Characterization of a G-protein β-subunit gene from the nematode Caenorhabditis elegans*

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The gene encoding the β-subunit of guanine nucleotide binding regulatory proteins (G-proteins) has been cloned from the nematode Caenorhabditis elegans. The predicted 340 amino acid sequence matches the highly conserved amino acid sequences of previously isolated G-protein β-subunits from mammals and Drosophila. The coding region of the C. elegans β-subunit gene, which has been mapped to the C. elegans chromosome II, is interrupted by eight introns. Southern analysis indicates that C. elegans has only one β-subunit gene. A 2·8 kb (1 kb = 103 bases or base-pairs) transcript derived from this gene could be detected.

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    *

    This research was supported by the Netherlands Cancer Foundation (KWF) and the Netherlands Organization for the Advancement of Pure Scientific Research (NWO).

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