Elsevier

Regulatory Peptides

Volume 11, Issue 3, July 1985, Pages 251-262
Regulatory Peptides

The intestinal mucosa preferentially releases somatostatin-28 in pigs

https://doi.org/10.1016/0167-0115(85)90057-6Get rights and content

Abstract

We studied the molecular forms of somatostatin-like immunoreactivity (SLI), newly released from isolated perfused preparations of the porcine antrum, stomach, pancreas and upper small intestine.

Perfusion effluents were concentrated by Sep-Pak C-18 adsorption, eluted with ethanol, dessicated, and subjected to gel filtration with subsequent radioimmunoassays for somatostatin-14 and N-terminal somatostatin-28 immunoreactivity.

All the SLI newly released from the stomach and antrum eluted at the position of somatostatin-14, and such was also the case for more than 95% of the SLI newly released from the pancreas, while 68 ∓ 7% and 75 ∓ 8% of the SLI newly released from the isolated perfused jejunum and ileum, respectively, corresponded to somatostatin-28.

By reverse phase HPLC the identity of these peptides with synhetic somatostatin-14 and -28 was established.

References (37)

  • Y.C. Patel et al.

    Somatostatin in hypothalamus, extrahypothalamic brain and peripheral tissues of the rat

    Endocrinology

    (1978)
  • A.V. Schally et al.

    Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus

  • F. Esch et al.

    Primary structure of ovine hypothalamic somatostatin 28 and somatostatin 25

  • D.F. Trent et al.

    Heterogeneity of somatostatin-like peptide in rat brain, pancreas and gastrointestinal tract

    Endocrinology

    (1981)
  • Y.C. Patel et al.

    Multiple forms oof immunoreactive somatostatin: Comparison of distribution in neural and non-neural tissues and portal plasma in the rat

    Endocrinology

    (1981)
  • J.J. Holst et al.

    Content and gel filtration profiles of glucagon-like and somatostatin-like immunoreactivity in human fundic mucosa

    J. Clin. Endocrinol. Metab.

    (1983)
  • E. Penman et al.

    Distribution and characterization of immunoreactive somatostatin in human gastrointestinal tract

    Regul. Peptides

    (1983)
  • C. Patzel et al.

    Identification of prosomatostatin in pancreatic islets

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