Cell
ArticleDifferential regulation of Ultrabithorax in two germ layers of drosophila
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2011, Mechanisms of DevelopmentCitation Excerpt :Taken together, these results indicate that N receptor signaling promotes the expression of the joint-specific RhoGAPs and RhoGEFs at presumptive joints either directly or indirectly. To identify novel regulators of epithelial morphogenesis and specifically those affecting apical constriction and epithelial invagination of presumptive joints, we depleted the function of individual RhoGEFs and RhoGAPs encoded in Drosophila by inducible hairpin RNA-mediated interference (RNAi) in the distal part of developing leg imaginal discs and assayed for phenotypes in adult legs (Bienz et al., 1988; Dietzl et al., 2007). In control experiments, we found that expression of hairpin RNAs to deplete a green fluorescent protein (GFP) caused no phenotypes (not shown).
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