Abstract
Termites, Coptotermes formosanus, reared individually, were highly susceptible to the entomopathogenic fungus, Metarhizium anisopliae, while termites reared in␣groups were highly resistant. When reared in groups, the termites treated with M.␣anisopliae conidia on the body surface were groomed by their nestmates and more than 80% of the conidia were removed from the cuticle within 3 h. However, there was not a significant reduction in the numbers of conidia on the body surfaces of termites reared individually. For the termites maintained in groups, conidia were found in foreguts, midguts and hindguts, but very few conidia were detected in the guts of termites reared individually. Conidia in the alimentary tracts did not germinate, but some of were alive. As a result, it seems that the removal of foreign bodies, such as fungal conidia, from the␣cuticle is one function of termite mutual grooming behavior and that conidia removed from the cuticle are eliminated through alimentary tracts. This study indicates that mutual grooming behavior is very effective in protecting these termites from M.␣anisopliae infection.
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References
Boucias D.G., Stokes C., Storey G., and Pendland J.C. (1996). The effects of imidacloprid on the termites Reticulitermes flavipes and its interaction with the mycopathogen Beauveria bassiana. Pflanzenschutz-Nachr. Bayer 49:103–144
Dillon R.J., and Chanley A.K. (1988). Inhibition of Metarhizium anisopliae by the gut bacterial flora of the desert locust: characterization of antifungal toxin. Can. J. Microbiol. 34:1075–1082
Hughes W.O.H., Eilenberg J., and Boomsma J.J. (2002). Trade-offs in group living: transmission and disease resistance in leaf-cutting ants. Proc. R. Soc. Lond., Ser. B: Biol. Sci. 269:1811–1819
Hung S.T., and Boucias D.G. (1992). Influence of Beauveria bassiana on the cellular defense response of the beet armyworm, Spodoptera exigua. J. Invertebr. Pathol. 60:152–158
Kramm K.R., and West D.F. (1982). Termite pathogens: effects of ingested Metarhizium, Beauveria, and Gliocladium conidia on worker termite (Reticulitermes sp.). J. Invertebr. Pathol. 40:7–11
Rosengaus R.B., and Traniello J.F.A. (2001). Disease susceptibility and the adaptive nature of colony demography in the dampwood termite Zootermophsis angusticollis. Behav. Ecol. Sociobiol. 50:546–556
SAS Institute Inc (1999). SAS 9.1.3 Service Pack 1. SAS Institute Inc., Cary, NC
Shimizu S., and Yamaji M. (2003). Effect of density of the termite, Reticulitermes speratus Kolbe (Isoptera: Rhinotermitidae), on the susceptibilities to Metarhizium anisopliae. Jap. J. Appl. Entomol. Zool. 38:125–135
Vargo E.L., Husseneder C., and Grace J.K. (2003) Colony and population genetic structure of the Formosan subterranean termite, Coptotermes formosanus, in Japan. Molecu. Ecol. 12(10):2599–2608
Yaginuma K. (1990). Detection of fungi to peach fruit moth, Carposina niponensis Walsingham from soil. Bull. Fruit Tree Res. Stn. 17:77–89 (in Japanese with English summary)
Yanagawa A., and Shimizu S. (2005). Defense strategy of the termite, Coptotermes foumosanus Shiraki to entomopathogenic fungi. Jpn. J. Environ. Entomol. Zool. 16:17–22
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We thank the editor of BioControl and two anonymous reviewers for guidance and comments on the manuscript, especially about statistical analysis.
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Yanagawa, A., Shimizu, S. Resistance of the termite, Coptotermes formosanus Shiraki to Metarhizium anisopliae due to grooming. Biocontrol 52, 75–85 (2007). https://doi.org/10.1007/s10526-006-9020-x
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DOI: https://doi.org/10.1007/s10526-006-9020-x