Elsevier

Genomics

Volume 3, Issue 2, August 1988, Pages 100-109
Genomics

A subfamily of alphoid repetitive DNA shared by the nor-bearing human chromosomes 14 and 22

https://doi.org/10.1016/0888-7543(88)90139-5Get rights and content

Abstract

The nucleotide sequence of members of an α-repeat subfamily shared by human chromosomes 14 and 22 is presented. This subfamily is organized into a higher-order repeat unit composed of a tandem repetition of an ordered array of four related but distinct 340-bp repeat dimers. An analogous situation has been described for a related but distinct subfamily shared by chromosomes 13 and 21. These two subfamilies were further shown not to be present on the homologous chimpanzee chromosomes and therefore must have arisen by rearrangement of the human genome after separation of the two species. The sequence homology between the 1321 and the 1422 subfamilies is about 85%. The 1422 subfamily represents the only major alphoid DNA species on these two chromosomes and is not present elsewhere in the human genome. Fluorescent in situ hybridizations show that sequences from the 1321 and 1422 subfamilies can be used as specific markers for their respective chromosomes.

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