Effects of adenosine on liver cell damage induced by carbon tetrachloride

doi:10.1016/0006-2952(84)90631-2

Biochemical Pharmacology

Volume 33, Issue 16, 15 August 1984, Pages 2599-2604

https://doi.org/10.1016/0006-2952(84)90631-2 Get rights and content

Abstract

Adenosine administration delayed the fatty liver and cell necrosis induced by carbon tetrachloride without affecting the action of the hepatotoxin on protein synthesis and liver triacylglycerol release. Adenosine produced a drastic antilipolytic effect accompanied by a decrease in the incorporation of [1-¹⁴C]palmitic acid into triacylglycerols and free fatty acids of the liver. Furthermore, a decrease in the serum levels of ketone bodies was observed at early times. The nucleoside also avoided the release of intracellular enzymes and prevented the lipid peroxidation produced by carbon tetrachloride during the 4 hr of treatment. The protective action of adenosine was transient, lasting 3–4 hr, probably the time required to be metabolized. The results suggest that the antilipolytic effect of the nucleoside, the inhibition of hepatic fatty acid metabolism, and the decrease in carbon tetrachloride-induced lipoperoxidation that it produced are involved in the delayed acute hepatotoxicity induced by carbon tetrachloride.

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MAO activity was expressed as μmol of 4-HOQ formed/1 h incubation per g of tissue wet weight. The amount of aldehydic products generated by lipoperoxidation was determined by the thiobarbituric acid reactive substances (TBARS) (Buege and Aust, 1978) in 10 brain ST and SN per group, modified as previously reported (Hernandez-Munoz et al., 1984). Briefly, homogenate preparation for the MAO-B determination, was taken 1 mg of protein per sample, was diluted in Tris–HCl (150 mM, pH 7.4) buffer.
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Effects of adenosine on liver cell damage induced by carbon tetrachloride

Abstract

Archs Biochem. Biophys.

Biochem. Pharmac.

Biochem. Pharmac.

J. Lipid. Res.

J. Lipid Res.

J. biol. Chem.

J. biol. Chem.

Biochem. Pharmac.

J. Lipid Res.

J. biol. Chem.

Archs Biochem. Biophys.

Biochem. Pharmac.

Biochem. biophys. Res. Commun.

J. biol. Chem.

J. biol. Chem.

Biochem. Pharmac.

Archs. environ. Hlth