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The isolation, characterization and antifungal susceptibilities of Cryptococcus neoformans from bird excreta in Klang Valley, Malaysia

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Abstract

The occurrence of Cryptococcus neoformans in bird excreta in Klang valley, Malaysia was determined in this study. Of 544 samples of bird excreta collected from a local zoo, pet shops and public areas, 20 strains of C. neoformans were isolated. All C. neoformans strains were serotype A and thus identified as C. neoformans variety grubii. All did not produce color changes on canavanine–glycine–bromothymol blue agar. All were of α-mating types, as determined by a pheromone-specific PCR assay. The antifungal susceptibility testing using agar diffusion method Neo-sensitabs showed that all were susceptible to amphotericin B, fluconazole and itraconazole.

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References

  1. TG Mitchell JR Perfect (1995) ArticleTitleCryptococcosis in the era of AIDS – 100 years after the discovery of Cryptococcus neoformans Clin Microbiol Rev 8 515–548 Occurrence Handle1:STN:280:BymC3MvitFQ%3D Occurrence Handle8665468

    CAS  PubMed  Google Scholar 

  2. SP Franzot IF Salkin A Casadevall (1999) ArticleTitleCryptococcus neoformans var. grubii separate varietal status for Cryptococcus neoformans serotype A isolates J Clin Microbiol 37 838–840 Occurrence Handle1:STN:280:DyaK1M7ktlaluw%3D%3D Occurrence Handle9986871

    CAS  PubMed  Google Scholar 

  3. F Dromer S Mathoulin B Dupont L Letenneur O Ronin (1996) ArticleTitleIndividual and environmental factors associated with infection due to Cryptococcus neoformans serotype D. French Cryptococcosis Study Group Clin Infect Dis 23 91–96 Occurrence Handle1:STN:280:BymH3M3ovV0%3D Occurrence Handle8816135

    CAS  PubMed  Google Scholar 

  4. TC Sorrell (2001) ArticleTitleCryptococcus neoformans variety gattii Med Mycol 39 155–168 Occurrence Handle1:STN:280:DC%2BD3M3ltVCksQ%3D%3D Occurrence Handle11346263

    CAS  PubMed  Google Scholar 

  5. KJ Kwon-Chung T Boekhout JW Fell M Diaz (2002) ArticleTitle(1557) Proposal to conserve the name Cryptococcus gattii against C. hondurianus and C. bacillisporus (Basidiomycota, Hymenomycetes, Tremellomycetidae) Taxon 51 804–806

    Google Scholar 

  6. T Boekhout B Theleen M Diaz JW Fell WCJ Hop ECA Abeln F Dromer W Meyer (2001) ArticleTitleHybrid genotypes in the pathogenic yeast Cryptococcus neoformans Microbiology 147 891–907 Occurrence Handle1:CAS:528:DC%2BD3MXjtVeisLw%3D Occurrence Handle11283285

    CAS  PubMed  Google Scholar 

  7. KJ Kwon-Chung JC Edman BL Wickes (1992) ArticleTitleGenetic association of mating types and virulence in Cryptococcus neoformans Infect Immun 60 602–605 Occurrence Handle1:STN:280:By2C3M3jt1I%3D Occurrence Handle1730495

    CAS  PubMed  Google Scholar 

  8. N Poonwan Y Mikami S Poosuwan J Boon-Long N Mekha M Kusum K Yazawa R Tanaka K Nishimura K Konyama (1997) ArticleTitleSerotyping of Cryptococcus neoformans strains isolated from clinical specimens in Thailand and their susceptibility to various antifungal agents Eur J Epidemiol 13 335–340 Occurrence Handle10.1023/A:1007376917836 Occurrence Handle1:CAS:528:DyaK2sXlsVertr8%3D Occurrence Handle9258534

    Article  CAS  PubMed  Google Scholar 

  9. B Sar D Monchy M Vann C Keo JL Sarthou Y Buisson (2004) ArticleTitleIncreasing in vitro resistance to fluconazole in Cryptococcus neoformans Cambodian isolates: April 2000 to March 2002 J Antimicrob Chemother 54 563–565 Occurrence Handle10.1093/jac/dkh361 Occurrence Handle1:CAS:528:DC%2BD2cXnt1Wit7g%3D Occurrence Handle15254027

    Article  CAS  PubMed  Google Scholar 

  10. MB Taylor D Chadwick T Barkham (2002) ArticleTitleFirst reported isolation of Cryptococcus neoformans var. gattii from a patient in Singapore J Clin Microbiol 40 3098–3099 Occurrence Handle10.1128/JCM.40.8.3098-3099.2002 Occurrence Handle1:STN:280:DC%2BD38vgtVWktA%3D%3D Occurrence Handle12149391

    Article  CAS  PubMed  Google Scholar 

  11. S Sukroongreung C Nilakul O Ruangsomboon W Chuakul B Eampokalap (1996) ArticleTitleSerotypes of Cryptococcus neoformans isolated from patients prior to and during the AIDS era in Thailand Mycopathologia 135 75–78 Occurrence Handle10.1007/BF00436454 Occurrence Handle1:STN:280:ByiB3MnosV0%3D Occurrence Handle9063001

    Article  CAS  PubMed  Google Scholar 

  12. R Pathmanathan Soon Soo-Hoo Tuck (1982) ArticleTitleCryptococcosis in the University Hospital, Kuala Lumpur and review of published cases Trans R Soc Trop Med Hyg 76 21–24 Occurrence Handle10.1016/0035-9203(82)90008-6 Occurrence Handle1:STN:280:Bi2B3c%2Fkt1w%3D Occurrence Handle7080152

    Article  CAS  PubMed  Google Scholar 

  13. PM Richardson A Mohandas N Arumugasamy (1976) ArticleTitleCerebral cryptococcosis in Malaysia J Neurol Neurosurg Psychiatry 39 330–337 Occurrence Handle1:STN:280:CSmB3MnmsF0%3D Occurrence Handle932751

    CAS  PubMed  Google Scholar 

  14. V Nissapatorn C Lee QK Fatt KA Abdullah (2003) ArticleTitleAIDS-related opportunistic infections in Hospital Kuala Lumpur Jpn J Infect Dis 56 187–192 Occurrence Handle14695428

    PubMed  Google Scholar 

  15. SM Said TS Soo-HOO T Pang (1993) ArticleTitleSerotypes of Cryptococcus neoformans isolated in Malaysia over a 11-year period Trop Biomed 10 191–192

    Google Scholar 

  16. KC Keah S Parameswari YM Cheong (1994) ArticleTitleSerotypes of clinical isolates of Cryptococcus neoformans in Malaysia Trop Biomed 11 205–207

    Google Scholar 

  17. ST Yildiran MA Saracli AW Fothergill MG Rinaldi (2000) ArticleTitleIn vitro susceptibility of environmental Cryptococcus neoformans variety neoformans isolates from Turkey to six antifungal agents, including SCH56592 and voriconazole Eur J Clin Microbiol Infect Dis 19 317–319 Occurrence Handle10.1007/s100960050484 Occurrence Handle1:CAS:528:DC%2BD3cXkt1Gisr0%3D Occurrence Handle10834825

    Article  CAS  PubMed  Google Scholar 

  18. I Polacheck (1991) ArticleTitleThe discovery of melanin production in Cryptococcus neoformans and its impact on diagnosis and the study of virulence Zentralbl. Bakteriol 276 120–123 Occurrence Handle1:STN:280:By2C283hsVY%3D Occurrence Handle1789897

    CAS  PubMed  Google Scholar 

  19. KJ Kwon-Chung I Polacheck JE Bennett (1982) ArticleTitleImproved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C) J Clin Microbiol 15 535–537 Occurrence Handle1:STN:280:Bi2C1M%2FjtlY%3D Occurrence Handle7042750

    CAS  PubMed  Google Scholar 

  20. S Chaturvedi B Rodeghier J Fan CM McClelland BL Wickes V Chaturvedi (2000) ArticleTitleDirect PCR of Cryptococcus neoformans MATα and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies J Clin Microbiol 38 2007–2009 Occurrence Handle1:CAS:528:DC%2BD3cXjsFegt78%3D Occurrence Handle10790143

    CAS  PubMed  Google Scholar 

  21. P Sriburee S Khayhan C Khamwan S Panjaisee P Tharavichitkul (2004) ArticleTitleSerotype and PCR-fingerprints of clinical and environmental isolates of Cryptococcus neoformans in Chiang Mai, Thailand Mycopathologia 158 25–31 Occurrence Handle1:CAS:528:DC%2BD2cXmslCms70%3D Occurrence Handle15487316

    CAS  PubMed  Google Scholar 

  22. M Abraham MS Matthews TJ John (1997) ArticleTitleEnvironmental isolation of Cryptococcus neoformans var. neoformans from Vellore Indian J Med Res 106 458–459 Occurrence Handle1:STN:280:DyaK1c%2FnslGrsw%3D%3D Occurrence Handle9415740

    CAS  PubMed  Google Scholar 

  23. A Li K Nishimura H Taguchi R Tanaka S Wu M Miyaji (1993) ArticleTitleThe isolation of Cryptococcus neoformans from pigeon droppings and serotyping of naturally and clinically sourced isolates in China Mycopathologia 124 1–5 Occurrence Handle10.1007/BF01103049 Occurrence Handle1:STN:280:ByuB3M7is1Q%3D Occurrence Handle8159213

    Article  CAS  PubMed  Google Scholar 

  24. A Weber R Schafer (1991) ArticleTitleThe occurrence of Cryptococcus neoformans in fecal samples from birds kept in human living areas Berl Munch Tierarztl Wochenschr 104 419–421 Occurrence Handle1:STN:280:By2C2MzhslE%3D Occurrence Handle1786044

    CAS  PubMed  Google Scholar 

  25. G Criseo MS Bolignano F De Leo F Staib (1995) ArticleTitleEvidence of canary droppings as an important reservoir of Cryptococcus neoformans Zentralbl Bakteriol 282 244–254 Occurrence Handle1:STN:280:BymD3c3hs10%3D Occurrence Handle7549156

    CAS  PubMed  Google Scholar 

  26. U Kumlin B Olsen M Granlund LG Elmqvist A Tarnvik (1998) ArticleTitleCryptococcosis and starling nests Lancet 351 1181 Occurrence Handle10.1016/S0140-6736(05)79126-1 Occurrence Handle1:STN:280:DyaK1czgsFymsA%3D%3D

    Article  CAS  Google Scholar 

  27. JD Nosanchuk S Shoham BC Fries DS Shapiro S Levitz A Casadevall (2000) ArticleTitleEvidence of zoonotic transmission of Cryptococcus neoformans from a pet cockatoo to an immunocompromised patient Ann Intern Med 132 205–208 Occurrence Handle1:STN:280:DC%2BD3c7gsVyjsA%3D%3D Occurrence Handle10651601

    CAS  PubMed  Google Scholar 

  28. M Kuroki C Phichaichumpon A Yasuoka P Chiranairadul T Chosa P Sirinirund T Miyazaki H Kakeya Y Higashiyama Y Miyazaki Y Ishida S Kohno (2004) ArticleTitleEnvironmental isolation of Cryptococcus neoformans from an endemic region of HIV-associated cryptococcosis in Thailand Yeast 21 809–812 Occurrence Handle10.1002/yea.1112 Occurrence Handle1:CAS:528:DC%2BD2cXntVantb8%3D Occurrence Handle15300677

    Article  CAS  PubMed  Google Scholar 

  29. D Garcia-Hermoso S Mathoulin-Pelissier B Couprie O Ronin B Dupont F Dromer (1997) ArticleTitleDNA typing suggests pigeon droppings as a source of pathogenic Cryptococcus neoformans serotype D J Clin Microbiol 35 2683–2685 Occurrence Handle1:STN:280:ByiH287isFE%3D Occurrence Handle9316936

    CAS  PubMed  Google Scholar 

  30. ME Brandt MA Pfaller RA Hajjeh RJ Hamill PG Pappas AL Reingold D Rimland DW Warnock (2001) ArticleTitleCryptococcal Disease Active Surveillance Group. Trends in antifungal drug susceptibility of Cryptococcus neoformans isolates in the United States: 1992 to 1994 and 1996 to 1998 Antimicrob Agents Chemother 45 3065–3069 Occurrence Handle10.1128/AAC.45.11.3065-3069.2001 Occurrence Handle1:CAS:528:DC%2BD3MXnvF2jsb4%3D Occurrence Handle11600357

    Article  CAS  PubMed  Google Scholar 

  31. SP Franzot JS Hamdan (1996) ArticleTitleIn vitro susceptibilities of clinical and environmental isolates of Cryptococcus neoformans to five antifungal drugs Antimicrob Agents Chemother 40 822–824 Occurrence Handle1:CAS:528:DyaK28XhsVersro%3D Occurrence Handle8851624

    CAS  PubMed  Google Scholar 

  32. SH Alves LT Oliveira JM Costa I Lubeck AK Casali MH Vainstein (2001) ArticleTitleIn vitro susceptibility to antifungal agents of clinical and environmental Cryptococcus neoformans isolated in southern of Brazil Rev Inst Med Trop Sao Paulo 43 267–270 Occurrence Handle1:STN:280:DC%2BD3MnksVCksg%3D%3D Occurrence Handle11696849

    CAS  PubMed  Google Scholar 

  33. WJ Fessel (1993) ArticleTitleCryptococcal meningitis after unusual exposures to birds N Engl J Med 328 1354–1355 Occurrence Handle10.1056/NEJM199305063281816 Occurrence Handle1:STN:280:ByyB38ngt1U%3D Occurrence Handle8469264

    Article  CAS  PubMed  Google Scholar 

  34. HG Muchmore ER Rhoades GE Nix FG Felton RE Carpenter (1963) ArticleTitleOccurrence of Cryptococcus neoformans in the environment of three geographically associated cases of cryptococcal meningitis N Engl J Med 268 1112–1114 Occurrence Handle1:STN:280:CC2C38fhtF0%3D Occurrence Handle13936450

    CAS  PubMed  Google Scholar 

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Tay, S.T., Chai, H.C., Na, S.L. et al. The isolation, characterization and antifungal susceptibilities of Cryptococcus neoformans from bird excreta in Klang Valley, Malaysia. Mycopathologia 159, 509–513 (2005). https://doi.org/10.1007/s11046-005-3091-6

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  • DOI: https://doi.org/10.1007/s11046-005-3091-6

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