Neuron
Volume 12, Issue 3, March 1994, Pages 675-690
Journal home page for Neuron

TAG-1 can mediate homophilic binding, but neurite outgrowth on TAG-1 requires an L1-like molecule and β1 integrins

https://doi.org/10.1016/0896-6273(94)90222-4Get rights and content

Summary

Subsets of axons in the embryonic nervous system transiently express the glycoprotein TAG-1, a member of the subfamily of immunoglobulin (Ig)-like proteins that contain both C2 class Ig and fibronectin type III domains. TAG-1 is attached to the cell surface by a glycosylphosphatidylinositol linkage and is secreted by neurons. In vitro studies have shown that substrate-bound TAG-1 promotes neurite outgrowth. We have examined the nature of axonal receptors that mediate the neurite-outgrowth promoting properties of TAG-1. Although TAG-1 can mediate homophilic binding, neurite out-growth on a substrate of TAG-1 does not depend on the presence of TAG-1 on the axonal surface. Instead, neurite outgrowth on TAG-1 is inhibited by polyclonal antibodies directed against L1 and, independently, by polyclonal and monoclonal antibodies against β1-containing integrins. These results provide evidence that TAG-1 can interact with cell surfaces in both a homophilic and heterophilic manner and suggest that neurite extension on TAG-1 requires the function of both integrins and an L1-like molecule.

References (70)

  • LemmonV. et al.

    L1-mediated axon outgrowth occurs via a homophilic binding mechanism

    Neuron

    (1989)
  • MiuraM. et al.

    Molecular cloning of cDNA encoding the rat neural cell adhesion molecule L1. Two L1 isoforms in the cytoplasmic region are produced by differential splicing

    FEBS Lett.

    (1991)
  • RathjenF.G. et al.

    Glycoproteins that regulate the growth and guidance of vertebrate axons: domains and dynamics of the immunoglobulin/fibronectin type III subfamily

    Sem. Neurosci.

    (1991)
  • TanakaH. et al.

    Molecular cloning and expression of a novel adhesion molecule, SC1

    Neuron

    (1991)
  • TsiotraP.C. et al.

    Isolation of the cDNA and chromosomal localization of the gene (TAX1) encoding the human axonal glycoprotein TAG-1

    Genomics

    (1993)
  • AlbeldaS.M. et al.

    Identification and characterization of cell-substratum adhesion receptors on cultured human endothelial cells

    J. Clin. Invest.

    (1989)
  • BixbyJ.L. et al.

    Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth

    J. Cell Biol.

    (1990)
  • BossyB. et al.

    Characterization of the integrin α8 subunit: a new integrin β1-associated subunit, which is prominently expressed on axons and on cells in contact with basal laminae in chick embryos

    EMBO J.

    (1991)
  • BuckC.A. et al.

    Integrin, a transmembrane glycoprotein complex mediating cell-substratum adhesion

    J. Cell Sci.

    (1987)
  • BuckC.A. et al.

    Integrin (the CSAT antigen): functionality requires oligomeric integrity

    J. Cell Biol.

    (1986)
  • BunchT.A. et al.

    Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells

    Nucl. Acids Res.

    (1988)
  • BurgoonM.P. et al.

    Structure of the chick neuron-glia cell adhesion molecule, Ng-CAM: origin of the polypeptides and relation to the Ig superfamily

    J. Cell Biol.

    (1991)
  • ChangS. et al.

    Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins

    J. Cell Biol.

    (1987)
  • CunninghamB.A. et al.

    Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing

    Science

    (1987)
  • DoddJ. et al.

    Axon guidance and the patterning of neuronal projections in vertebrates

    Science

    (1988)
  • DohertyP. et al.

    Neuronal process outgrowth of human sensory neurons on monolayers of cells transfected with cDNAs for five human N-CAM isoforms

    J. Cell Biol.

    (1989)
  • ElkinsT. et al.

    Drosophila fasciclin I is a novel homophilic adhesion molecule that along with fasciclin III can mediate cell sorting

    J. Cell Biol.

    (1990)
  • FanJ. et al.

    The organization of F-actin and microtubules in growth cones exposed to a brain-derived collapsing factor

    J. Cell Biol.

    (1993)
  • GennariniG. et al.

    The mouse neuronal cell surface glycoprotein F3: a phosphatidylinositol-anchored member of the immunoglobulin superfamily related to chick contactin

    J. Cell Biol.

    (1989)
  • GreveJ.M. et al.

    Monoclonal antibodies which alter the morphology of cultured chick myogenic cells

    J. Cell Biochem.

    (1982)
  • GrumetM. et al.

    Structure of a new nervous system glycoprotein, Nr-CAM, and its relationship to subgroups of neural cell adhesion molecules

    J. Cell Biol.

    (1991)
  • HarlowE. et al.
  • HaslerT.H. et al.

    cDNA cloning, structural features, and eucaryotic expression of human TAG-1/axonin-1

    Eur. J. Biochem.

    (1993)
  • HoffmanS. et al.

    Kinetics of homophilic binding by E and A forms of the neural cell adhesion molecule

    Proc. Natl. Acad. Sci. USA

    (1983)
  • JessellT.M. et al.

    Floor plate-derived signals and the control of neural cell pattern in vertebrates

    Harvey Lect.

    (1992)
  • Cited by (0)

    *

    Present address: Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080.

    Present address: Laboratory of Developmental Neurobiology, National Institute of Medical Research, The Ridgeway, Mill Hill, London, England.

    View full text