Abstract
Two wheat–Thinopyrum substitution lines X479 and X482 selected from the progenies of wheat “Mianyang26 (MY26)” × wheat–Thinopyrum intermedium ssp. trichophorum partial amphiploid were characterized by seed storage protein electrophoresis, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and PCR-based molecular markers. Seed storage protein analysis showed that X479 expressed some of Th. intermedium ssp. trichophorum-specific gliadin and glutenin bands. Chromosome counting and GISH probed by Pseudoroegneria spicata genomic DNA indicated that two pairs of Thinopyrum-derived chromosomes (St genome and St–JS translocated chromosomes) substituted for two pairs of wheat chromosomes in both X479 and X482. FISH using pAs1 and pHvG38 as probes showed that chromosomes 1B and 4B, and 4D and 6D were absent in X479 and X482, respectively. Using the newly isolated JS chromosome-specific repetitive sequence pDb12H as a probe, the FISH signals revealed that the translocation of St–JS chromosomes in X479 and X482 occurred in repetitive sequence regions of the short arm. The molecular markers based on wheat–rice colinearity confirmed that the chromosome constitutions of X479 and X482 were 1St (1B) + 4St–4JS (4B) and 4St–JS (4D) + 6St (6D), respectively. The substitution lines were both fully fertile which suggests that the Th. intermedium chromosomes in X479 and X482 substitute well for the corresponding wheat chromosomes. The rust resistance and novel agronomic traits revealed that the substitution lines will be potentially useful for genetic improvement of wheat.
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Acknowledgments
We particularly thank Dr. I. Dundas at University of Adelaide, Australia, for reviewing the manuscript. We thank the National Natural Science Foundation of China (Nos. 31101143, 31171542, 31201203), and Sichuan Wheat Breeding Community for the financial support.
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Li, G., Lang, T., Dai, G. et al. Precise identification of two wheat–Thinopyrum intermedium substitutions reveals the compensation and rearrangement between wheat and Thinopyrum chromosomes. Mol Breeding 35, 1 (2015). https://doi.org/10.1007/s11032-015-0202-z
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DOI: https://doi.org/10.1007/s11032-015-0202-z