Research paperValidation of a neocortical slice preparation for the study of epileptiform activity
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Cited by (42)
Tissue oxygen partial pressure as a viability metric for ex vivo brain tissue slices
2023, Journal of Neuroscience MethodsMicroalgae-based photosynthetic strategy for oxygenating avascularised mouse brain tissue – An in vitro proof of concept study
2021, Brain ResearchCitation Excerpt :Following tissue preparation, the slices were transferred to a perfusion recording bath and maintained under submerged conditions in no-Mg aCSF. Exposure of cortical slices to no-Mg aCSF activates the tissue by unblocking NMDA receptors (Aram and Lodge, 1988), resulting in the generation of repeating, spontaneous paroxysmal events known as seizure-like events (SLEs). SLEs are recordable from all cortical layers and regions and reflect the coordinated firing of mixed populations of excitatory and inhibitory neurons.
Cerebrospinal fluid oxygen optimisation for rescue of metabolically challenged in vitro cortical brain tissue
2020, IBRO ReportsCitation Excerpt :Subsequently, the slices were immersed in no-Mg aCSF at room temperature (approximately 21 °C) for at least 60 min. Exposure to no-Mg aCSF activates the tissue by unblocking NMDA receptors (Aram and Lodge, 1988), resulting in the generation of repeating, spontaneous paroxysmal events commonly known as seizure-like events (SLEs). SLE activity is sensitive to hypoxic challenge (Voss et al., 2020) and provides a relatively non-invasive electrophysiological method for quantifying tissue viability over time (Voss et al., 2013).
Impact of variation in tissue preparation methodology on the functional outcome of neocortical mouse brain slices
2020, Brain ResearchCitation Excerpt :Subsequently the slices were immersed in no-Mg aCSF at room temperature (approximately 21 °C) for at least 60 min. Exposure to no-Mg aCSF activates the tissue by unblocking NMDA receptors (Aram and Lodge, 1988), resulting in the generation of repeating, spontaneous paroxysmal events commonly known as seizure-like events (SLEs). The slices were transferred one at a time to a submersion-style perfusion bath (Kerr Scientific Instruments, Dunedin, NZ) which was continuously flowed with the aCSF solution of choice, at an adjustable flow rate.
Relationship between artificial cerebrospinal fluid oxygenation, slice depth and tissue performance in submerged brain slice experiments
2020, Neuroscience LettersCitation Excerpt :Subsequently the slices were immersed in aCSF void of magnesium (no-Mg aCSF) at room temperature (approximately 21 °C) for at least 60 minutes. Exposure to no-Mg aCSF activates the tissue by unblocking NMDA receptors [6], resulting in the generation of repeating, spontaneous paroxysmal events known as seizure-like events (SLEs) [7]. Normal aCSF was composed of 125 mM NaCl, 2.5 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 1.25 mM NaH2PO4, 2.5 mM NaHCO3, 10 mM HEPES and 10 mM D-glucose.
Sustained down-regulation of β-dystroglycan and associated dysfunctions of astrocytic endfeet in epileptic cerebral cortex
2014, Journal of Biological ChemistryCitation Excerpt :In sharp contrast, the dynamics of the biochemical components in endfeet under (patho)physiological conditions are poorly understood. In this study, we utilized the well characterized zero magnesium (0 Mg2+) brain slice model of epilepsy, which induces epileptiform discharges in wide areas of the neocortex (15–17), as a model system to examine the effect of (patho)physiological neuronal activities on the biochemical organizations of endfeet in cortical astrocytes. Here, we report that upon the epileptic activation of neurons, βDG is down-regulated with distinct kinetics from the pathological activation of neurons and with a concurrent dysfunction of the astrocytic endfeet.