Regular paperEffects of aphidicolin and hydroxyurea on the cell cycle and differentiation of Trypanosoma brucei bloodstream forms
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Visualizing mesoderm and neural crest cell dynamics during chick head morphogenesis
2020, Developmental BiologyCitation Excerpt :To attempt a change in growth rate in vivo, we microinjected a cocktail of Aphidicolin (1 mg/mL), Hydroxyurea (5mM) and DiD into the most lateral mesoderm that could be reached in HH10 embryos (Fig. 7F). Aphidicolin and hydroxyurea are both well-known to inhibit the cell cycle in S-phase and halt proliferation (Levenson and Hamlin, 1993; Mutomba and Wang, 1996). We microinjected control embryos with 10% DMSO and DiD.
Functional characterization of TcCYC2 cyclin from Trypanosoma cruzi
2012, Experimental ParasitologyCitation Excerpt :In addition, HU induces double strand breaks in cells starting mitosis. It is interesting that T. brucei, rather than being arrested at G1 phase (Mutomba and Wang, 1996), could be induced to synchrony in S phase using low concentrations of HU (Chowdhury et al., 2008; Forsythe et al., 2009), suggesting that different check points operate in both organisms. In eukaryotes, some complexes formed by cyclins-CDKs need to be deactivated if DNA damage occur, in order to stop the progression of the cell cycle until the DNA is repaired (Gillis et al., 2009; Guo et al., 2000; Pontano and Diehl, 2009).
Inactivation of a Macronuclear Intra-S-phase Checkpoint in Tetrahymena thermophila with Caffeine Affects the Integrity of the Micronuclear Genome
2011, ProtistCitation Excerpt :Therefore, we propose that in Tetrahymena low doses of APH induce only partial inhibition of polymerase α and perhaps polymerase δ⋅ The inhibition is strong enough to cause cell division arrest, but it does not prevent macronuclear DNA over-replication, even if this replication is slowed down. This explanation is consistent with the following data (i) increase in DNA content did not occur in cells treated with high concentration of APH, (ii) Luciani et al. (2004) showed that low concentrations of APH induced only partial inhibition of DNA strand elongation in early replication forks, and arrested firing of the late forks in mammalian nuclei, (iii) continuation of DNA replication and induction of polyploidy in the presence of low concentration of APH, and HU occurred in Trypanosoma cruzi (Mutomba and Wang 1996). It is relevant for this discussion that in Tetrahymena mutants of telomerase templates (Kirk et al. 1997, 2008; Petcherskaia et al. 2003; Yu et al. 1990) and in knockout mutants of the Pot-1 gene (Jacob et al. 2007) cell division arrest is correlated with an increase of macronuclear DNA content and cell size.
Giardia intestinalis: Aphidicolin influence on the trophozoite cell cycle
2010, Experimental ParasitologyCell-cycle synchronisation of bloodstream forms of Trypanosoma brucei using Vybrant DyeCycle Violet-based sorting
2010, Molecular and Biochemical ParasitologyHydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei
2009, Molecular and Biochemical Parasitology