[2] Techniques in coupled enzyme assays
References (16)
- et al.
FEBS Lett.
(1970) Biochim. Biophys. Acta
(1973)- et al.
J. Theor. Biol.
(1974) - et al.
Anal. Biochem.
(1974) - et al.
Anal. Biochem.
(1974) J. Biol. Chem.
(1970)Biochem. Z.
(1953)An Introduction to the Study of Enzymes
Cited by (72)
Biosensor based on coupled enzyme reactions for determination of arginase activity
2022, BioelectrochemistryAnalytical description of the lag phase in coupled-enzyme substrate assays
2020, Biochemical Engineering JournalMitochondrial glycerol phosphate oxidation is modulated by adenylates through allosteric regulation of cytochrome c oxidase activity in mosquito flight muscle
2019, Insect Biochemistry and Molecular BiologyCitation Excerpt :The contribution of the adenine nucleotide translocator (ANT) activity on ADP regulatory effects on respiration was determined by adding 50 μM carboxyatractyloside (CAT) before ADP titration. Regulation of respiratory rates by ATP was assessed in mechanically permeabilized flight muscle in the absence of ADP, by performing stepwise titration of ATP from 0 to 10 mM in the presence of an ATP-regenerating system (ARS) consisting of 5 mM phosphenolpyruvate, 5 mM MgSO4, 20 U/mL pyruvate kinase, 30 U/mL lactate dehydrogenase and 0.2 mM NADH (Rudolph et al., 1979). In order to evaluate the role of the arginine kinase (ArgK) in the regulation of flight muscle respiration, a single insect thorax was added into the O2K chamber filled with 2 mL of respiration buffer.
The heme-sensitive regulator SbnI has a bifunctional role in staphyloferrin B production by Staphylococcus aureus
2019, Journal of Biological ChemistryPhase-plane geometries in coupled enzyme assays
2018, Mathematical BiosciencesCitation Excerpt :The observable enzyme catalyzed reaction is known as the indicator or monitor reaction [2]. The non-observable enzyme catalyzed reaction can be of varying complexity; it may contain linear inhibition or exhibit enzyme degradation [3]. Regardless of the nature of the non-observable reaction, there are two general mechanisms employed to couple enzyme reactions: the coupled auxiliary enzyme reaction mechanism [4], and zymogen activation coupled to its enzyme catalyzed reaction [5].
A theory of reactant-stationary kinetics for a mechanism of zymogen activation
2018, Biophysical ChemistryCitation Excerpt :In this case, the secondary reaction step (2) is known as the indicator reaction. Traditionally, coupled enzyme assays are designed so that the product of the non-observable reaction is a substrate for the secondary enzyme in the indicator reaction (see [6] for specific applications). While this type of assay is well-studied [7–10], in vitro assays that consist of a zymogen activation step have not been analyzed with the same degree of interest.