Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression

doi:10.1016/0147-619X(85)90068-X

Plasmid

Volume 13, Issue 2, March 1985, Pages 149-153

https://doi.org/10.1016/0147-619X(85)90068-X Get rights and content

Abstract

Derivatives of plasmid pRK290 that are useful for cloning and for analyzing gene expression in a wide variety of Gram-negative bacteria are described. A smaller broad host range plasmid derived from RK2, with properties similar to that of pRK290, is also described.

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Citation Excerpt :
The structures of the plasmids are drawn in Fig. 1. We adopted vectors pACYC184 (Rose, 1988), pRK404 (Ditta et al., 1985), and pUC18 (pUC19) (Takara Bio, Ohtsu, Japan) and other Duet-1 vectors (Merck, MO, USA) as the backbones for the carotenoid plasmids (Fig. 1A–D). Carotenoid biosynthesis genes carried by these vectors are listed in Tables 1 and 2.
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Short communicationPlasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression

Abstract

J. Mol. Biol

Gene

Gene

J. Biol. Chem

Gene

Nucleic Acids Res

Short communication
Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression