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Identification of HIF-1α promoter and expression regulation of HIF-1α gene by LPS and hypoxia in zebrafish

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Abstract

The ubiquitously expressed hypoxia-inducible factor-1α (HIF-1α) acts as a key transcription factor in regulating metabolism, development, cellular survival, proliferation and pathology under hypoxia condition. Compared to mammals, fish are more vulnerable to hypoxia stress and contamination; however, the regulation of HIF-1α in fish remains obscure. In this study, zebrafish HIF-1α promoter was cloned and found to possess a CpG island located at −97 to +403, but the canonical TATA-box was absent. Aligning 240-bp HIF-1α proximal promoter region of zebrafish with other vertebrates showed more than 82 % identity with cyprinid fishes. Further luciferase analysis suggested that the minimal core promoter might locate at −134 to +97, and several putative transcription factor binding sites were found in this region by bioinformatic analysis. Moreover, it was shown that the zebrafish HIF-1α mRNA was significantly activated by 10 μg/mL lipopolysaccharide (LPS) under hypoxia condition and peaked at 8 h after treatment, suggesting LPS- and hypoxia-regulated zebrafish HIF-1α transcriptional activity in a synergistic pattern. This synergistic effect was closely related to the living environment of fish, indicating that this mechanism would be more conducive to fish survival.

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Acknowledgments

The research was supported by the natural science foundation of China (30901099), program for new century excellent talents in university (NCET-10-0403) and the fundamental research funds for the central universities (2011PY072, 2010PY004).

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  1. Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Shasha Liu, Kecheng Zhu, Nan Chen, Weimin Wang & Huanling Wang

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  1. Shasha Liu
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  2. Kecheng Zhu
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Correspondence to Huanling Wang.

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Liu, S., Zhu, K., Chen, N. et al. Identification of HIF-1α promoter and expression regulation of HIF-1α gene by LPS and hypoxia in zebrafish. Fish Physiol Biochem 39, 1153–1163 (2013). https://doi.org/10.1007/s10695-013-9771-0

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  • DOI: https://doi.org/10.1007/s10695-013-9771-0

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