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The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins

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Journal of Structural and Functional Genomics

Abstract

The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.

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Acknowledgments

We thank past and present colleagues who have participated in the development of the Protein Production Facility: Brandon Hillerich, Brian Kloss, Renato Bruni, Arianne Morrison, Patricia Rodriguez, Amanda Meyer, Jeff Bonanno, Zsolt Zolnai, Michael Weiner, Reinhard Grisshammer, and Gunnar von Heijne. This work was supported in part by a Cooperative Agreement from the NIGMS Protein Structure Initiative, U54GM075026.

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Authors and Affiliations

  1. NYCOMPS Core Laboratory, New York Structural Biology Center, New York, NY, 10027, USA

    James Love

  2. Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, 10032, USA

    Filippo Mancia & Ming Zhou

  3. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, 10032, USA

    Lawrence Shapiro, Marco Punta & Burkhard Rost

  4. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, NY, 10461, USA

    Mark Girvin

  5. Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, NY, 10016, USA

    Da-Neng Wang

  6. Department of Biological Sciences, Columbia University, New York, NY, 10027, USA

    John F. Hunt

  7. Department of Chemistry, University of Buffalo, Buffalo, NY, 14260, USA

    Thomas Szyperski

  8. Howard Hughes Medical Institute, The Vollum Institute, Oregon Health & Science University, Portland, OR, 97239, USA

    Eric Gouaux

  9. Howard Hughes Medical Institute, The Rockefeller University, New York, NY, 10021, USA

    Roderick MacKinnon

  10. Department of Chemistry, Columbia University, New York, NY, 10027, USA

    Ann McDermott

  11. Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, 10032, USA

    Barry Honig & Wayne A. Hendrickson

  12. Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA

    Masayori Inouye

  13. Department of Biochemistry, UMDNJ, Piscataway, NJ, 08854, USA

    Gaetano Montelione

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  1. James Love
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  2. Filippo Mancia
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  14. Barry Honig
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Corresponding author

Correspondence to Wayne A. Hendrickson.

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Love, J., Mancia, F., Shapiro, L. et al. The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins. J Struct Funct Genomics 11, 191–199 (2010). https://doi.org/10.1007/s10969-010-9094-7

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  • DOI: https://doi.org/10.1007/s10969-010-9094-7

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