Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyltransferase

https://doi.org/10.1016/0014-4827(82)90412-8Get rights and content

Abstract

Secreted galactosyltransferase from bovine milk was used to induce antibodies cross-reacting with corresponding intracellular enzymes in a variety of cell lines and tissues. In contrast to the original antigen, the reactive intracellular galactosyltransferase appears as individual species (apparent MW approx. 42 000–46 000) in SDS-polyacrylamide gel electrophoresis. In indirect immunofluorescence microscopy affinity-purified IgGs locate the galactosyltransferase in a distinct perinuclear and juxtanuclear position indicative for the Golgi region. The rearrangement of labelled structures upon colcemid or monensin treatment—drugs known to influence Golgi morphology and function—is further proof for a Golgi association. The fate and distribution of Golgi elements during mitosis is described at the light microscopical level using galactosyltransferase as easily identifiable marker. In addition we evaluate the utilization of wheat germ agglutinin (WGA) binding for Golgi identification on tissue culture cells and show that WGA is not a reliable marker for certain cell types such as MDCK.

References (36)

  • DF Steiner et al.

    Ann NY acad sci

    (1980)
  • WW Franke et al.

    Exp cell res

    (1978)
  • G Bruder et al.

    Exp cell res

    (1978)
  • R Barker et al.

    J biol chem

    (1972)
  • K Weber et al.

    Exp cell res

    (1975)
  • B Fleischer et al.

    J biol chem

    (1978)
  • WL Adair et al.

    J biol chem

    (1974)
  • S Moskalewski et al.

    J ultrastr res

    (1976)
  • S Moskalewski et al.

    Exp cell res

    (1975)
  • H Schachter et al.

    J biol chem

    (1970)
  • GE Palade

    Science

    (1975)
  • AM Tartakoff

    Int rev exp pathol

    (1980)
  • V Herzog
  • JE Rothman

    Science

    (1981)
  • M Weinstock et al.

    J cell biol

    (1973)
  • RW Young

    J cell biol

    (1973)
  • MFG Schmidt et al.
  • CP Leblond et al.
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