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FISH on avian lampbrush chromosomes produces higher resolution gene mapping

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Abstract

Giant lampbrush chromosomes, which are characteristic of the diplotene stage of prophase I during avian oogenesis, represent a very promising system for precise physical gene mapping. We applied 35 chicken BAC and 4 PAC clones to both mitotic metaphase chromosomes and meiotic lampbrush chromosomes of chicken (Gallus gallus domesticus) and Japanese quail (Coturnix coturnix japonica). Fluorescence in situ hybridization (FISH) mapping on lampbrush chromosomes allowed us to distinguish closely located probes and revealed gene order more precisely. Our data extended the data earlier obtained using FISH to chicken and quail metaphase chromosomes 1–6 and Z. Extremely low levels of inter- and intra-chromosomal rearrangements in the chicken and Japanese quail were demonstrated again. Moreover, we did not confirm the presence of a pericentric inversion in Japanese quail chromosome 4 as compared to chicken chromosome 4. Twelve BAC clones specific for chicken chromosome 4p and 4q showed the same order in quail as in chicken when FISH was performed on lampbrush chromosomes. The centromeres of chicken and quail chromosomes 4 seem to have formed independently after centric fusion of ancestral chromosome 4 and a microchromosome.

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Abbreviations

BAC:

bacterial artificial chromosome

CCO:

Coturnix coturnix japonica

cM:

centiMorgan, unit of genetic distance

FISH:

fluorescence in situ hybridization

GGA:

Gallus gallus domesticus

LBC:

lampbrush chromosome

Mb:

Megabase

PAC:

P1-derived artificial chromosome

PCR:

polymerase chain reaction

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Authors and Affiliations

  1. Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie shosse 2, Stary Peterhof, 198504, Saint-Petersburg, Russia

    Svetlana Galkina, Svetlana Deryusheva, Alexander Rodionov & Elena Gaginskaya

  2. Laboratoire de Genetique Cellulaire, Institut National de la Recherche Agronomique, Centre INRA de Toulouse, Toulouse, France

    Valerie Fillon & Alain Vignal

  3. Animal Breeding and Genetics group, Wageningen University, Wageningen, The Netherlands

    Richard Crooijmans & Martin Groenen

  4. The Komarov Botanical Institute, Russian Academy of Sciences, Saint-Petersburg, Russia

    Alexander Rodionov

Authors
  1. Svetlana Galkina
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  2. Svetlana Deryusheva
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  3. Valerie Fillon
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  4. Alain Vignal
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  5. Richard Crooijmans
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  6. Martin Groenen
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  7. Alexander Rodionov
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  8. Elena Gaginskaya
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Correspondence to Svetlana Deryusheva.

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Galkina, S., Deryusheva, S., Fillon, V. et al. FISH on avian lampbrush chromosomes produces higher resolution gene mapping . Genetica 128, 241–251 (2006). https://doi.org/10.1007/s10709-005-5776-7

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