The aerobic energy metabolism of the juvenile Fasciola hepatica
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Insights into Fasciola hepatica Juveniles: Crossing the Fasciolosis Rubicon
2021, Trends in ParasitologyCitation Excerpt :Glucose catabolism is the best described metabolic difference between FhNEJ and the adult worm. Adult flukes are fully anaerobic, and the main product of glucose cytosolic degradation at this stage is acetate, while FhNEJ mainly use the aerobic route when freshly excysted and later switch to a facultative anaerobic profile that keeps specific aerobic paths of glucose breakdown both in the presence and absence of oxygen [27–29]. Therefore, it could be concluded that F. hepatica juveniles are gradually forced to switch from an aerobic metabolism to the anaerobic glycolysis that is fully developed in adult worms, in which the level of oxygen reaching internal tissues by diffusion is lower than in juvenile stages due to their larger size and the essentially anaerobic environment of the bile ducts.
Schistosoma mansoni does not and cannot oxidise fatty acids, but these are used for biosynthetic purposes instead
2019, International Journal for ParasitologyCitation Excerpt :The acidified supernatant was neutralised by the addition of 6 M NaOH. The labelled metabolic end products in the supernatant were analysed by anion exchange chromatography on a Dowex 1X8, 100–200 mesh column (Serva) (60 × 1.1 cm) in chloride form (Tielens et al., 1981). The column was eluted successively with 200 ml of 5 mM HCl and 130 ml of 0.2 M NaCl.
Lipids Are the Preferred Substrate of the Protist Naegleria gruberi, Relative of a Human Brain Pathogen
2018, Cell ReportsCitation Excerpt :In aerobic incubations the gas phase was air. For anaerobic incubations the medium was degassed, flasks were flushed for 5 min with nitrogen and prior to closing the bottles, ascorbate oxidase (100 U) was added to the anaerobic incubations to remove any remaining oxygen (Tielens et al., 1981). To determine the effects of respiratory chain inhibitors on the cell growth of N. gruberi NEG-M, trophozoites were seeded in PYNFH medium at a density of 30,000 trophozoites per ml.
Acetyl:succinate CoA-transferase in procyclic Trypanosoma brucei. Gene identification and role in carbohydrate metabolism
2004, Journal of Biological ChemistryCitation Excerpt :After removal of carbon dioxide, the acidified supernatant was separated from the cells by centrifugation (4 °C, 10 min at 500 × g) and neutralized by the addition of 40 μlof6 m NaOH. Analysis of the labeled end products occurred by anion-exchange chromatography on a Dowex 1X8, 100–200 mesh column (Serva), 60 × 1.1 cm in chloride form (44). The column was eluted successively with 200 ml of 5 mm HCl, 130 ml of 0.2 m NaCl, and 130 ml of 0.5 m NaCl.
Procyclic Trypanosoma brucei do not use Krebs cycle activity for energy generation
2003, Journal of Biological ChemistryCitation Excerpt :The simultaneous presence of KCN and SHAM resulted within 1 h in cell death of both the wild type and aconitase knockout procyclic trypanosomes (not shown). In addition, the complete removal of oxygen from the incubation, (using ascorbate and ascorbate oxidase as was described previously (31)), also resulted in rapid death of the organisms (not shown). Apparently, procyclic T. brucei cells are completely dependent on the use of oxygen by the respiratory chain for their energy generation and survival.
Respiratory Chain of the lung fluke paragonimus westermani - facultative anaerobic mitochondria
1994, Archives of Biochemistry and Biophysics