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Two phases of intracellular reactive oxygen species production during victorin-induced cell death in oats

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Abstract

Reactive oxygen species (ROS) are thought to be involved in various forms of programmed cell death (PCD) in animal and plant cells. PCD, along with the production of ROS, occurs during plant–pathogen interactions. Here we show that victorin, a host-specific toxin produced by Cochliobolus victoriae, which causes victoria blight of oats, induces two phases of intracellular ROS production in victorin-sensitive oat mesophyll cells. The initial production of ROS is restricted at mitochondria and not accompanied with cellular oxidative damage. Later production of ROS is dispersed into cells concomitant with lipid peroxidation, chloroplast dysfunction, and cell death. Superoxide dismutase can clearly suppress the initial ROS production and delay the progression of cell death. These data indicate that the initial ROS production may be involved in the cell death induction process, and the later ROS production may play important roles in events leading to cellular disruption.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Graduate School of Science and Technology, Faculty of Agriculture, Kobe University, Kobe, 657-8501, Japan

    Masaru Sakamoto, Yasuomi Tada, Hitoshi Nakayashiki, Yukio Tosa & Shigeyuki Mayama

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  1. Masaru Sakamoto
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  2. Yasuomi Tada
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  3. Hitoshi Nakayashiki
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  4. Yukio Tosa
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  5. Shigeyuki Mayama
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Correspondence to Shigeyuki Mayama.

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Sakamoto, M., Tada, Y., Nakayashiki, H. et al. Two phases of intracellular reactive oxygen species production during victorin-induced cell death in oats. J Gen Plant Pathol 71, 387–394 (2005). https://doi.org/10.1007/s10327-005-0220-5

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  • DOI: https://doi.org/10.1007/s10327-005-0220-5

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