Cell
Volume 15, Issue 3, November 1978, Pages 945-954
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Article
Studies on histone organization in the nucleosome using formaldehyde as a reversible cross-linking agent

https://doi.org/10.1016/0092-8674(78)90278-7Get rights and content

Abstract

A new procedure is described which allows selective reversal of formaldehyde cross-linking in both histone-histone and histone-DNA of nuclei isolated from calf thymus. All ten possible dimers of the four non-H1 histones, H3, H2B, H2A and H4, are observed, the major dimers being H3-H3, H3-H2A, H2B-H2A, H2a-H2A and two separate dimers of H2B-H4. Although oligomers of the non-H1 histones are formed by prolonged treatment with this reagent, 50% of the histones continue to remain resistant to cross-linking with each other. For those histones which cross-link, the site of cross-linking within the molecules is located in the “core” (trypsin-resistant) region and therefore indicates proximities for these molecules within the nucleosome. The core region also cross-links to DNA, indicating intimate interactions between this region in all the non-H1 histones with DNA.

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    Present address: Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242.

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