Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genes

doi:10.1016/S0092-8674(85)80031-3

Cell

Volume 41, Issue 2, June 1985, Pages 587-595

https://doi.org/10.1016/S0092-8674(85)80031-3 Get rights and content

Summary

The product of the Ion gene in Escherichia coli, protease La, plays an important role in the degradation of abnormal proteins. To determine whether the presence of abnormal proteins stimulates expression of this gene, we examined its transcription using a Ion-IacZ operon fusion. After the cells synthesized large amounts of aberrant polypeptides (e.g. following incorporation of the arginine analog, canavanine, or production of incomplete proteins with puromycin, or induction of translational errors with streptomycin), these cells showed a two to three fold increase in Ion-IacZ expression. Furthermore, synthesis of a single cloned protein, e.g. human tissue plasminogin activator, caused a similar increase in Ion transcription. This induction of Ion by abnormal proteins requires the heat shock regulatory gene htpR and was not seen in htpR⁻ cells. Under these various conditions, other heat shock proteins were also induced. Thus, the appearance of aberrant cell proteins may be a common signal under many adverse conditions for the induction of cell protease (or proteases) and other heat shock proteins.

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Cell

Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genes

Summary

Gene

FEMS Microbiol. Letters

J. Mol. Biol.

Cell

Cell

Cell

Cell

J. Mol. Biol.

Cell

J. Biol. Chem.

J. Biol. Chem.

Biochem. Biophys. Res. Commun.

Cell

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

A gene regulating the heat shock response in Escherichia coli also affects proteolysis

Positive selection for loss of tetracycline resistance

J. Bacteriol.

AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress

Cell

Mutants of Escherichia coli with a defect in the degradation of nonsense fragments

Nature New Biol.

ATPdependent protease activity of the Ion (capR) protein of Escherichia coli K12

The product of the Ion (capR) gene in Escherichia coli is the ATP-dependent protease, protease La

A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12

Molec. Gen. Genet.

Free radical generating systems induce protein degradation and lipid peroxidation by distinct mechanisms

Superoxide radical: an endogenous toxicant

Ann. Rev. Pharmacol. Toxicol.

The heat shock regulatory gene, htpR, influences rates of protein degradation and expression of the Ion gene in Escherichia coli

Studies on the regulation of Ion gene expression in Escherichia coli

Degradation of abnormal proteins in Escherichia coli