The inactivation of yeast enolase by 2,3-butanedione
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Production and properties of enzymes that activate and produce carbon monoxide
2018, Methods in EnzymologyIdentification and characterization of oxalate oxidoreductase, a novel thiamine pyrophosphate-dependent 2-oxoacid oxidoreductase that enables anaerobic growth on oxalate
2010, Journal of Biological ChemistryCitation Excerpt :OOR and PFOR proteins were mixed with 0.5% Coomassie G-250 before loading gels. Protein concentrations were determined by the rose bengal method (19), using a lysozyme standard. The concentration of TPP bound to OOR was determined by a fluorescent thiochrome assay (20).
A glycyl free radical as the precursor in the synthesis of carbon monoxide and cyanide by the [FeFe]-hydrogenase maturase HydG
2010, FEBS LettersCitation Excerpt :The number of reconstituted [4Fe–4S] clusters was assessed by UV–visible spectroscopy. The iron content was determined by the method of Fish [22] and the protein concentration was determined by the Rose Bengal method [23]. Because the phosphate ion is known to be a good Fe3+ chelator and HydG activity is dependant on Fe and S content, we adapted the activity assay from Driesener et al. [17].
Evidence that the heme regulatory motifs in heme oxygenase-2 serve as a thiol/disulfide redox switch regulating heme binding
2007, Journal of Biological ChemistryCitation Excerpt :The mass spectroscopic results were analyzed using the MASCOT search engine. Spectroscopic and Analytical Methods—Protein concentration was calculated based on the rose bengal method (30) using a standard curve generated using known amounts of HO-2, the concentration of which was determined by dry weight. The heme binding affinity of each of the HO-2 variants was determined by adding heme to the reference and sample (containing HO-2) cuvettes and measuring the difference spectrum from 350 to 750 nm in an Olis updated Cary 14 double-beam spectrophotometer.
Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer: Role of an active site asparagine residue in activation of methyl transfer by methyltransferases
2007, Journal of Biological ChemistryCitation Excerpt :MeTr was then purified from the cell-free extract using a 150-ml phenyl-Sepharose column and buffer-exchanged and concentrated by ultrafiltration into 50 mm Tris, pH 7.6, containing 0.1 m NaCl and 2 mm DTT. The concentration of protein was determined with the rose bengal method (16). A typical yield from one liter of culture was 50 mg of MeTr.
Regulation of anaerobic dehalorespiration by the transcriptional activator CprK
2004, Journal of Biological Chemistry