Immunohistochemical evidence of gastro-entero-pancreatic neurohormonal peptides of vertebrate type in the nervous system of the larva of a dipteran insect, the hoverfly, Eristalis aeneus

doi:10.1016/0167-0115(80)90271-2

Regulatory Peptides

Volume 1, Issue 3, December 1980, Pages 187-204

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Abstract

Using rabbit and guinea-pig antisera, raised against GEP neurohormonal peptides of mammalian origin, cells were observed in the brain and/or in the fused ventral ganglia of the last (fifth) larval instar of the hoverfly, Eristalis aeneus, being immunoreactive with antisera against insulin, somatostatin, glucagon, PP, secretin, gastrin/CCK/caerulein; substance P, enkephalin and endorphin. Most of these GEP neurohormonal peptides also occurred in nerve fibers. No immunoreactive cells or nerve fibers could be detected with antisera against GIP, VIP, (the central fragments of) CCK, bombesin or neurotensin. The antisera tested failed to reveal any immunoreactive cells or nerves in Weusmann's ring (fused corpus allatum/corpus cardiacum and thoracic gland) or in different parts of the alimentary tract.

The observations support the hypothesis that neuronal GEP hormonal peptide production in the brain is a genuinely original mechanism and the appearance of endocrine cells in the gut a later feature in evolution.

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Cited by (0)

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Regulatory Peptides

Abstract

References (36)

Immunochemical evidence for a gastrin-like peptide in insect neuroendocrine system

Gen. Comp. Endocr.

Insulin-like and glucagon-like peptides in insect hemolymph

Insect Biochem.

Immunochemical studies on cholecystokinin. II. Distribution and molecular heterogeneity in the central nervous system and small intestine of man and hog

J. Biol. Chem.

Vertebrate neuropeptide-like substances in the sub-oesophageal ganglion of two insects: Locusta migratoria R. and F. (Orthoptera) and Bombyx mori L. (Lepidoptera): immunocytochemical investigation

Gen. Comp. Endocr.

Neuronal localization of pancreatic polypeptide (PP) and vasoactive intestinal peptide (VIP) immunoreactivity in the earthworm (Lumbricus terrestris)

Brain Res. Bull.

Gut hormone overview

Glucagon-like polypeptides in canine brain

Diabetes

Sur l'existence dans la pars intercéreb alis d'un insecte (Locusta migratoria R. et F.) de cellules neurosécrétrices fixant un antisérum antisomatostatin

C.R. Acad. Sci.

Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphore vomitoria (Diptera)

cell Tiss. Res.

The isolation of material displaying insulin-like immunological and biological activity from the brain of the blowfly, Calliphora vomitoria

Biochem. J.

A modification of the unnlabeled antibody enzyme method using heterologous antisera for the light microscopic and ultrastructural localization of insulin, glucagon, and growth hormone

J. Histochem. Cytochem.

Phylogeny of the GEP neuroendocrine system. A review with special reference to the occurrence of CCK-like and neurotensin-like polypeptides in lower vertebrates and invertebrates

Some phylogenetic aspects on the enteroinsular axis with particular regard to the appearance of the gastric inhibitory polypeptide

Front. Horm. Res.

Neurosecretion

A silver nitrate strain for α₂-cells in human pancreatic islets

Acta Soc. Med. Ups.

Identification of insulin in rat brain

Insulin-like and glucagon-like hormones in insects

The organization of insect nervous system

Cited by (0)

Immunohistochemical evidence of gastro-entero-pancreatic neurohormonal peptides of vertebrate type in the nervous system of the larva of a dipteran insect, the hoverfly, Eristalis aeneus

Abstract

Gen. Comp. Endocr.

Insect Biochem.

J. Biol. Chem.

Gen. Comp. Endocr.

Brain Res. Bull.

Gut hormone overview

Glucagon-like polypeptides in canine brain

Diabetes

Sur l'existence dans la pars intercéreb alis d'un insecte (Locusta migratoria R. et F.) de cellules neurosécrétrices fixant un antisérum antisomatostatin

C.R. Acad. Sci.

Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphore vomitoria (Diptera)

cell Tiss. Res.

The isolation of material displaying insulin-like immunological and biological activity from the brain of the blowfly, Calliphora vomitoria

Biochem. J.

A modification of the unnlabeled antibody enzyme method using heterologous antisera for the light microscopic and ultrastructural localization of insulin, glucagon, and growth hormone

J. Histochem. Cytochem.

Phylogeny of the GEP neuroendocrine system. A review with special reference to the occurrence of CCK-like and neurotensin-like polypeptides in lower vertebrates and invertebrates

Some phylogenetic aspects on the enteroinsular axis with particular regard to the appearance of the gastric inhibitory polypeptide

Front. Horm. Res.

Neurosecretion

A silver nitrate strain for α2-cells in human pancreatic islets

Acta Soc. Med. Ups.

Identification of insulin in rat brain

Insulin-like and glucagon-like hormones in insects

The organization of insect nervous system

A silver nitrate strain for α₂-cells in human pancreatic islets