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Antiangiogenic, Antimicrobial, and Cytotoxic Potential of Sponge-Associated Bacteria

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Abstract

The bacteria associated with marine invertebrates are a rich source of bioactive metabolites. In the present study bacteria associated with the sponge Suberites domuncula and its primmorphs (3-dimensional aggregates containing proliferating cells) were isolated and cultured. These bacteria were extracted, and the extracts were assayed for antiangiogenic, hemolytic, antimicrobial, and cytotoxic activities. Our studies revealed that extract obtained from the bacterium (PB2) isolated from sponge primmorphs is a potent angiogenesis inhibitor. In the chick chorio-allantoic membrane (CAM) assay, it showed 50% activity at 5 μg ml−1 and 100% activity at 10 and 20 μg ml−1 concentrations. Extracts obtained from 5 bacterial strains isolated from sponge and its primmorphs showed hemolytic activity. The sponge-associated bacteria belonging to the α subdivision of Proteobacteria and the primmorph-associated bacterium identified as a possible novel Pseudomonas sp. displayed remarkable antimicrobial activity. It is important to note that these bacterial extracts were strongly active against multidrug-resistant clinical strains such as Staphylococcus aureus and Staphylococcus epidermidis, isolated from hospital patients. The bacterial extracts having antimicrobial activity also showed cytotoxicity against HeLa and PC12 cells. In summary, this investigation explores the importance of sponge-associated bacteria as a valuable resource for the discovery of novel bioactive molecules.

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Acknowledgments

We are grateful to Dr. Ute Hentschel for her help in phylogenetic analysis of these bacteria and also for fruitful discussions during every stage of the work. We thank Dr. Manohar A. Indap, Mumbai, for his inspiration to perform antiangiogenesis studies. This work was financially supported by the Bundesministerium für Bildung und Forschung (project: Center of Excellence “BIOTECmarin”), the European Commission (project: SPONGE), and the International Human Frontier Science Program (RG-333/96-M).

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität Mainz, Duesbergweg 6, D-55099, Mainz, Germany

    Archana N. Thakur, Narsinh L. Thakur & Werner E.G. Müller

  2. Department of Zoology, D.G. Ruparel College, 400016, Mahim, Mumbai, India

    Archana N. Thakur, Madhavi M. Indap, Reena A. Pandit & Vrushali V. Datar

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  1. Archana N. Thakur
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  2. Narsinh L. Thakur
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  3. Madhavi M. Indap
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  6. Werner E.G. Müller
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Correspondence to Werner E.G. Müller.

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Thakur, A.N., Thakur, N.L., Indap, M.M. et al. Antiangiogenic, Antimicrobial, and Cytotoxic Potential of Sponge-Associated Bacteria. Mar Biotechnol 7, 245–252 (2005). https://doi.org/10.1007/s10126-004-4085-y

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