Physiological role of glucose-phosphorylating enzymes in Saccharomyces cerevisiae

doi:10.1016/0003-9861(77)90544-6

Archives of Biochemistry and Biophysics

Volume 182, Issue 2, August 1977, Pages 639-645

https://doi.org/10.1016/0003-9861(77)90544-6 Get rights and content

Abstract

Starting with a mutant of Saccharomyces cerevisiae lacking glucokinase and both the hexokinase isozymes P1 and P2, strains were constructed, by genetic crosses, that carry single glucose-phosphorylating enzymes. The P1 and P2 isozymes and a structurally altered form of P1 hexokinase were partially purified from these strains. Hexokinases P1, P2, and the altered P1 enzyme, respectively, phosphorylate fructose nearly four, two, and ten times as fast as they phosphorylate glucose. Strains bearing P1 show a pronounced Pasteur reaction and phosphorylate glucose, fructose, and mannose faster than those bearing the P2 isozyme. However, there is no appreciable difference between these two hexokinases in regard to the rate and the extent of growth that they sustain. The ability of yeast to grow on a particular sugar is contingent only upon the presence of an enzyme that phosphorylates it. Glucokinase seems to be responsible for catalyzing nearly half of the glucose flux in the wild type yeast. Strains bearing glucokinase alone do show a Pasteur effect.

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Physiological role of glucose-phosphorylating enzymes in Saccharomyces cerevisiae

Abstract

J. Biol. Chem

J. Biol. Chem

Anal. Biochem

Arch. Biochem. Biophys