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Resistance to the two-spotted spider mite (Tetranychus urticae) by acylsucroses of wild tomato (Solanum pimpinellifolium) trichomes studied in a recombinant inbred line population

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Abstract

Trichome-based host plant resistance is a complex mechanism that could be used in tomato breeding to control arthropod pests. The aims of this work were to evaluate the plant traits (density of trichomes and acylsucrose production) and the functional relationships of these traits with mortality, repellence, and oviposition of Tetranychus urticae Koch (Acari: Tetranychidae). We used a population of recombinant inbred lines (RILs) derived from the cross between the wild tomato, Solanum pimpinellifolium L. ‘TO-937’, and the cultivated tomato, Solanum lycopersicum L. Multiple regression analyses showed that high acylsucrose content and high type-IV trichome density increased mortality and repellence, and reduced oviposition of T. urticae. Single regression analyses showed that a logistic model best explained the relationship between mortality or repellence and acylsucrose content, whereas a negative-exponential model best described the relationship between oviposition and acylsucrose content. Linear models were the best-fits for the three resistance variables with trichome IV density. Probit analysis was used to estimate acylsucrose effective doses, and revealed that 31 and 10% of the RILs produced acylsucrose above the effective doses for 90% mortality or repellence, respectively. Altogether, these results indicate that S. pimpinellifolium may be a suitable genetic source of resistance to spider mites to be used in cultivated tomato.

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Acknowledgments

This study is part of the research projects AGL2004-06985-C02-01 and AGL2007-66760-C02-02, co-funded by the Ministerio de Educación y Ciencia (MEC, Spain) and the Fondo Europeo de Desarrollo Regional (FEDER, EU). J.M.A. was a recipient of a pre-doctoral I3P fellowship from CSIC-Fondo Social Europeo. M.M. was employed by the CSIC within the framework of a post-doctoral JAE position. We thank three anonymous reviewers for their useful comments.

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Authors and Affiliations

  1. Estación Experimental La Mayora – CSIC, Algarrobo-Costa, 29760, Málaga, Spain

    Juan Manuel Alba, Marta Montserrat & Rafael Fernández-Muñoz

  2. Department of Population Biology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 94084, 1090 GB, Amsterdam, The Netherlands

    Juan Manuel Alba

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  1. Juan Manuel Alba
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  2. Marta Montserrat
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Correspondence to Juan Manuel Alba.

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Alba, J.M., Montserrat, M. & Fernández-Muñoz, R. Resistance to the two-spotted spider mite (Tetranychus urticae) by acylsucroses of wild tomato (Solanum pimpinellifolium) trichomes studied in a recombinant inbred line population. Exp Appl Acarol 47, 35–47 (2009). https://doi.org/10.1007/s10493-008-9192-4

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