Abstract
The storage of potato tubers at low temperatures leads to the accumulation of sugars in a process called “low-temperature sweetening.” To understand this phenomenon, we measured the sugar contents and the activity of acid invertase over several months in tubers of six Japanese cultivars stored at 4°C or 20°C. At 20°C , few changes in sugar contents took place in any of the tubers. On the other hand, when stored at 4°C, three types of changes were observed among the cultivars: (1) increased levels of reducing sugars during storage; (2) a pattern similar to that of type 1, but with 4- to 6-fold lower levels of reducing sugars throughout storage; and (3) increased sucrose, but not reducing sugars. The activity of vacuolar acid invertase increased in the type-1 cultivars, whereas, in the type-2 and type-3 cultivars, the activities were very low during storage at 4°C. Reverse transcription-polymerase chain reaction analysis of acid invertase showed that the transcripts of the enzyme accumulated in the tubers stored at 4°C in the type-1 cultivars but not in type-3. These results suggest that the activity of vacuolar acid invertase is related to the types of changes that occurred in sugar content during low-temperature storage among the potato cultivars.
Similar content being viewed by others
References
Blenkinsop RW, Copp LJ, Yada RY, Marangoni AG (2002) Changes in compositional parameters of tubers of potato (Solanum tuberosum) during low-temperature storage and their relationship to chip processing quality. J Agric Food Chem 50:4545–4553
Bracho GE, Whitaker JR (1990) Characteristics of the inhibition of potato (Solanum tuberosum) invertase by an endogenous proteinaceous inhibitor in potatoes. Plant Physiol 92:381–385
Burton WG (1989) The potato, 3rd edn. Longman Scientific and Technical, New York
Chuda Y, Ono H, Yada H, Ohara-Takada A, Matsuura-Endo C, Mori M (2003) Effect of physiological change in potato tuber (Solanum tuberosum L.) after low temperature storage on the level of acrylamide formed in potato chips. Biosci Biotechnol Biochem 67:1188–1190
Davies HV, Oparka KJ (1985) Hexose metabolism in developing tubers of potato (Solanum tuberosum L.) cv Maris Piper. J Plant Physiol 119:311–316
Davies HV, Viola R (1994) Control of sugar balance in potato tubers. In: Belknap WR, Vayda ME, Park WD (eds) The molecular and cellular biology of the potato, 2nd edn. CAB International, Wallingford, UK, pp 67–80
Espen L, Morgutti S, Abruzzese A, Negrini N, Rivetta A, Quattrini MM, Cocucci M, Cocucci SM (1999) Changes in the potato (Solanum tuberosum L.) tuber at the onset of dormancy and during storage at 23°C and 3°C. I. Biochemical and physiological parameters. Potato Res 42:189–201
Ewing EE, McAdoo MH (1971) An examination of methods used to assay potato tuber invertase and its naturally occurring inhibitor. Plant Physiol 48:366–370
Hammond JBW, Burrell MM, Kruger NJ (1990) Effect of low temperature on the activity of phosphofructokinase from potato tubers. Planta 180:613–616
Hill LM, Reimholz R, Schröder R, Nielsen TH, Stitt M (1996) The onset of sucrose accumulation in cold-stored potato tubers is caused by an increased rate of sucrose synthesis and coincides with low levels of hexose-phosphates, an activation of sucrose phosphate synthase and the appearance of a new form of amylase. Plant Cell Environ 19:1223–1237
Isherwood FA (1973) Starch-sugar interconversion in Solanum tuberosum. Phytochemistry 12:2579–2591
Ishii G, Mori M, Ohara A, Umemura Y (1999) Food chemical properties of a new potato with orange flesh. In: Hagg M, Ahvenainen R, Evers AM, Tiilikkala K (eds) Agri-food quality. II. Quality management of fruits and vegetables. RSC, Wallingford, UK, pp 357–359
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Pollock C, ap Rees T (1975) Activities of enzymes of sugar metabolism in cold-stored tubers of Solanum tuberosum. Phytochemistry 14:613–617
Pressey R (1967) Invertase inhibitor from potatoes: purification, characterization, and reactivity with plant invertases. Plant Physiol 42:1780–1786
Pressey R (1969a) Role of invertase in the accumulation of sugars in cold-stored potatoes. Am Potato J 46:291–297
Pressey R (1969b) Potato sucrose synthetase: purification, properties, and changes in activity associated with maturation. Plant Physiol 44:759–764
Richardson DL, Davies HV, Ross HA, Mackay GR (1990) Invertase activity and its relation to hexose accumulation in potato tubers. J Exp Bot 41:95–99
Ross HA, Davies HV (1992) Sucrose metabolism in tubers of potato (Solanum tuberosum L.): effect of sink removal and sucrose flux on sucrose-degrading enzymes. Plant Physiol 98:287–293
Shirzadegan M, Christie P, Seemann JR (1991) An efficient method for isolation of RNA from tissue cultured plant cells. Nucleic Acids Res 19:6055
Sowokinos JS (1990) Stress-induced alterations in carbohydrate metabolism. In: Vayda ME, Park WD (eds) The molecular and cellular biology of the potato. CAB International, Wallingford, UK, pp 137–158
Sowokinos J (1994) Post-harvest regulation of sucrose accumulation in transgenic potatoes: role and properties of potato tuber UDP-glucose pyrophosphorylase. In: Belknap WR, Vayda ME, Park WD (eds) The molecular and cellular biology of the potato, 2nd edn. CAB International, Wallingford, UK, pp 81–106
Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M (2002) Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem 50:4998–5006
Zhou D, Mattoo A, Li N, Imaseki H, Solomos T (1994) Complete nucleotide sequence of potato tuber acid invertase cDNA. Plant Physiol 106:397–398
Zrenner R, Schuler K, Sonnewald U (1996) Soluble acid invertase determines the hexose-to-sucrose ratio in cold-stored potato tubers. Planta 198:246–252
Acknowledgement
This work was partially supported by a Grant-in-Aid for the Research and Development Program for New Bio-industry Initiatives from the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Matsuura-Endo, C., Kobayashi, A., Noda, T. et al. Changes in sugar content and activity of vacuolar acid invertase during low-temperature storage of potato tubers from six Japanese cultivars. J Plant Res 117, 131–137 (2004). https://doi.org/10.1007/s10265-003-0137-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10265-003-0137-z