Cell
Volume 65, Issue 7, 28 June 1991, Pages 1213-1224
Journal home page for Cell

Article
Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1

https://doi.org/10.1016/0092-8674(91)90016-RGet rights and content

Abstract

Cells of the yeast S. cerevisiae choose bud sites in an axial or bipolar spatial pattern depending on their cell type. We have identified a gene, BUD5, that resembles BUD1 and BUD2 in being required for both patterns; bud5 mutants also exhibit random budding in all cell types. The BUD5 nucleotide sequence predicts a protein of 538 amino acids that has similarity to the S. cerevisiae CDC25 product, an activator of RAS proteins that catalyzes GDP-GTP exchange. Two potential targets of BUD5 are known: BUD1 (RSR1) and CDC42, proteins involved in bud site selection and bud formation, respectively, that have extensive similarity to RAS. We also show that BUD5 interacts functionally with a gene, BEM1, that is required for bud formation. This interaction provides further support for the view that products involved in bud site selection guide the positioning of a complex necessary for bud formation.

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      Citation Excerpt :

      These budding patterns are enabled by three groups of bud genes. The first group (bud3, bud4, axl1 and axl2/bud10) is specifically required for the axial budding pattern [68–71], the second group (bud7, bud8, bud9, rax1 and rax2) is required for bipolar budding [72–74], whilst the third group (rsr1/bud1, bud2 and bud5) is necessary for both budding patterns [75,76]. Bud3p and Bud4p localise to the bud neck from G2 and are thought to recruit Axl1p and Axl2p [77,78].

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    Present address: Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048.

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