Abstract
Plant growth-promoting rhizobacteria with gibberellins (GA)-producing potential were isolated from soil and screened for plant growth promotion. A new strain, Acinetobacter calcoaceticus SE370, produced extracellular GA and also had phosphate solubilising potential. It produced 10 different gibberellins, including the bioactive GA1, GA3 and GA4 which were at, respectively, 0.45, 6.2 and 2.8 ng/100 ml. The isolate solubilised tricalcium phosphate and lowered pH of the medium during the process. Culture filtrates of the organism after growth on broth promoted growth of cucumber, Chinese cabbage and crown daisy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adachi M, Sako Y, Ishida Y (1996) Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions. J Phycol 32:424–432
Amies CR (1967) A modified formula for the preparation of Stuart’s transport medium. Can J Public Health 58:296–300
Cassán F, Lucangeli C, Bottini R et al (2001) Azospirillum spp. metabolize [17,17-2H2]gibberellin A20 to [17,17-2H2]gibberellin A1 in vivo in dry rice mutant seedlings. Plant Cell Physiol 42:763–767
Dobert RC, Rood SB, Blevins DG (1992) Gibberellins and the legume-Rhizobium symbiosis. I. Endogenous gibberellins of lima bean (Phaseolus lunatus L.) stems and nodules. Plant Physiol 98:221–224
Gaskin P, MacMillan J (1991) GC-MS of gibberellins and related compounds. Methodology and a library of reference spectra. Cantocks Enterprises, Bristol, UK
Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117
Joo G-J, Kim Y-M, Lee I-J et al (2004) Growth promotion of red pepper plug seedlings and the production of gibberellins by Bacillus cereus, Bacillus macroides and Bacillus pumilus. Biotechnol Lett 26:487–491
Kpomblekou AK, Tabatabai MA (1994) Effect of organic acids on release of P from phosphate rocks. Soil Sci 158:442–443
Lee I-J, Foster KR, Morgan PW (1998) Photoperiod control of gibberellin levels and flowering in sorghum. Plant Physiol 116:1003–1010
Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Ecol 170:265–270
Nautiyal CS, Bhaduria S, Kumar P et al (2000) Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiol Lett 182:291–296
Peix A, Rivas-Boyero AA, Mateos PF et al (2002) Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol Biochem 33:103–110
Piccoli P, Masciarelli O, Bottini R (1999) Gibberellin production by Azospirillum lipoferum cultured in chemically-defined medium as affected by oxygen availability and water status. Symbiosis 27:135–146
Rodríguez H, Fraga R (1999) Phosphate-solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Acknowledgments
This research project was supported by the Korea Research Foundation Grant (KRF-2004-005-F00062).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Kang, SM., Joo, GJ., Hamayun, M. et al. Gibberellin production and phosphate solubilization by newly isolated strain of Acinetobacter calcoaceticus and its effect on plant growth. Biotechnol Lett 31, 277–281 (2009). https://doi.org/10.1007/s10529-008-9867-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-008-9867-2