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A new image of plantain diversity assessed by SSR, AFLP and MSAP markers

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Abstract

Using both SSR and AFLP markers, the genetic diversity of 30 plantains constituting a representative sample of the phenotypic diversity was assessed. The results confirmed a very narrow genetic base of this cultivar group. SSR and AFLP data support the hypothesis that these cultivars may have arisen from vegetative multiplication of a single seed. MSAP were used to survey cytosine methylation status at CCGG sites in order to obtain an alternative source of diversity data. A higher degree of polymorphism was revealed allowing the classification of the samples into three clusters. No correlation was observed between the phenotypic classification and methylation diversity. Implications for breeding programs are discussed.

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Abbreviations

AFLP:

Amplified Fragment Length Polymorphism

MSAP:

Methylation Sensitive Amplification Polymorphism

SSR:

Simple Sequence Repeat

RFLP:

Restriction Fragment Length Polymorphism

RAPD:

Random Amplified Polymorphic DNA

CARBAP:

Centre Africain de Recherches sur Bananiers et Plantains

CTAB:

Cetyltrimethylammoniumbromide

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Authors and Affiliations

  1. CIRAD, UMR 1096 PIA, France

    J. L. Noyer & A. Bouet

  2. CIRAD, UMR 1098 BEPC, TA 40/03, 34398, Montpellier cedex 5, France

    S. Causse & F. C. Baurens

  3. Centre Africain de Recherches sur Bananiers et Plantains (CARBAP), B.P. 832, Douala, Cameroun

    K. Tomekpe

Authors
  1. J. L. Noyer
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  2. S. Causse
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  3. K. Tomekpe
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  4. A. Bouet
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  5. F. C. Baurens
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Correspondence to J. L. Noyer.

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Noyer, J.L., Causse, S., Tomekpe, K. et al. A new image of plantain diversity assessed by SSR, AFLP and MSAP markers. Genetica 124, 61–69 (2005). https://doi.org/10.1007/s10709-004-7319-z

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  • DOI: https://doi.org/10.1007/s10709-004-7319-z

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