Synthesis of RNA and protein during mitosis in mammalian tissue culture cells

doi:10.1016/0014-4827(62)90176-3

Experimental Cell Research

Volume 26, Issue 2, March 1962, Pages 260-268

https://doi.org/10.1016/0014-4827(62)90176-3 Get rights and content

Abstract

Chinese hamster and HeLa tissue culture cells were incubated in ³H uridine or ³H histidine for a few minutes to 1 hr. ³H-uridine incorporation stopped completely during mitosis and ³H-histidine incorporation was severely depressed. RNA synthesis ceased before nucleolar disappearance and before nuclear membrane disintegration. At the time of nuclear membrane breakdown the nucleolus disappeared and a large amount of nuclear RNA was released to the cytoplasm. Cells arrested in metaphase with colchicine for several hours did not synthesize RNA. The capacity of these cells to incorporate ³H histidine appeared to decline as the duration of metaphase arrest increased.

During interphase ³H uridine was incorporated only into nuclear RNA for the first 20 min. Radioactive RNA began to appear in the cytoplasm 10 min later. The transfer of RNA from nucleus to cytoplasm was faster for the more rapidly growing hamster cells than for HeLa.

The nucleolus of hamster cells incorporated very little ³H histidine into protein.

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Synthesis of RNA and protein during mitosis in mammalian tissue culture cells

Abstract

Exptl. Cell Research

Biochim. et Biophys. Acta

Biochim. et Biophys. Acta

Biochim. et Biophys. Acta

Exptl. Cell Research

Biochim. et Biophys. Acta

Exptl. Cell Research

Exptl. Cell Research

Exptl. Cell Research

Science

Arch. Biol. (Liège)

J. Biophys. Biochem. Cytol

J. Biophys. Biochem. Cytol