Elsevier

Methods in Enzymology

Volume 207, 1992, Pages 149-155
Methods in Enzymology

[8] Prevention of rundown in electrophysiological recording

https://doi.org/10.1016/0076-6879(92)07010-LGet rights and content

Publisher Summary

This chapter discusses and evaluates several methods that reduce or avoid the problems presented by whole-cell recording, and are used to prevent rundown in electrophysiological experiments. Cell-attached patch recording usually implies that cytoplasm is maintained on the intracellular surface of the plasma membrane. With this configuration tingle-channel currents are usually observed. However, if channel density is very high or if very large pipettes are used, macroscopic currents may be observed in cell-attached patches. Such currents are modulable by second messengers and do not wash out. These techniques preserve—to varying extents—the integrity of the cytoplasm. Each has its own virtues and pitfalls, and it is unlikely that one method will suffice for all purposes. Usually, the choice of technique involves a compromise between the ability to determine the composition of the intracellular solution and the preservation of the cytoplasm.

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