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Analysis of the variability of CMV strains in the RL11D domain of the RL11 multigene family

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Abstract

Human cytomegalovirus (CMV) contains one of the largest genomes within the herpesvirus family and includes 12 multigene families. One of these is the RL11 family, whose members encode a characteristic domain, called RL11D. In the present study, the sequence variability of RL11D within the UL1, UL4, UL6, UL7, and UL10 genes of the RL11 family was investigated. For this purpose, these genes were analyzed in 70 clinical isolates obtained from urine, bronchoalveolar lavage, and blood of different patients. Substantial genetic variability among the clinical isolates was observed in all five genes analyzed. Based on phylogenetic analysis of variations in RL11D, the clinical isolates could be classified into three genotypes for UL1, 7, and 10 and, four genotypes for UL4 and 6. Further analysis showed statistically significant linkages between the following pairs of genes: UL6/UL7, UL4/UL7, UL1/UL4, and UL4/UL6. The data show that CMV strains exhibit a high interstrain variability in the RL11D domain of various RL11 family genes. Sequence variations, however, can be clearly grouped into a limited number of distinct genotypes. The genetic linkages found probably reflect a low frequency of recombination between genes that are arranged in close proximity on the viral genome.

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References

  1. M.S. Chee, A.T. Bankier, S. Beck et al., Curr. Top. Microbiol. Immunol. 154, 125–169 (1990)

    CAS  PubMed  Google Scholar 

  2. A. Dolan, C. Cunningham, R.D. Hector, A.F. Hassan-Walker, L. Lee, C. Addison, D.J. Dargan, D.J. McGeoch, D. Gatherer, V.C. Emery, P.D. Griffiths, C. Sinzger, B.P. McSharry, G.W.G. Wilkinson, A.J. Davison, J. Gen. Virol. 85, 1301–1312 (2004)

    Article  CAS  PubMed  Google Scholar 

  3. E. Murphy, D. Yu, J. Grimwood, J. Schmutz, M. Dickson, M.A. Jarvis, G. Hahn, J.A. Nelson, R.M. Myers, T.E. Shenk, Proc. Natl. Acad. Sci. USA 100, 14976–14981 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. S. Chou, K.M. Dennison, J. Infect. Dis. 163, 1229–1234 (1991)

    Article  CAS  PubMed  Google Scholar 

  5. S. Pignatelli, P. Dal Monte, G. Rossini, S. Chou, T. Gojobori, K. Hanada, J.J. Guo, W. Rawlinson, W. Britt, M. Mach, M.P. Landini, J. Gen. Virol. 84, 647–655 (2003)

    Article  CAS  PubMed  Google Scholar 

  6. L. Rasmussen, A. Geissler, C. Cowan, A. Chase, M. Winters, J. Virol. 76, 10841–10848 (2002)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. E.R. Kinzler, T. Compton, J. Virol. 79, 7827–7837 (2005)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. R. Arav-Boger, R.E. Willoughby, R.F. Pass, J.C. Zong, W.J. Jang, D. Alcendor, G.S. Hayward, J. Infect. Dis. 186, 1057–1064 (2002)

    Article  CAS  PubMed  Google Scholar 

  9. L. Rasmussen, A. Geissler, M. Winters, J. Infect. Dis. 187, 809–819 (2003)

    Article  PubMed  Google Scholar 

  10. C. Mattick, D. Dewin, S. Polley, E. Sevilla-Reyes, S. Pignatelli, W. Rawlinson, G. Wilkinson, P. Dal Monte, U.A. Gompels, Virology 318, 582–597 (2004)

    Article  CAS  PubMed  Google Scholar 

  11. A.J. Davison, A. Dolan, P. Akter, C. Addison, D.J. Dargan, D.J. Alcendor, D.J. McGeoch, G.S. Hayward, J. Gen. Virol. 84, 17–28 (2003)

    Article  CAS  PubMed  Google Scholar 

  12. A.J. Davison, P. Akter, C. Cunningham, A. Dolan, C. Addison, D.J. Dargan, A.F. Hassan-Walker, V.C. Emery, P.D. Griffiths, G.W.G. Wilkinson, J. Gen. Virology 84, 657–663 (2003)

    Article  CAS  Google Scholar 

  13. F. Deryckere, H.G. Burgert, J. Virol. 70, 2832–2841 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  14. I. Rigoutsos, J. Novotny, T. Huynh, S.T. Chin-Bow, L. Parida, D. Platt, D. Coleman, T. Shenk, J. Virol. 77, 4326–4344 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. U. Hobom, W. Brune, M. Messerle, G. Hahn, U.H. Koszinowski, J. Virol. 74, 7720–7729 (2000)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. W.D. Rawlinson, H.E. Farrell, B.G. Barrell, J. Virol. 70, 8833–8849 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  17. J.P. Alderete, S. Jarrahian, A.P. Geballe, J. Virol. 73, 8330–8337 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  18. W. Dunn, C. Chou, H. Li, R. Hai, D. Patterson, V. Stolc, H. Zhu, F. Liu, Proc. Natl. Acad. Sci. USA 100, 14223–14228 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. S.W. Aberle, E. Puchhammer-Stöckl, J. Clin. Virol. 25(Suppl 1), S79–S85 (2002)

    Article  CAS  PubMed  Google Scholar 

  20. J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 25, 4876–4882 (1997)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. T.A. Hall, Nucleic Acids Symp. Ser. 41, 95–98 (1999)

    CAS  Google Scholar 

  22. D. Posada, K.A. Crandall, Bioinformatics 14, 817–818 (1998)

    Article  CAS  PubMed  Google Scholar 

  23. S. Kumar, K. Tamura, I.B. Jakobsen, M. Nei, MEGA2. (Arizona state University, Tempe, Arizona, USA, 2001)

    Google Scholar 

  24. E. Puchhammer-Stöckl, I. Görzer, A. Zoufaly, P. Jaksch, C.C. Bauer, W. Klepetko, T. Popow-Kraupp, Transplantation 81, 187–194 (2006)

    Article  PubMed  Google Scholar 

  25. H. Chernoff, E.L. Lehmann, Ann. Math. Stat. 25, 579–586 (1954)

    Article  Google Scholar 

  26. S. Chou, J. Infect. Dis. 166, 604–607 (1992)

    Article  CAS  PubMed  Google Scholar 

  27. M. Bar, C. Shannon-Lowe, A.P. Geballe, J. Infect. Dis. 183, 218–225 (2001)

    Article  CAS  PubMed  Google Scholar 

  28. J. Novotny, I. Rigoutsos, D. Coleman, T. Shenk, J. Mol. Biol. 310, 1151–1166 (2001)

    Article  CAS  PubMed  Google Scholar 

  29. S. Watanabe, M. Shinkai, S. Hitomi, H. Kozuka, S. Kimura, K. Shimada, R. Hondo, N. Yamaguchi, Arch. Virol. 137, 117–121 (1994)

    Article  CAS  PubMed  Google Scholar 

  30. L. Prix, R. Kuner, C.P. Speer, G. Jahn, K. Hamprecht, Eur. J. Clin. Microbiol. Infect. Dis. 17, 525–528 (1998)

    Article  CAS  PubMed  Google Scholar 

  31. D. Yu, M.C. Silva, T. Shenk, Proc. Natl. Acad. Sci. USA 100, 12396–12401 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. K. Umene, Rev. Med. Virol. 9, 171–182 (1999)

    Article  CAS  PubMed  Google Scholar 

  33. C. Steininger, B. Schmied, M. Sarcletti, M. Geit, E. Puchhammer-Stöckl, AIDS 19, 273–278 (2005)

    CAS  PubMed  Google Scholar 

  34. V.H. Aquino, L.T.M. Figueiredo, J. Med. Virol. 61, 138–142 (2000)

    Article  CAS  PubMed  Google Scholar 

  35. U. Meyer-Konig, K. Ebert, B. Schrage, S. Pollak, F.T. Hufert, Lancet 352, 1280–1281 (1998)

    Article  CAS  PubMed  Google Scholar 

  36. B.C. Fries, S. Chou, M. Boeckh, B. Torok-Storb, J. Infect. Dis. 169, 769–774 (1994)

    Article  CAS  PubMed  Google Scholar 

  37. D. Tarrago, C. Quereda, A. Tenorio, J. Clin. Microbiol. 41, 2872–2877 (2003)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgment

We thank Claudia Kellner for her excellent technical assistance.

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Authors and Affiliations

  1. Institute of Virology, Medical University of Vienna, Kinderspitalgasse 15, Vienna, 1095, Austria

    Karin Sekulin, Irene Görzer & Elisabeth Puchhammer-Stöckl

  2. Austrian Research Centers, Vienna, Austria

    Dorothea Heiss-Czedik

Authors
  1. Karin Sekulin
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  2. Irene Görzer
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  3. Dorothea Heiss-Czedik
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  4. Elisabeth Puchhammer-Stöckl
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Correspondence to Elisabeth Puchhammer-Stöckl.

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Sekulin, K., Görzer, I., Heiss-Czedik, D. et al. Analysis of the variability of CMV strains in the RL11D domain of the RL11 multigene family. Virus Genes 35, 577–583 (2007). https://doi.org/10.1007/s11262-007-0158-0

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  • DOI: https://doi.org/10.1007/s11262-007-0158-0

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