Skip to main content
SpringerLink
Log in

Bacillus manliponensis sp. nov., a new member of the Bacillus cereus group isolated from foreshore tidal flat sediment

  • Note
  • Published:
The Journal of Microbiology Aims and scope Submit manuscript

Abstract

A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6T, was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6T is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C15:0 and summed feature 3 (containing C16:1 ω7c/iso-C15:0 2OH, and/or iso-C15:0 2OH/C16:1 ω7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6T and B. cereus group was found to be in a range of 22.8–42.3%, and thus BL4-6T represents a unique species. On the basis of these studies, strain BL4-6T (=KCTC 13319T =JCM 15802T) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahmed, I., A. Yokota, A. Yamazoe, and T. Fujiwara. 2007. Proposal of Lysinibacillus boronitolerans gen. nov., sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. Int. J. Syst. Evol. Microbiol. 57, 1117–1125.

    Article  PubMed  CAS  Google Scholar 

  • Antwerpen, M.H., M. Schellhase, E. Ehrentreich-Forster, F. Bier, W. Witte, and U. Nubel. 2007. DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus. Mol. Cell. Probes 21, 152–160.

    Article  PubMed  CAS  Google Scholar 

  • Ash, C., J.A. Farrow, S. Wallbanks, and M.D. Collins. 1991. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett. Appl. Microbiol. 13, 202–206.

    Article  CAS  Google Scholar 

  • Chang, Y.H., J. Han, J. Chun, K.C. Lee, M.S. Rhee, Y.B. Kim, and K.S. Bae. 2002. Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. Int. J. Syst. Evol. Microbiol. 52, 377–381.

    PubMed  CAS  Google Scholar 

  • Chang, Y.H., M.Y. Jung, I.S. Park, and H.M. Oh. 2008. Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil. Int. J. Syst. Evol. Microbiol. 58, 2316–2320.

    Article  PubMed  CAS  Google Scholar 

  • Claus, D. and R.C.W. Berkeley. 1986. Genus Bacillus Cohn 1872, p. 1105–1140. In P.H.A. Sneath, N.S. Mair, M.E. Sharpe, and J.G. Holt (eds.), Bergey’s manual of systematic bacteriology, vol. 2. The Williams and Wilkins Co., Baltimore, USA.

    Google Scholar 

  • Drobniewski, F.A. 1993. Bacillus cereus and related species. Clin. Microbiol. Rev. 6, 324–338.

    PubMed  CAS  Google Scholar 

  • Ezaki, T., Y. Hashimoto, and E. Yabuuchi. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224–229.

    Article  Google Scholar 

  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 40, 783–791.

    Article  Google Scholar 

  • Felsenstein, J. 1993. PHYLIP (phylogeny inference package), version 3.5c, Seattle: Department of Genetics, University of Washington, USA.

    Google Scholar 

  • Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.

    Article  Google Scholar 

  • Gonzalez, J.M. and C. Saiz-Jimenez. 2002. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4, 770–773.

    Article  PubMed  CAS  Google Scholar 

  • Helgason, E., O.A. Okstad, D.A. Caugant, H.A. Johansen, A. Fouet, M. Mock, I. Hegna, and A.B. Kolsto. 2000. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis — One species on the basis of genetic evidence. Appl. Environ. Microbiol. 66, 2627–2630.

    Article  PubMed  CAS  Google Scholar 

  • Henderson, I., C.J. Duggleby, and P.C.B. Turnbull. 1994. Differentiation of Bacillus antliracis from other Bacillus cereus group bacteria with the PCR. Int. J. Syst. Bacteriol. 44, 99–105.

    Article  PubMed  CAS  Google Scholar 

  • Jung, M.Y., J.S. Kim, and Y.H. Chang. 2009. Bacillus acidiproducens sp. nov., vineyard soil isolates that produce lactic acid. Int. J. Syst. Evol. Microbiol. 59, 2226–2231.

    Article  PubMed  CAS  Google Scholar 

  • Jung, M.Y., W.K. Paek, I.S. Park, J.R. Han, Y. Sin, J. Paek, M.S. Rhee, H. Kim, H.S. Song and Y.H. Chang. 2010a. Bacillus gaemokensis sp. nov., isolated from foreshore tidal flat sediment from the Yellow Sea. J. Microbiol. 48, 867–871.

    Article  PubMed  Google Scholar 

  • Jung, M.Y., W.K. Paek, I. Styrak, and Y.H. Chang. 2010b. Proposal of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus mas siliensis and Bacillus odysseyi to Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended descriptions of the genus. Int. J. Syst. Evol. Microbiol. 60, 3003.

    Article  PubMed  CAS  Google Scholar 

  • Kämpfer, P., K. Blasczyk, and G. Auling. 1994. Characterization of Aeromonas genomic species by using quinone, polyamine, and fatty acid patterns. Can. J. Microbiol. 40, 844–850.

    Article  PubMed  Google Scholar 

  • Kaneko, T., R. Nozaki, and K. Aizawa. 1978. Deoxyribonucleic acid relatedness between Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. Microbiol. Immunol. 22, 639–641.

    PubMed  CAS  Google Scholar 

  • Kim, W., J.Y. Kim, S.L. Cho, S.W. Nam, J.W. Shin, Y.S. Kim, and H.S. Shin. 2008. Glycosyltransferase — a specific marker for the discrimination of Bacillus anthracis from the Bacillus cereus group. J. Med. Microbiol. 57, 279–286.

    Article  PubMed  CAS  Google Scholar 

  • Ko, K.S., J.M. Kim, J.W. Kim, B.Y. Jung, W. Kim, I.J. Kim, and Y.H. Kook. 2003. Identification of Bacillus anthracis by rpoB sequence analysis and multiplex PCR. J. Clin. Microbiol. 41, 2908–2914.

    Article  PubMed  CAS  Google Scholar 

  • Komagata, K. and K. Suzuki. 1987. Lipid and cell-wall analysis in bacterial systematics. In R.R. Colwell and R. Grigorova (eds.), Method in microbiology, vol. 19, pp. 161–207. Academic press, London, UK.

    Google Scholar 

  • Lechner, S., R. Mayr, K.P. Francis, B.M. Pruss, T. Kaplan, E. Wiessner-Gunkel, G.S. Stewart, and S. Scherer. 1998. Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. Int. J. Syst. Bacteriol. 48, 1373–1382.

    Article  PubMed  CAS  Google Scholar 

  • Nakamura, L.K. and M.A. Jackson. 1995. Clarification of the taxonomy of Bacillus mycoides. J. Appl. Microbiol. 45, 46–49.

    CAS  Google Scholar 

  • Nakamura, L.K. 1998. Bacillus pseudomycoides sp. nov. Int. J. Syst. Bacteriol. 48, 1031–1035.

    Article  PubMed  CAS  Google Scholar 

  • Priest, F.G., M. Goodfellow, and C. Todd. 1988. A numerical classification of the genus Bacillus. J. Gen. Microbiol. 134, 1847–1882.

    PubMed  CAS  Google Scholar 

  • Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    PubMed  CAS  Google Scholar 

  • Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI, Newark, DE, USA.

    Google Scholar 

  • Schleifer, K.H. 1985. Analysis of the chemical composition and primary structure of murein. Methods Microbiol. 18, 123–156.

    Article  CAS  Google Scholar 

  • Smibert, R.M. and N.R. Krieg. 1994. Phenotypic characterization. In P. Gerhardt, R.G.E. Murray, W.A. Wood, and N.R. Krieg, Methods for General and Molecular Bacteriology, p. 607–654. American Society for Microbiology. Washington, DC, USA.

    Google Scholar 

  • Stackebrandt, E. and J. Ebers. 2006. Taxonomic parameters revisited: tarnished gold standards. Microbiol. Today 33, 152–155.

    Google Scholar 

  • Thorne, C.B. 1993. Bacillus anthracis, In A.L. Sonenshein, J.A. Hoch, and R. Losick (eds.), Bacillus subtilis and other gram-positive bacteria, pp. 113–124. American Society for Microbiology, Washington D.C, USA.

    Google Scholar 

  • Vogler, A.J., J.D. Busch, S. Percy-Fine, C. Tipton-Hunton, K.L. Smith, and P. Keim. 2002. Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus. Antimicrob. Agents Chemother. 46, 511–513.

    Article  PubMed  CAS  Google Scholar 

  • Wayne, L.G., D.J. Brenner, R.R. Colwell, P.A.D. Grimont, O. Kandler, M.I. Krichevsky, L.H. Moore, W.E.C. Moore, R.G.E. Murray, E. Stackebrandt, and et al. 1987. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon, 305-806, Republic of Korea

    Min Young Jung, Jeongheui Lim & Young-Hyo Chang

  2. Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeonbuk, 580-185, Republic of Korea

    Joong-Su Kim

  3. National Science Museum, Daejeon, 305-705, Republic of Korea

    Woon Kee Paek

  4. Korea Institute of Environmental Ecology, Daejeon, 305-301, Republic of Korea

    Hansoo Lee

  5. School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-744, Republic of Korea

    Pyoung Il Kim

  6. Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine, Daejeon, 305-811, Republic of Korea

    Jin Yeul Ma

  7. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, 156-756, Republic of Korea

    Min Young Jung & Wonyong Kim

Authors
  1. Min Young Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joong-Su Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Woon Kee Paek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jeongheui Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hansoo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pyoung Il Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jin Yeul Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wonyong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Young-Hyo Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Young-Hyo Chang.

Additional information

Supplemental material for this article may be found at http://www.springer.com/content/120956

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jung, M.Y., Kim, JS., Paek, W.K. et al. Bacillus manliponensis sp. nov., a new member of the Bacillus cereus group isolated from foreshore tidal flat sediment. J Microbiol. 49, 1027–1032 (2011). https://doi.org/10.1007/s12275-011-1049-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-011-1049-6

Keywords

Search

Navigation