Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis - ScienceDirect

Cell

Volume 41, Issue 1, May 1985, Pages 177-190

https://doi.org/10.1016/0092-8674(85)90072-8 Get rights and content

Abstract

The cell type-specific expression of the major nuclear lamina polypeptides (“lamins”) during development of Xenopus was studied using two monoclonal antibodies (L₀46F7: specific for L_III, the single lamin of oocytes; PKB8: specific for L_I and L_II of some somatic cells). In the oocyte, L_III localizes in the nuclear polymer, but upon nuclear envelope breakdown it is solubilized to a form sedimenting at 9 S. In early embryos, L_III contributes to nuclear lamina formation until its depletion. Correspondingly, L_I and L_III begin to be expressed at a specific point in embryogenesis and appear to be integrated with L_III into a common lamina structure. Later in development, L_III reappears as a prominent nuclear lamina protein but only in certain cells (neurons, muscle cells, and diplotene oocytes). We conclude that amphibian lamins represent a family of proteins expressed in relation to certain programs of cell differentiation.

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