Rapid reduction of MDCK cell cholesterol by methyl-β-cyclodextrin alters steady state transepithelial electrical resistance
References (40)
- et al.
Atherosclerosis alters the composition, structure and function of smooth muscle cell plasma membranes
Biochim. Biophys. Acta
(1995) - et al.
Origins of cell polarity
Cell
(1996) - et al.
Hydroxypropylcyclodextrins in parenteral use. I: Lipid dissolution and effects on lipid transfers in vitro
J. Pharm. Sci.
(1992) - et al.
Regulation of tight junction permeability and occludin expression by polyunsaturated fatty acids
Biochem. Biophys. Res. Commun.
(1998) - et al.
Cellular cholesterol efflux mediated by cyclodextrins
J. Biol. Chem.
(1995) - et al.
The role of intracellular cholesterol transport in cholesterol homeostasis
Trends Cell Biol.
(1996) - et al.
Plasma membranes contain half the phospholipid and 90 % of the cholesterol and sphingomyelin in cultured human fibroblasts
J. Biol. Chem.
(1989) - et al.
Intracellular cholesterol transport and compartmentation
J. Biol. Chem.
(1995) The use and abuse of filipin to localize cholesterol in membranes
Cell Biol. Int. Rep.
(1984)- et al.
Intracellular trafficking of cholesterol monitored with a cyclodextrin
J. Biol. Chem.
(1996)
Mechanism and consequences of cellular cholesterol exchange and transfer
Biochim. Biophys. Acta
Differential rate of cholesterol efflux from the apical and basolateral membranes of MDCK cells
J. Lipid Res.
Lipid domains in model and biological membranes
Chem. Phys. Lipids
Cellular cholesterol efflux mediated by cyclodextrins. Demonstration of kinetic pools and mechanism of efflux
J. Biol. Chem.
Cholesterol and the cell membrane
Biochim. Biophys. Acta
Tight junctions and the molecular basis for regulation of paracellular permeability
Am. J. Physiol.
Assembly of the tight junction: The role of diacylglycerol
J. Cell Biol.
Functional dissociation of paracellular permeability from electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant membrane protein of tight junctions
J. Cell Biol.
The effect of membrane cholesterol content on ion transport processes in plasma membranes
Cardiovasc. Res.
Protein kinase inhibitors prevent junction dissociation induced by low extra-cellular calcium in MDCK epithelial cells
J. Cell Biol.
Cited by (100)
Tight Junction Structure and Function Revisited
2020, Trends in Cell BiologyChloride channel ClC- 2 enhances intestinal epithelial tight junction barrier function via regulation of caveolin-1 and caveolar trafficking of occludin
2017, Experimental Cell ResearchCitation Excerpt :These results are consistent with the previous reports that MβCD treatment reduces TJ barrier [34,35]. MβCD treatment has been shown to cause change in occludin phosphorylation status [48], actin rearrangement [35], or proteolysis [34]. Also, following prolonged cholesterol depletion, caveolin-1 is known to be ubiquitinated and degraded [49].
Effect of polyunsaturated fatty acids (PUFAs) on airway epithelial cells' tight junction
2016, Pulmonary Pharmacology and TherapeuticsCitation Excerpt :Tight junction transmembrane proteins are located in detergent-insoluble glycolipid (DIG) rafts in the cell membrane [6]. It has also been shown that TJ proteins are in cholesterol-enriched micro-domains and a rapid reduction of membrane cholesterol by methyl-b-cyclodextrin decreases transepithelial electrical resistance (TEER) and enhances mannitol paracellular transport, modifying tight junctions integrity [7,8]. Since TJ proteins are embedded within the phospholipid bilayer of the membrane, a change in the structure of cell membrane can affect the structure and function of these transmembrane proteins.
Evidence Supporting a Lymphatic Endothelium Origin for Angiomyolipoma, a TSC2<sup>−</sup> Tumor Related to Lymphangioleiomyomatosis
2016, American Journal of PathologyConceptual barriers to understanding physical barriers
2015, Seminars in Cell and Developmental Biology
- 1
Dr. Eveline E. Schneeberger, Department of Pathology, Cox Building 5, Room 554, Massachusetts General Hospital, 100 Blossom Street, Boston, MA 02114/USA