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Over-expression of dehydrin gene, OsDhn1, improves drought and salt stress tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.)

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Abstract

Various environmental stresses limit the plant growth and productivity. Earlier we reported the stress inducible dehydrin gene, OsDhn1, in rice. In this study we generated OsDhn1-overexpression transgenic rice plants (OsDhn1-OXs) to understand the dehydrin function. OsDhn1-OXs showed an enhanced drought and salt stress tolerance as judged by chlorophyll fluorescence (Fv/Fm), fresh and dry weight, water and chlorophyll content, and survival ratio. Furthermore, OsDhn1-OXs showed significantly increased tolerance to methyl viologen (MV)-induced oxidative stress. Under salt and drought stress condition, OsDhn1-OXs maintained relatively low level of H2O2 as compared to wild type plants. Taken together, it is suggested that OsDhn1 plays an important role in the stress tolerance via scavenging reactive oxygen species (ROS).

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Authors and Affiliations

  1. Department of Life Science, Sogang University, Seoul, 121-742, Korea

    Manu Kumar, Sang-Choon Lee, Ji-Youn Kim, Soo-Jin Kim & Seong-Ryong Kim

  2. Department of Botany, University of Yangon, 11041, Yangon, Myanmar

    San San Aye

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  1. Manu Kumar
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  2. Sang-Choon Lee
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  3. Ji-Youn Kim
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  4. Soo-Jin Kim
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  6. Seong-Ryong Kim
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Correspondence to Seong-Ryong Kim.

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Kumar, M., Lee, SC., Kim, JY. et al. Over-expression of dehydrin gene, OsDhn1, improves drought and salt stress tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.). J. Plant Biol. 57, 383–393 (2014). https://doi.org/10.1007/s12374-014-0487-1

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