Elsevier

Virology

Volume 161, Issue 2, December 1987, Pages 305-314
Virology

A novel in vitro DNA packaging system demonstrating a direct role for the bacteriophage λ FI gene product

https://doi.org/10.1016/0042-6822(87)90122-XGet rights and content

Abstract

A new in vitro bacteriophage λ DNA packaging system is described in which all the proteins necessary for head morphogenesis are supplied by extracts of plasmid-transformed cells. This assay is used to demonstrate that the λ FI gene product (gpFl) is necessary for maximal packaging efficiency when proheads and terminase are present in limiting amounts. A 100- to 200-fold decrease in packaging is seen when gpFI is omitted. gpFI is shown to act at and/or after the stage in packaging where proheads bind to the DNA:terminase complex.

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