Promotion of osteogenesis through β-catenin signaling by desferrioxamine
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Materials and methods
Cell culture and treatment. Human bone mesenchymal stem cells (hBMSCs) were isolated and expanded using the previously modified methods reported [10]. Bone marrow aspirates were obtained during routine orthopedic surgical procedures. Aspirates each amounting to 20 ml of bone marrow were harvested from three individual healthy male donors (A, 40 years old; B, 35 years old; and C, 28 years old) using a bone marrow biopsy needle inserted through the cortical bone. The aspirates were immediately
Nontoxic DFO increases BMP2-induced osteoblastic differentiation
We initially tried to determine the nontoxic concentrations of DFO on the adv-BMP2 transfected hBMSCs cells. The cells were transduced with adv-BMP2 or adv-β gal, and then were treated with various concentrations of DFO ranging from 5 μM to 100 μM for 2 days. Cell proliferation was determined by MTT assay. DFO, at a concentration ranging from 0 μM to 100 μM, inhibited cell growth in a dose-dependent manner (Fig. 1A). Cell proliferation rate was significantly lower when treated with DFO than when
Acknowledgments
This work was supported by the National Basic Research Program (973 Program) (Grant No. 2005CB522700), National Natural Science Foundation of China (Grant No. 30700402), and the Science and Technology Commission of Shanghai Municipality (Grant Nos. 05JC14034 and 07QA14062).
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MIF does only marginally enhance the pro-regenerative capacities of DFO in a mouse-osteotomy-model of compromised bone healing conditions
2022, BoneCitation Excerpt :Therefore, we selected the potent GC dexamethasone to effectively inhibit in vitro calcification of hMSCs as an in vitro model for disturbed bone synthesis to test the ability of HIF–stabilizers to re-establish osteogenic differentiation within the present study (Fig. 1). Of note, beside its stabilizing effect on HIF-1α, DFO directly influences hMSC differentiation via beta-catenin signaling cascades [41]. The counteracting potential of DFO for GC-induced inhibition of osteogenic differentiation as shown in the present study, has not been reported, yet.
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