Skip to main content
SpringerLink
Log in

Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA)

  • Method in Cell Science
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Cell viability and cell migration capacities are critical parameters for cell culture-related studies. It is essential to monitor the dynamic changes of cell properties under various co-culture conditions to our better understanding of their behaviours and characteristics. The real time cell analyzer (RTCA, xCELLigence, Roche) is an impedance-based technology that can be used for label-free and real-time monitoring of cell properties, such as cell adherence, proliferation, migration and cytotoxicity. The practicality of this system has been proven in our recent cancer studies. In the present method, we intend to use co-cultures of pancreatic cancer cells (HP62) and mesenchymal stem cells to describe in detail, the procedures and benefits of RTCA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med 115:453–466

    Article  CAS  Google Scholar 

  • Dai LJ, Moniri MR, Zeng ZR, Zhou JX, Rayat J, Warnock GL (2011) Potential implications of mesenchymal stem cells in cancer therapy. Cancer Lett 305:8–20. doi:10.1016/j.canlet.2011.02.012

    Article  CAS  Google Scholar 

  • Duncan AW, Rattis FM, DiMascio LN, Congdon KL, Pazianos G, Zhao C, Yoon K, Cook JM, Willert K, Gaiano N, Reya T (2005) Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Nat Immunol 6:314–322. doi:10.1038/ni1164

    Article  CAS  Google Scholar 

  • Friedenstein AJ, Piatetzky S II, Petrakova KV (1966) Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 16:381–390

    CAS  Google Scholar 

  • Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP (1968) Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 6:230–247

    Article  CAS  Google Scholar 

  • Krishan A (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66:188–193

    Article  CAS  Google Scholar 

  • Lingen MW (2003) Endothelial cell migration assay. A quantitative assay for prediction of in vivo biology. Methods Mol Med 78:337–347

    Google Scholar 

  • Menon MB, Ronkina N, Schwermann J, Kotlyarov A, Gaestel M (2009) Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration. Cell Motil Cytoskelet 66:1041–1047

    Article  CAS  Google Scholar 

  • Moniri MR, Sun XY, Rayat J, Dai D, Ao Z, He Z, Verchere CB, Dai LJ, Warnock GL (2012) TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells. Cancer Gene Ther 19:652–658. doi:10.1038/cgt.2012.46

    Article  CAS  Google Scholar 

  • Qiao L, Xu Z, Zhao T, Zhao Z, Shi M, Zhao RC, Ye L, Zhang X (2008) Suppression of tumorigenesis by human mesenchymal stem cells in a hepatoma model. Cell Res 18:500–507. doi:10.1038/cr.2008.40

    Article  CAS  Google Scholar 

  • Reya T, Morrison SJ, Clarke MF, Weissman IL (2001) Stem cells, cancer, and cancer stem cells. Nature 414:105–111. doi:10.1038/35102167

    Article  CAS  Google Scholar 

  • Sasportas LS, Kasmieh R, Wakimoto H, Hingtgen S, van de Water JA, Mohapatra G, Figueiredo JL, Martuza RL, Weissleder R, Shah K (2009) Assessment of therapeutic efficacy and fate of engineered human mesenchymal stem cells for cancer therapy. Proc Natl Acad Sci USA 106:4822–4827. doi:10.1073/pnas.0806647106

    Article  CAS  Google Scholar 

  • Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV (1992) Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13:204–208. doi:10.1002/cyto.990130216

    Article  CAS  Google Scholar 

  • Shimokawa I, Higami Y, Horiuchi S, Iwasaki M, Ikeda T (1998) Advanced glycosylation end products in adrenal lipofuscin. J Gerontol Ser A Biol Sci Med Sci 53:B49–B51

    Article  CAS  Google Scholar 

  • Siegel R, Naishadham D, Jemal A (2012) Cancer statistics, 2012. CA Cancer J Clin 62:10–29. doi:10.3322/caac.20138

    Article  Google Scholar 

  • Sun XY, Nong J, Qin K, Lu H, Moniri MR, Dai LJ, Warnock GL (2011) MSC (TRAIL)-mediated HepG2 cell death in direct and indirect co-cultures. Anticancer Res 31:3705–3712

    CAS  Google Scholar 

  • Wang L, Zhu J, Deng C, Xing WL, Cheng J (2008) An automatic and quantitative on-chip cell migration assay using self-assembled monolayers combined with real-time cellular impedance sensing. Lab Chip 8:872–878

    Article  CAS  Google Scholar 

  • Yang B, Wu X, Mao Y, Bao W, Gao L, Zhou P, Xie R, Zhou L, Zhu J (2009) Dual-targeted antitumor effects against brainstem glioma by intravenous delivery of tumor necrosis factor-related, apoptosis-inducing, ligand-engineered human mesenchymal stem cells. Neurosurgery 65:610–624. doi:10.1227/01.NEU.0000350227.61132.A7 discussion 624

    Article  Google Scholar 

Download references

Acknowledgments

The work was supported by the VGH and UBC Hospital Foundation. We thank Dr. Paul Rennie, Dr. Melissa Brierley, and Dr. Eric LeBlanc who have been tremendous resources throughout our work. We are grateful to Mrs. Crystal Robertson and Dr. Miriam Butler for their assistance editing the manuscript.

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Surgery, University of British Columbia, Vancouver, BC, Canada

    Mani Roshan Moniri, Ada Young, Kelsey Reinheimer, Jarrett Rayat, Long-Jun Dai & Garth L. Warnock

Authors
  1. Mani Roshan Moniri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ada Young
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kelsey Reinheimer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jarrett Rayat
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Long-Jun Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Garth L. Warnock
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Mani Roshan Moniri or Long-Jun Dai.

Additional information

Mani Roshan Moniri and Ada Young are the co-authors.

Kelsey Reinheimer and Jarrett Rayat contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roshan Moniri, M., Young, A., Reinheimer, K. et al. Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA). Cytotechnology 67, 379–386 (2015). https://doi.org/10.1007/s10616-014-9692-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-014-9692-5

Keywords

Search

Navigation