Skip to main content

Advertisement

SpringerLink
Log in

CD10 Expression in Epithelial and Stromal Cells of Non-small Cell Lung Carcinoma (NSCLC): A Clinic and Pathologic Correlation

  • Research
  • Published:
Pathology & Oncology Research

Abstract

CD10 is a zinc dependent metallopeptidase, and its expression in stromal and/or epithelial cells of many carcinomas has been suggested to have prognostic value. This study investigates CD10 expression in epithelial and stromal cells of non small cell lung carcinoma (NSCLC), and evaluates its prognostic value for this tumor and its histologic subtypes. Sixty-six cases of NSCLC [35 cases of nonsquamous cell carcinoma (NSCC) and 31 cases of squamous cell carcinoma (SCC)] were analyzed immuno-histochemically for CD10 antibody. Fisher’s exact test and univariate survival analyses were performed. Comparison of clinicopathologic characteristics for NSCLC showed that only stromal CD10 expression had worse prognostic impact, associated with the presence of recurrence (p = 0.001), death (p = 0.006) and disease positivity (p = 0.001). For SCC, CD10 was found to be expressed mainly in the stromal cells, and was associated with a decreased survival (p = 0.000) and disease free survival (p = 0.000). CD10 expression was restricted to the epithelial cells in NSCC and associated with an increased disease free survival (p = 0.036). Stromal CD10 expression apppears to be a worse prognostic factor in NSCLCs. CD10 which is expressed in different cell components of SCC and NSCC appears to have opposing effects on the behaviour of these histologic types.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

NSCLC:

Nonsmall cell lung carcinoma

NSCC:

Nonsquamous cell carcinoma

SCC:

Squamous cell carcinoma

References

  1. Shipp MA, Vijayaraghavan J, Schmidt EV et al (1989) Common acute lymphoblastic leukemia antigen (CALLA) is active neutral endopeptidase 24.11 (“enkephalinase”): direct evidence by cDNA transfection analysis. Proc Natl Acad Sci USA 86:297–301

    Article  PubMed  CAS  Google Scholar 

  2. Borscheri N, Roessner A, Röcken C (2001) Canalicular immunostaining of neprilysin (CD10) as a diagnostic marker for hepatocellular carcinomas. Am J Surg Pathol 25:1297–1303

    Article  PubMed  CAS  Google Scholar 

  3. Xiao SY, Wang HL, Hart J et al (2001) cDNA arrays and immunohistochemistry identification of CD10/CALLA expression in hepatocellular carcinoma. Am J Pathol 159:1415–1421

    Article  PubMed  CAS  Google Scholar 

  4. Xu Y, McKenna RW, Molberg KH et al (2001) Clinicopathologic analysis of CD10+ and CD10- diffuse large B-cell lymphoma. Identification of a high-risk subset with coexpression of CD10 and bcl-2. Am J Clin Pathol 116(2):183–190

    Article  PubMed  CAS  Google Scholar 

  5. Xu Y, McKenna RW, Kroft SH (2002) Comparison of multiparameter flow cytometry with cluster analysis and immunohistochemistry for the detection of CD10 in diffuse large B-Cell lymphomas. Mod Pathol 15:413–419

    Article  PubMed  Google Scholar 

  6. Albrecht M, Gillen S, Wilhelm B et al (2002) Expression, localization and activity of neutral endopeptidase in cultured cells of benign prostatic hyperplasia and prostate cancer. J Urol 168:336–342

    Article  PubMed  CAS  Google Scholar 

  7. Chu P, Arber DA (2000) Paraffin-section detection of CD10 in 505 nonhematopoietic neoplasms. Frequent expression in renal cell carcinoma and endometrial stromal sarcoma. Am J Clin Pathol 113:374–382

    Article  PubMed  CAS  Google Scholar 

  8. Moreau I, Duvert V, Caux C et al (1993) Myofibroblastic stromal cells isolated from human bone marrow induce the proliferation of both early myeloid and B-lymphoid cells. Blood 82:2396–2405

    PubMed  CAS  Google Scholar 

  9. Imai K, Kanzaki H, Fujiwara H et al (1992) Expression of aminopeptidase N and neutral endopeptidase on the endometrial stromal cells in endometriosis and adenomyosis. Hum Reprod 7:1326–1328

    PubMed  CAS  Google Scholar 

  10. Shipp MA, Tarr GE, Chen CY et al (1991) CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung. Proc Natl Acad Sci USA 88:10662–10666

    Article  PubMed  CAS  Google Scholar 

  11. Göhring B, Holzhausen HJ, Meye A et al (1998) Endopeptidase 24.11/CD10 is down-regulated in renal cell cancer. Int J Mol Med 2:409–414

    PubMed  Google Scholar 

  12. Koiso K, Akaza H, Ohtani M et al (1994) A new tumor marker for bladder cancer. Int J Urol 1:33–36

    Article  PubMed  CAS  Google Scholar 

  13. Sato Y, Itoh F, Hinoda Y et al (1996) Expression of CD10/neutral endopeptidase in normal and malignant tissues of the human stomach and colon. J Gastroenterol 31:12–17

    Article  PubMed  CAS  Google Scholar 

  14. Cohen AJ, Bunn PA, Franklin W et al (1996) Neutral endopeptidase: variable expression in human lung, inactivation in lung cancer, and modulation of peptide-induced calcium flux. Cancer Res 56:831–839

    PubMed  CAS  Google Scholar 

  15. Cohen AJ, Gilman LB, Moore M et al (1996) Inactivation of neutral endopeptidase in lung cancer. Chest 109:12–13

    Article  Google Scholar 

  16. Tawfic S, Niehans GA, Manivel JC (2003) The pattern of CD10 expression in selected pathologic entities of the prostate gland. Hum Pathol 34:450–456

    Article  PubMed  Google Scholar 

  17. Bilalovic N, Sandstad B, Golouh R et al (2004) CD10 protein expression in tumor and stromal cells of malignant melanoma is associated with tumor progression. Mod Pathol 17:1251–1258

    Article  PubMed  CAS  Google Scholar 

  18. Ogawa H, Iwaya K, Izumi M et al (2002) Expression of CD10 by stromal cells during colorectal tumor development. Hum Pathol 33:806–811

    Article  PubMed  Google Scholar 

  19. Huang WB, Zhou XJ, Chen JY et al (2005) CD10-positive stromal cells in gastric carcinoma: correlation with invasion and metastasis. Jpn J Clin Oncol 35:245–250

    Article  PubMed  Google Scholar 

  20. Braham H, Trimeche M, Ziadi S et al (2006) CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows Arch 449:220–224

    Article  PubMed  CAS  Google Scholar 

  21. Aiad HA, Hanout HM (2007) Immunohistochemical expression of CD10 in cutaneous basal and squamous cell carcinomas. J Egypt Natl Canc Inst 19:195–201

    PubMed  Google Scholar 

  22. Piattelli A, Fioroni M, Iezzi G et al (2006) CD10 expression in stromal cells of oral cavity squamous cell carcinoma: a clinic and pathologic correlation. Oral Dis 12:301–304

    Article  PubMed  CAS  Google Scholar 

  23. Yada K, Kashima K, Daa T et al (2004) Expression of CD10 in basal cell carcinoma. Am J Dermatopathol 26:463–471

    Article  PubMed  Google Scholar 

  24. Iwaya K, Ogawa H, Izumi M et al (2002) Stromal expression of CD10 in invasive breast carcinoma: a new predictor of clinical outcome. Virchows Arch 440:589–593

    Article  PubMed  CAS  Google Scholar 

  25. Kristiansen G, Schlüns K, Yongwei Y et al (2002) CD10 expression in non-small cell lung cancer. Anal Cell Pathol 24:41–46

    PubMed  CAS  Google Scholar 

  26. Tokuhara T, Adachi M, Hashida H et al (2001) Neutral endopeptidase/CD10 and aminopeptidase N/CD13 gene expression as a prognostic factor in non-small cell lung cancer. Jpn J Thorac Cardiovasc Surg 49:489–496

    Article  PubMed  CAS  Google Scholar 

  27. Kajiyama H, Shibata K, Terauchi M et al (2005) Neutral endopeptidase 24.11/CD10 suppresses progressive potential in ovarian carcinoma in vitro and in vivo. Clin Cancer Res 11:1798–1808

    Article  PubMed  CAS  Google Scholar 

  28. Ganju RK, Sunday M, Tsarwhas DG et al (1994) CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation. J Clin Invest 94:1784–1791

    Article  PubMed  CAS  Google Scholar 

  29. Mountain CF (1997) Revisions in the international system for staging lung cancer. Chest 111:1710–1717

    Article  PubMed  CAS  Google Scholar 

  30. Johnson AR, Ashton J, Schulz WW et al (1985) Neutral metalloendopeptidase in human lung tissue and cultured cells. Am Rev Respir Dis 132:564–568

    PubMed  CAS  Google Scholar 

  31. Makretsov NA, Hayes M, Carter BA et al (2007) Stromal CD10 expression in invasive breast carcinoma correlates with poor prognosis, estrogen receptor negativity, and high grade. Mod Pathol 20:84–89

    Article  PubMed  CAS  Google Scholar 

  32. Basset P, Wolf C, Rouyer N et al (1994) Stromelysin-3 in stromal tissue as a control factor in breast cancer behavior. Cancer 74:1045–1049

    Article  PubMed  CAS  Google Scholar 

  33. Talvensaari-Mattila A, Pääkkö P, Höyhtyä M et al (1998) Matrix metalloproteinase-2 immunoreactive protein: a marker of aggressiveness in breast carcinoma. Cancer 83:1153–1162

    Article  PubMed  CAS  Google Scholar 

  34. Deschamps L, Handra-Luca A, O’Toole D et al (2006) CD10 expression in pancreatic endocrine tumors: correlation with prognostic factors and survival. Hum Pathol 37:802–808

    Article  PubMed  CAS  Google Scholar 

  35. Abdou AG (2007) CD10 expression in tumour and stromal cells of bladder carcinoma: an association with bilharziasis. APMIS 115:1206–1218

    Article  PubMed  Google Scholar 

  36. Sumitamo M, Shen R, Walburg M et al (2000) Neutral endopeptidase inhibits prostate cancer cell migration by blocking focal adhesion kinase signalling. J Clin Invest 106:1399–1407

    Article  Google Scholar 

  37. Kletsas D, Caselgrandi E, Barbieri D et al (1998) Neutral endopeptidase-24.11 (NEP) activity in human fibroblasts during development and ageing. Mech Ageing Dev 102:15–23

    Article  PubMed  CAS  Google Scholar 

  38. Kondepudi A, Johnson A (1993) Cytokines increase neutral endopeptidase activity in lung fibroblasts. Am J Respir Cell Mol Biol 8:43–49

    PubMed  CAS  Google Scholar 

  39. Casey ML, Smith JW, Nagai K et al (1993) Transforming growth factor-beta 1 inhibits enkephalinase (EC 3.4.24.11) gene expression in human endometrial stromal cells and sex skin fibroblasts in culture. J Clin Endocrinol Metab 77:144–150

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, Dokuz Eylül University, İzmir, Turkey

    Duygu Gürel, Aydanur Kargı, Ilgın Karaman & Mehtat Ünlü

  2. Department of Thoracic Surgery, Faculty of Medicine, Dokuz Eylül University, Izmir, Turkey

    Ahmet Önen

  3. Pathology Department, School of Medicine, Dokuz Eylül University, Inciraltı, Izmir, Turkey

    Duygu Gürel

Authors
  1. Duygu Gürel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aydanur Kargı
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ilgın Karaman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ahmet Önen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mehtat Ünlü
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Duygu Gürel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gürel, D., Kargı, A., Karaman, I. et al. CD10 Expression in Epithelial and Stromal Cells of Non-small Cell Lung Carcinoma (NSCLC): A Clinic and Pathologic Correlation. Pathol. Oncol. Res. 18, 153–160 (2012). https://doi.org/10.1007/s12253-011-9421-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12253-011-9421-8

Keywords

Search

Navigation