riviere MK Duncan LL David (2004) ArticleTitleProteomic and sequence analysis of chicken lens crystallins reveals alternate splicing and translational forms of beta B2 and beta A2 crystallins Invest Ophthalmol Vis Sci 45 2705–2715 Occurrence Handle15277495 Occurrence Handle10.1167/iovs.04-0131

    Article  PubMed  Google Scholar 

  • G Wistow C Slingsby T Blundell H Driessen W DeJong H Bloemendal (1981) ArticleTitleEye-lens proteins: the three-dimensional structure of beta-crystallin predicted from monomeric gamma-crystallin FEBS Lett 133 9–16 Occurrence Handle6927387 Occurrence Handle1:CAS:528:DyaL3MXlvF2nsrc%3D Occurrence Handle10.1016/0014-5793(81)80460-7

    Article  PubMed  CAS  Google Scholar 

  • GJ Wistow J Piatigorsky (1988) ArticleTitleLens crystallins: the evolution and expression of proteins for a highly specialized tissue Annu Rev Biochem 57 479–504 Occurrence Handle3052280 Occurrence Handle1:CAS:528:DyaL1cXkvVOjt78%3D Occurrence Handle10.1146/annurev.bi.57.070188.002403

    Article  PubMed  CAS  Google Scholar 

  • G Wistow K Wyatt L David C Gao O Bateman S Bernstein S Tomarev L Segovia C Slingsby T Vihtelic (2005) ArticleTitlegamma N-crystallin and the evolution of the betagamma-crystallin superfamily in vertebrates FEBS J 272 2276–2291 Occurrence Handle15853812 Occurrence Handle1:CAS:528:DC%2BD2MXkt1Wmt7k%3D Occurrence Handle10.1111/j.1742-4658.2005.04655.x

    Article  PubMed  CAS  Google Scholar 

  • DA Wolters MP Washburn JR Yates Suffix3rd (2001) ArticleTitleAn automated multidimensional protein identification technology for shotgun proteomics Anal Chem 73 5683–5690 Occurrence Handle11774908 Occurrence Handle1:CAS:528:DC%2BD3MXnslaqsrw%3D Occurrence Handle10.1021/ac010617e

    Article  PubMed  CAS  Google Scholar 

  • M Yamashita JB Fenn (1984) ArticleTitleElectrospray ion source. Another variation on the free jet theme J Phys Chem 88 4451–4459 Occurrence Handle1:CAS:528:DyaL2cXlsVahs7g%3D Occurrence Handle10.1021/j150664a002

    Article  CAS  Google Scholar 

  • JR Yates Suffix3rd (1998) ArticleTitleMass spectrometry and the age of the proteome J Mass Spectrom 33 1–19 Occurrence Handle9449829 Occurrence Handle1:CAS:528:DyaK1cXnsFSgtA%3D%3D Occurrence Handle10.1002/(SICI)1096-9888(199801)33:1<1::AID-JMS624>3.0.CO;2-9

    Article  PubMed  CAS  Google Scholar 

  • NT Yu DC DeNagel PL Pruett JFR Kuck SuffixJr (1985) ArticleTitleDisulphide bond formation in the eye lens Proc Natl Acad Sci USA 82 207–214 Occurrence Handle10.1073/pnas.82.23.7965

    Article  Google Scholar 

  • S Zarina C Slingsby R Jaenicke ZH Zaidi H Driessen N Srinivasan (1994) ArticleTitleThree-dimensional model and quaternary structure of the human eye lens protein gamma S-crystallin based on beta- and gamma-crystallin X-ray coordinates and ultracentrifugation Protein Sci 3 1840–1846 Occurrence Handle7849599 Occurrence Handle1:CAS:528:DyaK2MXit1yjsLg%3D Occurrence Handle10.1002/pro.5560031023

    Article  PubMed  CAS  Google Scholar 

  • Y Zhen N Xu B Richardson R Becklin JR Savage K Blake JM Peltier (2004) ArticleTitleDevelopment of an LC-MALDI method for the analysis of protein complexes J Am Soc Mass Spectrom 15 803–822 Occurrence Handle15144970 Occurrence Handle1:CAS:528:DC%2BD2cXktVOgsbs%3D Occurrence Handle10.1016/j.jasms.2004.02.004

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Max Planck Institute for Infection Biology, Core Facility Protein Analysis, Berlin, Germany

    W. Hoehenwarter & P. R. Jungblut

  2. Institute for Human Genetics, Charité, Berlin, Germany

    J. Klose

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An erratum to this article is available at http://dx.doi.org/10.1007/s00726-009-0270-7.

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Hoehenwarter, W., Klose, J. & Jungblut, P. Eye lens proteomics. Amino Acids 30, 369–389 (2006). https://doi.org/10.1007/s00726-005-0283-9

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