Enzyme activities in granulation tissue: Energy for collagen synthesis

doi:10.1016/0022-4804(76)90108-6

Journal of Surgical Research

Volume 20, Issue 2, February 1976, Pages 121-125

https://doi.org/10.1016/0022-4804(76)90108-6 Get rights and content

Abstract

A microchemical technique was used to measure the activities of twelve key enzymes of glucose catabolism in granulation tissue of wounds healing by second intention. The glycolytic enzymes exhibited a prominent increase during the first eight days of wound healing, with phosphofructokinase increasing to twelve times normal. The enzymatic reactions of the pentose phosphate shunt and Krebs cycle were not increased as markedly as those of glycolysis. Glutamate dehydrogenase and β-hydroxybutyryl CoA dehydrogenase also exhibited significant increases during wound healing.

References (18)

R.A. Carlsen et al.
Glucosaminoglycan formation around the linear wound
J. Invest. Dermatol.
(1973)
A. Delaunay et al.
Mucopolysaccharides, collagen, and nonfibrillar proteins in inflammation
Internatl. Rev. Conn. Tissue Res.
(1964)
T.K. Hunt et al.
Respiratory gas tensions and pH in healing wounds
Amer. J. Surg.
(1967)
J.J. Hutton et al.
Cofactor and substrate requirement of collagen proline hydroxylase
Arch. Biochem. Biophys.
(1967)
M.J.C. Im et al.
Enzyme activities in regeneration epithelium during wound healing. IV. aminotransferases and NADP-dependent enzymes
J. Surg. Res.
(1973)
P. Santoianni et al.
Fluorometric ultramicroanalysis of deoxyribonucleic acid in human skin
J. Invest. Dermatol.
(1965)
K. Adachi
Enzyme activities in mammalian pigment cells
Adv. Biol. Skin
(1967)
J.P. Bentley
Rate of chondroitin sulfate formation in wound healing
Ann. Surg.
(1967)
J.P. Comstock et al.
Effect of lactate on collagen proline hydroxylase activity in cultured L-929 fibroblasts

There are more references available in the full text version of this article.

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To determine the effect of thiamine (vitamin B₁) on collagen production during wound healing, hydroxyproline content (HP), lysyl oxidase activity (LO), and pepsin-solubilized collagen components were examined in dermis, wound fluid, and artificially induced granulation tissue from surgically wounded rats. Rats were divided into three groups: thiamine-deficient diet (−B₁); thiamine-deficient diet supplemented with 3 mg thiamine-HCl (+3B₁); and thiamine-deficient diet supplemented with 1 mg thiamine-HCl (+B₁) (pair fed). When rats from the −B₁ group were demonstrated to be deficient in urinary thiamine, all of the experimental animals had one polyvinyl alcohol wound chamber implanted subcutaneously. Differences were observed in: HP concentration between −B₁ and both +3B₁ and +B₁ in granulation tissue and wound fluid, and LO between −B₁ and +B₁ in skin and granulation tissue. Both high- and low-molecular-weight collagenous components extracted from granulation tissue were greater for +3B₁ and +B₁, than for −B₁. The most dramatic findings were in the higher molecular weight component characterized as Type III collagen. The alterations observed in the collagen contents and maturation of granulation tissue demonstrate an involvement of thiamine in wound repair and scar development.
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^∗: Supported by NIH Postdoctoral Fellowship 1 F22 GM00928-01 from the National Institute of General Medical Sciences.

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Enzyme activities in granulation tissue: Energy for collagen synthesis

Abstract

J. Invest. Dermatol.

Internatl. Rev. Conn. Tissue Res.

Amer. J. Surg.

Arch. Biochem. Biophys.

J. Surg. Res.

J. Invest. Dermatol.

Enzyme activities in mammalian pigment cells

Adv. Biol. Skin

Rate of chondroitin sulfate formation in wound healing

Ann. Surg.

Effect of lactate on collagen proline hydroxylase activity in cultured L-929 fibroblasts